Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC PbS/CdS QDs (PbS/CdSe QD) | PbS/CdS | 10 MG
PbS/CdS quantum dots are colloidal nanocrystals designed as a fluorescent probe for in vivo imaging in the second near-infrared (NIR-II) window. These aqueous suspensions emit near ~1300 nm, yielding high signal-to-background ratio and reduced tissue scattering for deeper, clearer fluorescence imaging. The product is supplied as a light yellow to brown liquid suspension at 10 mg/mL and requires protection from light during storage.
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered) - 20L-CBY
Phosphate Buffered Saline PBS (pH 7 4 Sterile-Filtered) - 20L-CBY (For Research Use and Further Manufacturing Only)
Teknova 150mM PBS, pH 6.0. 5L, Sterile 2 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Apexbio Technology LLC Annexin V-HF647/7-AAD Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF647) can be used as a probe in combination with flow cytometry or fluorescence microscopy for detecting apoptosis and the staining process requires only 10-20 minutes 7-AAD specifically binds double-stranded DNA emitting red fluorescence Normally 7-AAD cannot penetrate intact cell membranes However in late-apoptotic and dead cells membrane integrity is compromised allowing 7-AAD to enter and stain nuclear DNA Co-staining with Annexin V-HF647 and 7-AAD thus differentiates cells in various apoptotic stages and dead cells
FUJIFILM BIOSCIENCES INC MEM 1X Earles Salts w/o NEAA
MEM 1X Earles Salts w/o NEAA is an uncomplicated medium that is useful for a wide range of species and cells
Ethos Biosciences Dilurex 10X PBS Buffer, 100ml, Ethos
Dilurex 10X PBS is a concentrated solution of phosphate buffered saline intended as a stock solution. Save space by keeping 10x concentrate and dilute to 1x pH 7.4 working concentration when needed by mixing 1 part 10X PBS with 9 parts distilled water. Skip the tedium of weighing powders and stocking raw materials that can expire. Our PBS is convenient, ready to use, and will save you hours in the lab, letting you focus on breakthroughs rather than buffer prep. Thousands of researchers trust Ethos Biosciences 10X PBS for its proven track record in top-tier publications and protocols - upgrade your productivity today.
Medchemexpress LLC Phosphate-Buffered Saline | 500 ML
Phosphate-Buffered Saline (PBS) is a balanced salt solution designed for various cell culture applications. These applications include washing cells, diluting cells, and preparing reagents. The standard base specification for this product is 500 mL, with larger sizes being incremental volumes of this base.
- Filtered and ready to use for general cell culture purposes.
- Free of Mg2+, Ca2+, and phenol red.
- pH is 7.2-7.4.
ILEX LIFE SCIENCES LLC TRYPSIN P10-021100 6 X 100ML
PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme in PBS solution. It is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. APPLICATION: Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.25% Trypsin. EDTA: No EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Alkali Scientific CellPro™ DPBS Buffer, 1X, with Calcium and Magnesium, 1L, 10/Case
Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.
Cayman Chemical oloyl EthnolamID phsphate 10mg
An LPA mimetic, LPA receptor agonist, and phosphate ester of OEA; selectively increases [35S]GTPγS binding to HEK293T cell membranes expressing LPA1 and LPA2 over LPA3 receptors; induces calcium mobilization and inhibits forskolin-induced cAMP accumulation in MDA-MB-231 cells (EC50s = 1.2 and 101 nM, respectively)
Boston Bioproducts Inc 10X Dulbecco's Phosphate Buffered Saline, DPBS (no Calcium, no Magnesium) - 2x1L
Bioprocess Grade - 10X Dulbecco s Phosphate Buffered Saline DPBS (no Calcium no Magnesium) - 2x1L (For Research Use and Further Manufacturing Only)
Medchemexpress LLC MOPS sodium salt | 71119-22-7 | 99.8% | 500 G
MOPS sodium salt is a buffering agent used in biology. MOPS buffer maintains the pH of mammalian cell culture media. It significantly improves the thermal stability of BSA. MOPS sodium salt is relatively safe for short-term experiments, but it may significantly reduce cell viability after 4 hours, so it is recommended to limit the experiment duration to 4-12 hours.
- Purity: 99.8%
- Appearance: Solid
- Color: White to off-white
- Storage: 4°C, sealed, away from moisture. In solvent: -80°C for 6 months; -20°C for 1 month (sealed, away from moisture).
- Solubility in H2O: 100 mg/mL (needs ultrasonic)
- Solubility in DMSO: 50 mg/mL (needs ultrasonic; use newly opened DMSO)
Alkali Scientific ADA, [N (Carbamoylmethyl) iminodiacetic acid), 100 G
ADA (N-(Carbamoylmethyl) iminodiacetic acid) is a biochemical reagent available in a 100g bottle. This buffering agent is commonly used in laboratory settings for maintaining a stable pH in aqueous solutions. ADA is often employed in chromatography, enzyme assays, and protein purification where precise pH control is essential. It works effectively in various biochemical and molecular biology applications, providing a stable environment for enzymes and proteins. The 100g size is ideal for research laboratories and industrial applications requiring high-quality reagents for pH regulation and buffering in biochemical processes.
Medchemexpress LLC 2-Morpholinoethanesulphonic acid potassium | 39946-25-3 | 99.9% | 50 G
MES potassium is an amphoteric ion buffer widely used in biochemistry and molecular biology experiments. It provides effective buffering capacity around pH 6.15 and is suitable for cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer
- Features a buffer capacity ranging from pH 5.5-7.0
- Functions as a zwitterionic buffer
- Exhibits good buffering properties in biological experiments
- Shows poor affinity with metal ions, suitable for metal ion studies