Life Science Buffers
ALADDIN SCIENTIFIC CORPORATION MES, 100g
useful pH range 5 5 - 6 7 pKa 6 1 MES is one of the Good s buffers the most extensively used biological buffers MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring It does not form complexes with majority of the metals used in environmental and biological studies It is easily soluble in water and has minimum lipid solubility making it impermeable to membranes product description MES is one of the Good s buffers the most extensively used biological buffers MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring It does not form complexes with majority of the metals used in environmental and biological studies It is easily soluble in water and has minimum lipid solubility making it impermeable to membranes
Alkali Scientific CellPro™ PBS, 10X, pH 7.4, 500mL, 500ml, 10/case
CellPro™ PBS, 10X, pH7.4, 500mL Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.
ILEX LIFE SCIENCES LLC GELATIN SOLUTION 0.1 6X500ML
PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg (6 x 500 ml). DESCRIPTION: PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg for laboratory research applications. Gelatin is a heterogenous mixture of water soluble proteins of high average molecular weight present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, or bones in water. APPLICATION: Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to stabilize Taq DNA Polymerase, and use as a blocking agent in Western Blotting, ELISA, and immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media. STORAGE: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
Chem-Impex International, Inc. 2-(N-Cyclohexylamino)ethanesulfonic acid | 103-47-9 | MFCD00003835 | 100G
2-(N-Cyclohexylamino)ethanesulfonic acid, 103-47-9, MFCD00003835, 100G
ILEX LIFE SCIENCES LLC GELATIN SOLUTION 2.0 6 X 100ML
PAN-Biotech Gelatin Solution 2% in DPBS, w/o: Ca, Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech Gelatin Solution 2% in DPBS, w/o: Ca, Mg for laboratory research applications. Gelatin is a heterogenous mixture of water soluble proteins of high average molecular weight present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, or bones in water. APPLICATION: Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to stabilize Taq DNA Polymerase, and use as a blocking agent in Western Blotting, ELISA, and immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media. STORAGE: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
Apexbio Technology LLC Annexin V-Cy5/PI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Under normal conditions PS resides on the inner side of the cell membrane lipid bilayer However in early apoptosis PS translocates from the inner leaflet of the plasma membrane to the outer surface becoming exposed externally Annexin V exhibits high binding affinity for PS and when conjugated with fluorescent molecules (Cy5) it serves as a fluorescent probe to detect apoptosis using flow cytometry or fluorescence microscopy The staining procedure requires only 10 20 minutes PI specifically binds to double-stranded DNA to produce red fluorescence it is normally impermeant to intact cell membranes However during late-stage apoptosis or in dead cells PI penetrates the membrane to stain nuclear DNA Combined Annexin V-Cy5 and PI staining allows distinction between cells at various stages of apoptosis and dead cells
ILEX LIFE SCIENCES LLC 2-MERCAPTOETHANOL 50MM 20ML
PAN-Biotech 2-Mercaptoethanol 50 mM in DPBS (20 ml). DESCRIPTION: 2-Mercaptoethanol, also known as beta-mercaptoethanol (BME), is a potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals. 2-Mercaptoethanol is not stable in solution, thus protocols generally require daily supplementation. PAN-Biotech 2-Mercaptoethanol is supplied at a concentration of 50 mM in Dulbecco's Phosphate Buffered Saline (DPBS). SOLUTION: 50 mM in DPBS. STORAGE: +2-8°C. STERILE: Yes. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
Apexbio Technology LLC Protease Inhibitor Cocktail (EDTA-Free 100X in DMSO) 20 X 1ml
Crude cell extracts typically contain numerous endogenous enzymes such as phosphatases and proteases that can degrade proteins During cell or tissue protein extraction in vitro protein synthesis is significantly inhibited while degradation is enhanced Adding inhibitors against these enzymes can improve yields of intact proteins Protease inhibitor cocktails are used with cell lysates or tissue extracts to enhance protein stability Our protease inhibitor cocktail has broad specificity and serves as a general-use protease inhibitor set suitable for purifying histidine-tagged proteins It contains multiple critical components including AEBSF Aprotinin Bestatin E-64 Leupeptin Phosphoramidon and Pepstatin A with broad inhibition specificity against serine proteases cysteine proteases acidic proteases aminopeptidases and metalloproteases The product is supplied as a ready-to-use solution in DMSO and can be used in WB Co-IP pull-down assays IF IHC and kinase assays
Crescent Chemical Co Inc MORPHOLINOETHANE SULFONIC ACID
Morpholinoethane sulfonic acid analytical grade pKa 20 = 6.15. Buffering substance for biochemistry and molecular biology (1). Physical constants (2). Size - 500G Storage Conditions - +15 °C TO +30 °C Catalog Number - 29834.04 CAS 4432-31-9