Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine HEPES or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Alkali Scientific CellPro™ PBS 1X, pH 7.4, with Sodium Azide, FAcs Washing Grade, 500mL
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
![Alkali Scientific PBS [Phosphate Buffered Saline] 200mL Tablets, 100 Tablets per Bottle](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661398.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline] 200mL Tablets, 100 Tablets per Bottle
PBS [Phosphate Buffered Saline] 200mL Tablets are solid tablets designed for easy dissolution in water, making it simple to prepare 200mL of phosphate-buffered saline. These tablets are ideal for laboratories that frequently need to prepare large volumes of PBS for use in cell culture, microbiology, and molecular biology research. The tablets dissolve quickly and efficiently, ensuring that the resulting solution maintains the proper pH and osmolarity for reliable experimental results. Each bottle contains 100 tablets, providing an economical and convenient way to prepare PBS without the need for precise measuring or mixing of individual components.
Alkali Scientific CellPro™ 1X DPBS, Dulbecco’s Phosphate Buffered Saline, without Calcium or Magnesium, Sterile Filtered
1X DPBS, Dulbeccos Phosphate Buffered Saline, without Calcium or Magnesium, sterile Filtered, 500mL, 1ea
Mitegen HEPES/ NaOH, 1M, pH7.0, 250 ml, CAS # 7365-45-9
Use our stock solutions directly or as a component in solutions you create in your own lab. We prepare our stock solutions using high quality raw materials, ASTM Type 1 water, and sterile packaging.�Our stock solutions are intended for Research and Development Use Only.
Sigma Aldrich Fine Chemicals Biosciences Trizma base, 77-86-1, MFCD00004679, 100 g
Linear Formula: NH2C(CH2OH)3, Molecular Weight: 121.14, BioPerformance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9% (titration), Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol.
Rockland Immunochemicals 1.0 M Tris HCl pH 7.4 - MB-002
Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of additional buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids. Tris HCl solution (tris(hydroxymethyl)aminomethane hydrochloride) is useful for stabilization of buffer formulations and electrophoresis of biological molecules. Visit our newly expanded web site at www.rockland-inc.com for methods using this and other buffers.
Supply Solutions Sigma-Aldrich -500ml Tris-EDTA buffer solution BioUltra, for molecular biology, pH 8.0 (Fluka) * Third Party Item
Sigma-Aldrich -500ml Tris-EDTA buffer solution BioUltra, for molecular biology, pH 8.0 (Fluka) * Third Party Item
Sigma Aldrich Fine Chemicals Biosciences HEPES anhydrous, free-flowing, Redi-Dri(TM), >=99.5% | 7365-45-9 | MFCD00006158 | 1KG
HEPES anhydrous, free-flowing, Redi-Dri(TM), >=99.5% | Purity: >=99.5% | Mol Wt: 238.3 | 7365-45-9 | MFCD00006158 | 1KG
Sigma Aldrich Fine Chemicals Biosciences Tris-EDTA Buffer | CAS No.:Not specified| MDL No.: MFCD00236359 | 1 L
Tris-EDTA buffer | Purity: 100% | M. W.: Not applicable | CAS No.: Not Specified| MDL No.: MFCD00236359 | 1L
![Research Products International Corp bis-TRIS [bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B75000-100.0.JPG-250.jpg)
Research Products International Corp bis-TRIS [bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane], 100 Grams
Substance Name: bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane
CAS Number: 6976-37-0
Molecular Formula: C8H19NO5
Molecular Weight:?209
Chemical Purity:?>99%
Soluble in: Water
Store at:?Room Temperature