Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific CellPro™ Tris-HCL Buffer, 5M pH 7.4, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Seracare Life Sciences Inc 10X TRIS-GLYCINE TRANS BUFFER
10X Tris-glycine Transfer Buffer is intended for Western blotting and gel electrophoresis. It is ideal for preparing a standard Western blot transfer buffer (Towbin) and for use as a gel electrophoresis buffer for native Tris-glycine gel without SDS. pH=8.3. Conductivity controlled.
![Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60050-5000.0.JPG-250.jpg)
Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 5 Kilograms
TRIS Hydrochloride, Tris-HCl, is a buffering agent commonly utilized to maintain a stable pH in experimental procedures.
It is primarily used as a buffering agent for applications that require a slightly alkaline to neutral pH range. Also, It is effective in the pH range of 7.2 to 9.0, making it suitable for a variety of biochemical and molecular biology techniques.
TRIS HCl is commonly used in DNA and RNA extraction, protein purification, enzyme assays, and electrophoresis. It helps maintain the desired pH conditions for enzymatic reactions, stability of nucleic acids, and separation of proteins during electrophoresis.
One of the advantages of TRIS HCl is its ability to resist changes in pH caused by the addition of acids or bases, making it a reliable buffering agent. It also exhibits low ionic strength, which minimizes interference with biological systems and enzymatic activities.
Alkali Scientific QuickSilver TBS Buffer Powder, 100 Pouches
QuickSilver™ Buffer Powder packs offer extreme convenience and will change the way you prepare buffers in your lab. They are quick, and easy to use. Convenient premeasured pouches make 1L of 1X buffer. There is no pH adjusent needed and they offer a long shelf life. They come supplied with preprinterd bottle labels for easy identification. 100 pouches per pack.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.6) - 1L
Tris-HCl Buffer (1 M pH 7 6) - 1L (For Research Use and Further Manufacturing Only)
Alkali Scientific CellPro™ Tris Buffered Saline 10X, pH 7.4, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Bioworld Tris Buffered Saline Packs, 10 PK(s)
Tris Bufferd Saline Packs An ideal all-purpose binding buffer.Each pack yields 500 ml of 25 mM Tris, 0.15 M NaCl, pH 7.2 when dissolved in 500 ml deionized water (total 5 liters). Quantity: 10 packs
Cell Signaling Technology Tris-Glycine SDS Running Buffer (10X) 1000 ml
Tris-Glycine SDS Running Buffer (10X) 1000 ml
Medchemexpress LLC P62-mediated mitopha 10mM 1mL | 1809031-84-2 | 392.15 g·mol⁻1 | C14H9IN4O2 | 1 ML
P62-mediated mitophagy inducer is a small-molecule research reagent that activates p62-mediated mitophagy without recruiting Parkin or collapsing mitochondrial membrane potential. It is supplied as a 10 mM stock solution (1 mL) for use in cellular and biochemical studies of selective mitochondrial clearance and autophagy pathways.
- Activates p62-mediated mitophagy without Parkin recruitment.
- Provided as a ready-to-use 10 mM stock solution in 1 mL.
- Suitable for cellular and biochemical assays investigating mitophagy and autophagy.
- Stable storage recommendations: powder at -20°C for up to 3 years; in solvent at -80°C or -20°C as indicated.
- Comes with chemical identification and characterization data (CAS, formula, molecular weight) in the supplier datasheet.
![Research Products International Corp TBE Buffer [TRIS-Borate-EDTA], 10X Powder, 170.3 Grams of Powder Makes 1 Liter of Solution](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T22020-1.0.JPG-250.jpg)
Research Products International Corp TBE Buffer [TRIS-Borate-EDTA], 10X Powder, 170.3 Grams of Powder Makes 1 Liter of Solution
Dry homogeneous blend of molecular biology grade TRIS base, Boric Acid and EDTA.
For use in gel electrophoresis.
Boston Bioproducts Inc Tris Buffered Saline, TBS (10X, pH 7.6) - 1L
Tris Buffered Saline TBS (10X pH 7 6) - 1L (For Research Use and Further Manufacturing Only)
Pfanstiehl Inc Tris Base (Tromethamine) High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP (T-153) 1 Kg GMP, Multi-compendial CAS: 77-86-1 Injectable Excipient Grade
Pfanstiehl Tris Base, High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP is a multi-compendial buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris Base meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. Pfanstiehl Brand Tris Base is compliant with USP (United States Pharmacopoeia) EP (European Pharmacopoeia), BP (British Pharmacopeia) and ChP (Chinese Pharmacopeia) Compendial, U.S.P. , cGMP , pharmaceutical grade .
S2 Media 20x Tris Buffered Saline (400mM Tris-HCl, 3M NaCl), pH 7.4, Sterile, 1L Media Bottle, 1ea
Tris Buffered Saline (TBS), formulated at a 20x concentration, is used in cell culture, molecular biology, protein research, and purification applications. It can be utilized as a wash buffer, storage buffer, and for resuspension of cells. he Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical applications. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration.• Tris Buffered Saline should be used by trained professionals within a laboratory setting. • Tris is an abbreviation for tris(hydroxymethyl)aminomethane).• Product is not intended for use in the diagnosis or treatment of disease or other human conditions.• For Research Use Only (RUO). Not for use in diagnostic procedures.