Biotin and derivatives
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Medchemexpress LLC DBCO-biotin | 1418217-95-4 | MFCD22380751 | 98.5% | 502.63 g·mol⁻1 | C28H30N4O3S | 10MG
DBCO-Biotin is a bifunctional click-chemistry reagent that links a dibenzocyclooctyne (DBCO) reactive moiety to a biotin affinity tag, enabling copper-free strain-promoted alkyne-azide cycloaddition (SPAAC) for bioconjugation and probe development. Supplied as a high-purity solid for laboratory research, it is commonly used in bioconjugation, affinity enrichment, and linker synthesis applications.
- Enables copper-free click chemistry via DBCO-mediated SPAAC.
- Provides a biotin tag for affinity capture and detection.
- High purity suitable for sensitive bioconjugation workflows.
- Available in small mg-scale quantities for research use.
- Stable when stored under recommended conditions; consult safety data for handling.
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Medchemexpress LLC PD-L1 Human Biotin 20ug
Recombinant, biotinylated human PD-L1 produced in HEK293 cells and supplied as a lyophilized 20 μg vial for research use. The protein includes C-terminal human Fc and Avi tags, is characterized for purity and molecular mass, and exhibits specific binding activity suitable for PD-1/PD-L1 interaction and immunoassay applications.
- Expressed in HEK293 mammalian cells.
- Contains C-terminal human Fc and Avi tags.
- Site-specific biotinylation via Avi tag.
- Purity greater than 95% by reducing SDS-PAGE.
- Lyophilized from 20 mM PB, 150 mM NaCl, pH 7.4.
- Predicted molecular mass 54.2 kDa; apparent 70-95 kDa by SDS-PAGE.
- Demonstrated binding activity with ED50 of 15.30 ng/mL.
- Store frozen and aliquot after reconstitution for long-term stability.