Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Axxora LLC Hepes Buffer 1 M pH 7.5
Hepes Buffer is one of the best general purpose buffers available for biological research. It has been used to advantage in tissue culture, oxidative phosphorylation and protein synthesis with cell-free bacterial systems. Hepes is a recommended buffer for the glutamate binding assay, prevents binding to non-receptor materials.
Growcells PBS 20X 10L EA
Sterile 20X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 160g, KCl 4g, Na2HPO4 28.8g, KH2PO4 4.8g
Alkali Scientific CellPro™ 1X DPBS, Dulbecco’s Phosphate Buffered Saline, without Calcium or Magnesium, Sterile Filtered, 1L, 10/Case
Like PBS, DPBS is also a water-based solution containing sodium chloride and phosphate buffer that is used in biological applications. However, DPBS differs from PBS in that it also includes potassium chloride and is available in a larger variety of formulations.
Sigma Aldrich Fine Chemicals Biosciences Tris-Borate-EDTA buffer | MFCD00236358 | 4 L
Tris-Borate-EDTA buffer | MFCD00236358 | 4 L
Nusep Inc NUVIEW MINI GEL 4-20 10-WELL
NuSep's nUView Tris-Glycine SDS-PAGE Precast Gel 4-20% 10well. Cassette size: 10 x 8.5 cm. Fits Mini-PRO or Bio-Rad's Mini-Protean Tetra cell Tanks. 10 gel cassettes per box. All nUView™ precast gels incorporate a unique formulation allowing protein bands to be visualized in only 2 minutes in the presence of ultraviolet (UV) light. Simply place your gel over a 250-320 nm UV trans-illuminator and watch high-resolution bands appear in just 2 minutes. With nUView™ technology you no longer have to stain and de-stain your gels – increasing protein recovery and saving you significant time while receiving improved results.
Sigma Aldrich Fine Chemicals Biosciences Trizma base, 77-86-1, MFCD00004679, 100 g
Linear Formula: NH2C(CH2OH)3, Molecular Weight: 121.14, BioPerformance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9% (titration), Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol.
Teknova 1M Tris-HCl, pH 9.0. 500 mL, Sterile. 4 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova Assay Diluent. 1000mL, Sterile. 2 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Teknova HBS-EP Buffer (10mM Hepes, 150mM NaCl, 3mM EDTA, 0.005% Tween-20) 200mL, Sterile. 10 pack.
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
Teknova 1M Tris-HCl, pH 8.2. 1000mL, Sterile. 2 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.