Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences MES Monohydrate | 145224-94-8 | MFCD00149409 | 100g
MES Monohydrate | Purity: 99% | Mol Wt: 213.25 | 145224-94-8 | MFCD00149409 | 100g
Sigma Aldrich Fine Chemicals Biosciences MOPS sodium salt BioPerformance Certified, suitable for cell culture, >=99.5% | 71119-22-7 | MFCD00064350 | 1KG
MOPS sodium salt BioPerformance Certified, suitable for cell culture, >=99.5% | Purity: >=99.5% | Mol Wt: 231.25 | 71119-22-7 | MFCD00064350 | 1KG
Sigma Aldrich Fine Chemicals Biosciences CAPS | 1135-40-6 | MFCD00003837 | 250g
CAPS | Purity: 99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 250g
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS Propane | 64431-96-5 | MFCD00004689 | 100g
BIS-TRIS Propane | Purity: 99% | Mol Wt: 282.33 | 64431-96-5 | MFCD00004689 | 100g
Sigma Aldrich Fine Chemicals Biosciences CAPS anhydrous, free-flowing, Redi-Dri(TM), >=99% | 1135-40-6 | MFCD00003837 | 500G
CAPS anhydrous, free-flowing, Redi-Dri(TM), >=99% | Purity: >=99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 500G
Sigma Aldrich Fine Chemicals Biosciences HEPES buffer solution 1 M in H2O | 7365-45-9 | MFCD00006158 | 500ML
HEPES buffer solution 1 M in H2O | 7365-45-9 | MFCD00006158 | 500ML
Sigma Aldrich Fine Chemicals Biosciences BICINE | 150-25-4 | MFCD00004295 | 100g
BICINE | Purity: 99% | Mol Wt: 163.17 | 150-25-4 | MFCD00004295 | 100g
Sigma Aldrich Fine Chemicals Biosciences CHES BioXtra, >=99.0% (titration) | 103-47-9 | MFCD00003835 | 25G
CHES BioXtra, >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 25G
Sigma Aldrich Fine Chemicals Biosciences MES monohydrate | 145224-94-8 | MFCD00149409 | 500g
MES monohydrate | Purity: 99% | Mol Wt: 213.25 | 145224-94-8 | MFCD00149409 | 500g
Sigma Aldrich Fine Chemicals Biosciences Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | 1185-53-1 | MFCD00012590 | 1KG
Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 157.6 | 1185-53-1 | MFCD00012590 | 1KG
Sigma Aldrich Fine Chemicals Biosciences HEPES BioUltra, for molecular biology, 99.5 (T), 7365-45-9, MFCD00006158
HEPES BioUltra, for molecular biology, 99.5 (T)
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS 98.0 (titration), 6976-37-0, MFCD00002853
BIS-TRIS 98.0 (titration)
Boca Scientific Inc Antibody Stabilizer PBS Base, 125ml
Antibody Stabilizer conserves the structure of proteins and antibodies to prevent loss of functionality due to storage. With refrigerated storage, Antibody Stabilizer prevents the need for freezing and thawing. Both TRIS-based and PBS-based Antibody Stabilizers are available, allowing the user to select the optimal buffer base for the assay.
Quality Biological Inc Tris HCL (1 M), pH 8.0, 500 mL
A hybridization buffer specifically formulated for use with nylon membranes. ¿ It does not contain Denhardt¿s Reagent, which may interfere with the hybridization reaction of DNA immobilized on nylon. ¿ Formulated for Nylon membrane ¿ Filtered through a 0.22 ¿m membrane ¿ DNase, RNase, and Protease tested ¿ Applications: Denatured fragmented salmon sperm DNA may be added to this hybridization buffer to reduce nonspecific background. ¿ Storage Conditions: 25¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
