Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Enzo Life Sciences Ada-Tyr-(Ahx)3-(Leu)3-vinyl sulfone (100µg)
A highly potent, cell-permeable proteasome inhibitor that targets all individual active subunits with comparable affinity. This extended peptide-based inhibitor can be radioiodinated, allowing detection of labeled subunits by autoradiography. Purity: ≥97%. Long Term Storage: -20°C.
![Research Products International Corp CHES [2-(N-Cyclohexylamino)ethanesulfonic acid], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C40020-1000.0.JPG-250.jpg)
Research Products International Corp CHES [2-(N-Cyclohexylamino)ethanesulfonic acid], 1 Kilogram
CHES, also known as 2-(N-Cyclohexylamino) ethanesulfonic acid, is a chemical compound that plays a significant role in life science research, particularly in molecular biology and protein research.
CHES is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 8.6 to 10.0, making it suitable for applications requiring higher alkaline pH conditions.
CHES is also commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability in alkaline conditions make it valuable in these applications.
One of the significant advantages of CHES is its low ionic strength, which minimizes interference with biological processes and enzymatic activities.
Sigma Aldrich Fine Chemicals Biosciences HEPBS >=99% (titration) | 161308-36-7 | MFCD00677124 | 25G
HEPBS >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 266.36 | 161308-36-7 | MFCD00677124 | 25G
Medchemexpress LLC MES sodium salt | 71119-23-8 | 98.0% | 50 G
MES sodium salt | 71119-23-8 | 98.0% | 50 G
Bioworld ADA (N-(2-acetamido)iminodiacetic acid) Buffer, 0.2M, pH 7.0, 500 mL
Synonym:ADA-Na,N-(2-Acetamido)iminodiacetic acid monosodium salt,N-(Carbamoylmethyl)iminodiacetic acid monosodium saltA zwitterionic buffer used in biological applications including protein isolation and characterizationWarning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption. Appearance: LiquidPH Value: 7.0
![Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid] sodium salt, 5 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659869.jpg-250.jpg)
Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid] sodium salt, 5 Kg
MES Sodium Salt [2-(N-Morpholino)ethanesulfonic acid] Sodium Salt, 5 Kg, is a widely used buffering reagent known for its ability to stabilize pH in a variety of biological and biochemical applications. With a high buffering capacity, MES is effective in maintaining pH in the range of 5.5 to 6.7, making it ideal for enzyme reactions, electrophoresis, and other experiments requiring precise pH control. The 5 kg weight offers a cost-effective solution for large-scale operations or frequent use. MES is often used in protein chemistry, molecular biology, and cell culture media to ensure consistent conditions during experimental procedures.
Chem-Impex International, Inc. MOPS sodium salt | 71119-22-7 | MFCD00064350 | 25G
MOPS sodium salt, 71119-22-7, MFCD00064350, 25G
Sigma Aldrich Fine Chemicals Biosciences BES 99.0 titration250G
BES (N N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) is a useful secondary standard biochemical buffer. Useful pH range for BES is 6.4 to 7.8. It is useful for diagnostic assay manufacturing industry. BES a sulfonic acid-containing cross-linking agent induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes.
Mitegen MES/ NaOH, 1M, pH6.0, 250 ml, CAS # 4432-31-9
Use our stock solutions directly or as a component in solutions you create in your own lab. We prepare our stock solutions using high quality raw materials, ASTM Type 1 water, and sterile packaging.�Our stock solutions are intended for Research and Development Use Only.
Teknova 2M MOPS Buffer, pH 7.4. 500mL, Sterile 8 pack.
MOPS (3-(N-morpholino)propanesulfonic acid) is a Good’s buffer and has a pKa of 7.01, making it an ideal buffer for many biological systems at near-neutral pH. MOPS can be used in cell culture media and biopharma buffer formulations as well as in the purification of antibodies, peptides, proteins and blood components. It is nontoxic to cell culture lines.
Medchemexpress LLC 4-Morpholineethanesulfonic acid, sodium salt (2:1) | 117961-21-4 | 98.0% | 25 G
MES (2-Morpholinoethanesulphonic acid) hemisodium is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5 to 7.0. It is a Good's buffer widely used in biochemistry and molecular biology experiments.
- Amphoteric ion buffer.
- Buffer capacity ranging pH 5.5-7.0.
- Widely used in biochemistry and molecular biology experiments, such as cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Zwitterionic buffer, providing effective buffering capacity around pH 6.15.
- pKa value changes with temperature, requiring consideration for different temperatures.
- Shows good buffering properties in biological experiments.
- Exhibits poor affinity with metal ions (binding constants at 20 °C and 0.1 M: Mg2+, 0.8; Ca2+, 0.8; Mn2+, 1.7), making it suitable for studies involving metal ions.
![Research Products International Corp RPI PIPES [Piperazine-N-N'-bis(2-ethanesulfonic acid)], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P40140-5000.0.JPG-250.jpg)
Research Products International Corp RPI PIPES [Piperazine-N-N'-bis(2-ethanesulfonic acid)], 5 Kilograms
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
Medchemexpress LLC Tapso | 68399-81-5 | 98.0% | 100 G
TAPSO is a widely used biological buffer and pH stabilization reagent. It offers an effective pH range between 7 and 8, with pKa values typically falling between 7.5 and 9.0. This compound contains Tris groups and has been observed to exhibit reactive activity with zwitterionic glycine peptides. It is primarily intended for research applications.
- Effective pH range of 7-8.
- pKa values ranging from 7.5-9.0.
- Contains Tris groups.
- Shows reactive activity with zwitterionic glycine peptides.
- Suitable for various research applications.
Research Products International Corp PIPES FREE ACID ULTRAPURE, 250 GM
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5