Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc PIPES Buffer (0.5 M, pH 7.0) - 100ml
The PIPES Buffer is a zwitterionic buffering agent commonly used in biochemistry and molecular biology for its ability to maintain a stable pH. With a pKa of approximately 6.8, PIPES is effective in the pH range of 6.1 to 7.5, making it suitable for cell culture, enzyme assays, protein purification, and as a running buffer in gel electrophoresis. PIPES is also used in electron microscopy, protein crystallization, and isoelectric focusing and chromatography.
Thermo Scientific Production Chemicals and Services 50 MM TRIS-HCL 10 MM EDTAPH8
NC3588253 50 MM TRIS-HCL 10 MM EDTAPH8
Thermo Scientific Production Chemicals and Services 50MM TRIS 10 MM EDTA 1.65M AM
NC3588257 50MM TRIS 10 MM EDTA 1.65M AM
Thermo Scientific Production Chemicals and Services 50 MM TRIS-HCL 10 MM EDTA PH
NC3588258 50 MM TRIS-HCL 10 MM EDTA PH
Thermo Scientific Production Chemicals and Services 50MM TRIS 10MM EDTA PH 7.2 1
NC3588260 50MM TRIS 10MM EDTA PH 7.2 1
Thermo Scientific Production Chemicals and Services 50MM TRIS-HCL 10MM EDTA 0.6M
NC3588264 50MM TRIS-HCL 10MM EDTA 0.6M
Thermo Scientific Production Chemicals and Services 50 MM TRIS-HCL 10 M EDTAPH7.2
NC3588267 50 MM TRIS-HCL 10 M EDTAPH7.2
Thermo Scientific Production Chemicals and Services 50MM TRIS-HCL 10MM EDTA 0.6M
NC3588268 50MM TRIS-HCL 10MM EDTA 0.6M
Thermo Scientific Production Chemicals and Services 50 MM TRIS-HCL 10 MM EDTAPH8
NC3588273 50 MM TRIS-HCL 10 MM EDTAPH8
Boston Bioproducts Inc HEPES Buffer, 1 M, pH 7.5, 500 mL
This product is a 1 M HEPES Buffer solution, pH 7.5, which is essential for maintaining stable physiological pH in various biological applications. It is rigorously quality-controlled and specifically formulated for precision in sensitive biochemical and molecular biology research. The high-purity zwitterionic buffering agent is ready-to-use and suitable for diverse applications such as cell culture, protein purification, and live cell imaging.
- Stable pH control maintaining 6.8 to 8.2
- Broad applicability in various research areas
- Convenient format as a ready-to-use solution
- High purity, formulated with USP Compendial-Grade raw materials
- CO₂-independent buffering for reliable pH stability
- Filtered through a 0.22-micron filter for enhanced purity