Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc TRIS-Acetate-SDS Running Buffer (10X), 500 ml
TRIS-Acetate-SDS Running Buffer (10X), 500 ml.
Biotang Inc E. Coli Lysis Buffer (Tris-HCl 50 mM,1% Triton,EDTA 1 mM, Beta-mercaptoethanol 10 mM), 250 ml
E. Coli Lysis Buffer (Tris-HCl 50 mM,1% Triton,EDTA 1 mM, Beta-mercaptoethanol 10 mM), 250 ml.
Sigma Aldrich Fine Chemicals Biosciences PIPES BioPerformance Certi1KG
PIPES BioPerformance Certi1KG
Medchemexpress LLC Tris buffered saline | 10 TABLETS
Tris buffered saline is supplied as ready-to-dissolve tablets for preparing Tris/NaCl buffer solutions commonly used in molecular biology and protein applications. The tablet format simplifies buffer preparation and storage while delivering a stable pH environment for assays and electrophoresis.
- Ready-to-use tablet format for convenient buffer preparation.
- One tablet dissolves in 15 mL deionized water to yield 0.05 M Tris and 0.15 M sodium chloride.
- pH approximately 7.6 at 25 °C.
- White to off-white solid tablets for easy visual quality check.
- Suitable for DNA/RNA extraction, PCR, and protein electrophoresis.
- Compact packaging reduces storage space and eases transport.
Medchemexpress LLC Mes (2-morpholinoethanesulphonic acid) | 4432-31-9 | 99.85% | 1 ML
MES (2-Morpholinoethanesulphonic acid) is an amphoteric ion buffer designed for various biochemistry and molecular biology applications. It functions effectively within a pH range of 5.5-7.0 and is widely recognized as a Good's buffer, suitable for experiments including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer
- Buffer capacity from pH 5.5-7.0
- Ideal for cell culture, enzyme activity determination, electrophoresis, and protein studies
- Solid, white to off-white appearance
- Soluble in water and DMSO
Medchemexpress LLC Heppso | 68399-78-0 | 99.89% | 100 G
HEPPSO is a zwitterionic buffer with a working pH range of 7.1-8.5. It exhibits a relatively high ability to bind copper(II) and possesses a pKa of 7.84 at a 2.0 mM buffer concentration. It is intended for research use only and is not sold to patients.
- Working pH range of 7.1-8.5.
- Exhibits a relatively high ability to bind copper(II).
- Possesses a pKa of 7.84 at a 2.0 mM buffer concentration.
- For research use only.
- Molecular weight: 268.33.
- Formula: C9H20N2O5S.
- Appearance: Solid.
- Color: White to off-white.
Sigma Aldrich Fine Chemicals Biosciences PIPES BioPerformance Certified, suitable for cell culture | 5625-37-6 | MFCD00006159 | 25G
PIPES BioPerformance Certified, suitable for cell culture | Mol Wt: 302.37 | 5625-37-6 | MFCD00006159 | 25G
Medchemexpress LLC 2-Morpholinoethanesulphonic acid hemisodium | 117961-21-4 | 98.0% | 100 G
MES (2-Morpholinoethanesulphonic acid) hemisodium is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. As a Good's buffer, it is widely utilized in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- It is a zwitterionic buffer and provides effective buffering capacity around pH 6.15.
- The pKa value changes with temperature, requiring consideration for its use at different temperatures.
- It shows good buffering properties in biological experiments.
- It exhibits poor affinity with metal ions, allowing its use in studies involving metal ions.
Gold Biotechnology Inc MOPS Free Acid Ultra Pure
MOPS is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic morpholinic buffer that is useful for a pH range of 6 5 7 9 and commonly used for cell culture media as a running buffer in electrophoresis and for protein purification in chromatography MOPS lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions
Gold Biotechnology Inc HEPES Free Acid 100 g
HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic piperazinic buffer that is useful for a pH range of 6 8 8 2 HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions
Boca Scientific Inc Antibody Stabilizer PBS Base, 125ml
Antibody Stabilizer conserves the structure of proteins and antibodies to prevent loss of functionality due to storage. With refrigerated storage, Antibody Stabilizer prevents the need for freezing and thawing. Both TRIS-based and PBS-based Antibody Stabilizers are available, allowing the user to select the optimal buffer base for the assay.