Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.0) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Research Products International Corp TBE, 4 L Cube
TBE is a common buffer used in gel electrophoresis.
Supplied as a 1X concentrated solution containing:
89mM Boric Acid
2mM EDTA disodium salt
pH (1X solution @ 25°:C): 8.3+0.1
Research Products International Corp TG 10X Solution, 2 L
10X Formulation:
Glycine: 1.92 M
pH: 8.5
Add methanol according to protocol
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.8) - 1L
Tris-HCl Buffer (1 M pH 7 8) - 1L (For Research Use and Further Manufacturing Only)
Sigma Aldrich Fine Chemicals Biosciences MES hydrate BioUltra | 1266615-59-1 | MFCD00149409 | 250g
MES hydrate BioUltra | 1266615-59-1 | MFCD00149409 | 250g
Seracare Life Sciences Inc 10X TRIS BUFFERED SALINE (TBS)
10X Tris-buffered Saline (TBS) is used as a general wash buffer in immunoassay applications.
Seracare Life Sciences Inc 10X DULBECCO S PBS (D-PBS)
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications as well as tissue and cell culture procedures. This modified Dulbecco's buffer is not formulated with magnesium or calcium salts. pH=7.4. Conductivity controlled.
Sigma Aldrich Fine Chemicals Biosciences 5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride >=85% (HPLC) | 90217-02-0 | MFCD00467521 | 50MG
5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride >=85% (HPLC) | Purity: >=85% (HPLC) | Mol Wt: 311.77 | 90217-02-0 | MFCD00467521 | 50MG
HIMEDIA Dulbeccos Phosphate Buffered
Dulbecco s Phosphate Buffered Saline 1X w/o Phenol red Calcium and Magnesium
Research Products International Corp HEPES, Sodium Salt, 5 KG
pKa (20°C): 7.45-7.65
pH Range: 6.8-8.2
![Research Products International Corp RPI MOPS [3-(N-morpholino)-propanesulfonic acid], 10 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-10000.0.JPG-250.jpg)
Research Products International Corp RPI MOPS [3-(N-morpholino)-propanesulfonic acid], 10 Kilograms
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9