Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-1000.0.JPG-250.jpg)
Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 1 Kilogram
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9
![Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75030-500.0.JPG-250.jpg)
Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 500 Grams
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.
HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
Teknova 10mM Tris-HCl, pH 8.0. 1000mL, DNase/RNase Tested, Sterile 6 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Alkali Scientific Murashige & Skoog Medium with MES Buffer and Vitamins, 4.9 Grams of Powder, Makes 1 Liter of Solution
Murashige & Skoog Medium with MES Buffer and Vitamins (1 liter, 4.9 grams of powder) is specially formulated for plant tissue culture. The inclusion of MES buffer helps maintain a stable pH, which is essential for controlling the growth environment of plant cells. This medium is ideal for plant growth, regeneration, and shoot multiplication, providing the necessary vitamins and nutrients for cellular metabolism and tissue development. It is commonly used in plant breeding and research for producing healthy and viable plant cultures. The formulation ensures that plant cells receive the required nutrients to thrive, making it an important tool for plant biotechnology and commercial propagation.
Alkali Scientific MOPSO Sodium Salt, 1 Kg
MOPSO Sodium Salt, 1 Kg, is an essential buffering agent used to maintain pH stability in a variety of laboratory applications, especially in protein and nucleic acid studies. The MOPSO buffer provides reliable pH control in the range of 6.5 to 7.9, making it an ideal choice for enzyme assays, protein extractions, and other biochemical processes. The 1 kg size is perfect for large-scale experiments or labs that require frequent use of this buffer for ongoing research. MOPSO is also widely used in pharmaceutical and biotechnology labs, where consistent pH levels are crucial for reproducible results in complex biological assays.
Medchemexpress LLC BES sodium | 66992-27-6 | 97.0% | 25 G
BES sodium is a secondary standard biochemical buffer with an effective pH range of 6.4 to 7.8 (pKa: 7.15 at 20 °C). It is used in the diagnostic testing area.
- Biochemical buffer
- Effective pH range: 6.4 to 7.8 (pKa: 7.15 at 20 °C)
- Used in diagnostic testing
- Appearance: Solid
- Color: White to off-white
- Target: Biochemical assay reagents
- Pathway: Others
Alkali Scientific Murashige & Skoog Medium with MES Buffer, 24 Grams of Powder, Makes 5 Liters of Solution
Murashige & Skoog Medium with MES Buffer is designed for plant tissue culture and is available in a 5-liter size. This powder, containing 24 grams, makes 5 liters of solution, providing essential nutrients and pH stability with the inclusion of MES buffer. The buffer ensures the pH remains constant, which is vital for maintaining optimal growth conditions for plant cells. This medium is widely used in laboratories for plant cell culture, genetic studies, and regeneration research. MES buffer is particularly important in experiments that require precise pH control, making this medium an essential tool for researchers working with plant tissue cultures.
Chem-Impex International, Inc. 2-Morpholinoethanesulfonic acid | 4432-31-9 | MFCD00006181 | 100G
2-Morpholinoethanesulfonic acid, 4432-31-9, MFCD00006181, 100G
Hampton Research StockOptions CHES Buffer Kit, pH 8.6 to 10.0 in 0.1 pH unit increments
StockOptions CHES Buffer Kit, pH 8.6 to 10.0 in 0.1 pH unit increments