Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific Murashige & Skoog Medium with MES Buffer and Vitamins, 122.5 Grams of Powder, Makes 25 Liters of Solution
Murashige & Skoog Medium with MES Buffer and Vitamins (25 liters, 122.5 grams of powder) provides a high-volume solution for large-scale plant tissue culture operations. This formulation includes MES buffer, which maintains a stable pH and ensures optimal growth conditions for plant cells and tissues. The medium supports a variety of plant growth applications, including shoot multiplication, organogenesis, and regeneration. The addition of vitamins and essential nutrients promotes cell division and tissue development in controlled laboratory settings. This medium is ideal for research labs, agricultural facilities, and commercial propagation projects where large volumes of plant tissue culture medium are required for successful plant growth.
![Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659953.jpg-250.jpg)
Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], 500 G
PIPES (Piperazine N,N' bis(2 ethanesulfonic acid)) is a biochemical reagent commonly used to prepare buffer solutions that maintain a stable pH environment in various laboratory experiments. This 500g formulation is perfect for high-volume applications in research involving protein purification, enzyme assays, and microbiological cultures. PIPES provides reliable pH stability around 7.0 and is widely used in studies involving proteins, cells, and biological macromolecules. Its high buffering capacity makes it ideal for maintaining consistent experimental conditions during cell growth, enzymatic reactions, and protein analysis, ensuring accurate and reproducible results.
![Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660071.jpg-250.jpg)
Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 500 G
MES Monohydrate (2-(N-Morpholino) ethanesulfonic acid), available in a 500g volume, is a versatile biochemical reagent used for pH buffering in a variety of laboratory and research applications. This buffer is commonly used to stabilize the pH in enzyme assays, cell culture, and biochemical experiments. MES is especially useful in studies where precise pH control is crucial, such as in chromatography, electrophoresis, and protein purification. The 500g volume is ideal for labs requiring bulk quantities of MES for ongoing experiments or high-volume testing. MES ensures minimal interference with biological reactions, making it a reliable choice for many scientific studies in molecular biology and biochemistry.
![Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660241.jpg-250.jpg)
Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 250 G
HEPES, Sodium Salt is a biochemical reagent supplied in a 250g package, offering a reliable buffer for experiments requiring precise pH control. This sodium salt form of HEPES maintains a stable pH of 6.8–8.2, which is essential for a variety of biochemical and biological applications such as cell culture, protein analysis, and enzymatic assays. HEPES sodium salt is known for its excellent buffering capacity, ensuring that pH remains constant even during extended incubation periods. It is widely used in research and laboratory environments due to its low toxicity, making it a preferred choice for maintaining stable conditions during biological experiments.
![Alkali Scientific HEPPSO [N (2 Hydroxyethyl) piperazine N' (2 hydroxypropanesulfonic acid)], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661332.jpg-250.jpg)
Alkali Scientific HEPPSO [N (2 Hydroxyethyl) piperazine N' (2 hydroxypropanesulfonic acid)], 100 G
HEPPSO (N-(2 Hydroxyethyl) piperazine N'-(2 hydroxypropanesulfonic acid)) is a biochemical reagent supplied in a 100g bottle. This buffering agent is commonly used in laboratories for maintaining a stable pH, particularly in molecular biology and biochemistry applications. HEPPSO is widely used for enzyme assays, protein purification, and chromatography, as well as in the preparation of biological samples where precise pH control is necessary. This high-quality reagent ensures consistent results in experiments that require a stable and controlled acidic environment, especially in cell culture, electrophoresis, and other biological assays.
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 6.0, 100 mL
Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications.The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information:pKa (25 °C): 7.1 (Free acid)Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.Appearance: LiquidPH Value: 6.0
Bioworld ACES, 100 g
ACES, High Purity Grade N-[carbamoylmethyl]-2-Aminoethane-sulfonic acidpKa: (20°C): 6.8+0.1Appearance: White crystalsHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 6.1-7.5
Alkali Scientific HEPES Buffered Saline Solution 2X, 250 Milliliters
HEPES Buffered Saline Solution 2X is a highly stable buffered solution commonly used in biological research and laboratory applications. It is formulated with HEPES (4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid) to maintain a stable pH in biological systems. The 250mL bottle contains a 2X concentrated solution that can be diluted to the required working concentration. HEPES Buffered Saline is widely used in cell culture, protein studies, and molecular biology applications, providing a stable environment for maintaining cell viability and pH stability during experiments. This solution is ideal for biological assays, enzyme studies, and other sensitive applications requiring precise pH control.
![Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], USP, 5 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659782.jpg-250.jpg)
Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], USP, 5 Kg
PIPES [Piperazine N N' bis(2 ethanesulfonic acid)] is a biochemical buffer available in USP-grade, designed for a wide range of scientific and research applications. This 5kg container is ideal for laboratories that require large volumes of buffer solution for high-throughput processes, including protein studies, enzymatic assays, and cell culture applications. PIPES is known for its ability to maintain a stable pH under various conditions where other buffers may not be suitable. It is especially effective in high-salt and high-temperature environments. The 5kg pack ensures that large-scale laboratory projects can proceed without the need for frequent reordering, making it both a cost-effective and convenient solution.
AG Scientific Inc HEPPS 25 G
HEPPS is a biological buffer with a useful pH range of 7.3-8.7. pKa=8.0 at 25°C.
Electron Microscopy Sciences Pipes 1.5 Sodium Salt, Crystal, 6.8 @25°C pH 100 GR
(Piperazine-N,N-bis (2-ethanesulfonic acid,) 1.5 Sodium)
F.W. 335.3
pH: 6.8 @25°C