HPLC Columns
Waters Corp Waters XBridge BEH C18 Column, 5 μm, 4.6 mm X 30 mm
The XBridge BEH C18 sorbent serves as a workhorse for HPLC method development and can be utilized across a mobile phase pH range of 1 to 12. Its trifunctionally bonded C18 ligand offers a universal column suitable for most HPLC separations.
- Can be used over an entire range of mobile phase pH (1 to 12) as a workhorse for HPLC method development.
- Trifunctionally bonded C18 ligand provides a universal column for most HPLC separations.
- Offers the widest usable pH range.
- Provides superior low pH stability.
- Features ultra-low column bleed.
Waters Corp Waters XBridge BEH C8 Column, 130Å, 5 μm, 3 mm X 250 mm
The XBridge BEH C8 sorbent is less retentive compared to the XBridge BEH C18 sorbent. It is preferred for methods requiring faster analysis while improving peak shape and chromatographic performance. This trifunctionally bonded sorbent provides the widest usable pH range (1-12), superior low pH stability, and ultra-low column bleed.
- C8 chemistry
- Separation mode: Reversed phase
- Hybrid particle substrate
- pH range of 1 to 12
- Maximum pressure: 6000 psi (415 bar)
- Low silanol activity
- Spherical particle shape
- Particle size: 5 μm
- Pore size: 130 Å
- For HPLC systems
- BEH particle technology
- Inner diameter: 3 mm
- Length: 250 mm
- Carbon load: 13%
- 1 unit per package
Waters Corp Spherisorb Column, C8, 3 μm, 4.6 mm × 50 mm
These C8 columns are general purpose, silica-based reversed-phase columns that contain an Octyl ligand. They are USP L7 columns used when a less retentive sorbent compared to ODS columns is required.
- General purpose, silica-based reversed-phase column.
- Contains an Octyl ligand.
- Has a USP L7 classification.
- Offers less retention compared to ODS columns.
Waters Corp CORTECS Column, 90Å, 2.7 μm, 4.6 mm, 100 mm
These phenyl columns offer complementary selectivity to C18 stationary phases. Their particle morphology delivers high efficiency and lower backpressures compared to fully porous particles of similar size. They also provide ultra-low column MS bleed, long lifetimes, and excellent peak shape.
- Offers complementary selectivity to C18 stationary phases.
- Provides high efficiency and lower backpressures.
- Ensures ultra-low column MS bleed.
- Delivers long lifetimes and excellent peak shape.
- Features spherical solid core silica particles.
- Compatible with UPLC and UHPLC systems.
- Designed for a pH range of 2 to 8.
- Resistant to pressures up to 18000 psi (1240 Bar).
Waters Corp Waters XSelect HSS T3 Column, 100Å, 2.5 μm, 3 mm X 50 mm
The XSelect HSS T3 is a low-coverage C18 phase column compatible with 100% aqueous mobile phase, designed for separating polar and non-polar compounds. These 2.5 μm eXtended Performance (XP) columns provide exceptional separation performance, robustness, and high throughput for HPLC assays, with seamless transferability to UPLC separations.
- Chemistry: C18
- Separation mode: Reversed phase
- Particle substrate: Silica
- pH range: 2 to 8
- Temperature limits: 45°C
- Maximum pressure: 18000 psi (1240 Bar)
- Particle size: 2.5 μm
- Pore size: 100 Å
- Inner diameter: 3 mm
- Length: 50 mm
- System compatibility: UPLC, UHPLC
Waters Corp XBridge BEH HILIC XP Column, 130Å, 2.5 µm, 2.1 mm X 75 mm, 1/pk
XBridge BEH HILIC columns contain unbonded BEH substrate and are designed to retain and separate very polar compounds. These unique columns are optimized and tested to produce efficient, reproducible separations under HPLC HILIC conditions. More rugged than silica based HILIC phases, the BEH particle’s chemical stability results in long column lifetimes. Packed in ultra-low dispersion hardware, 2.5 µm eXtended Performance [XP] columns enable exceptional separation performance, robustness and throughput for HPLC assays while enabling a seamless transition path toward UPLC adoption.
Waters Corp ACQUITY - XBridge BEH Amide Method Transfer Kit, 130Å, 1.7 - 3.5 µm, 2.1 - 4.6 mm X 100 - 150mm, 2/pk
Method transfer kits are designed to preserve the integrity of a separation as it is transferred between UPLC and HPLC platforms. Based on the concept of maintaining column length [L] to particle size [dp] ratio [L/dp], these kits provide an ACQUITY UPLC column with an HPLC column of equivalent selectivity and resolving power. Using the ACQUITY UPLC columns calculator, methods can be fully transferred from HPLC to UPLC or from UPLC to HPLC.
Waters Corp Waters HPLC Column, Cyano, 3.5 μm, 3 mm x 75 mm
The XSelect HSS CN chemistry is an ultra-performance general purpose propyl cyano bonded phase, offering superior peak shape for bases, increased retention, and extended column lifetimes at low pH. These HPLC columns are fully scalable and transferable to ACQUITY UPLC separations.
- Superior peak shape for bases
- Increased retention and extended column lifetimes at low pH
- Chemistry: Cyano (CN)
- Separation mode: Reversed phase
- Particle substrate: Silica
- pH range: 2–8
- Maximum pressure: 6000 psi (415 Bar)
- Particle size: 3.5 μm
- Pore size: 100 Å
- Inner diameter: 3 mm
- Length: 75 mm
- Carbon load: 5%
- Ligand type: Monofunctional Cyano-Propyl
- USP classification: L10
- Compatible with normal-phase and reversed-phase separations
- Surface area: 230 m²/g
Waters Corporation Prep Column, C8, 5 μm, 7.8 mm, 50 mm
Symmetry columns continue to set the standard for HPLC drug assays, providing confidence in the long-term compliance of HPLC methods. They offer excellent peak shape, selectivity, column-to-column and batch-to-batch reproducibility, and other critical performance characteristics. Available in a variety of particle sizes, chemistries, and dimensions.
- C8 chemistry.
- Reversed phase separation mode.
- Particle substrate: Silica.
- pH range 2 to 8.
- Temperature limits: 45°C.
- Maximum pressure: 6000 psi (415 Bar).
- Particle size: 5 μm.
- Pore size: 100 Å.
- Inner diameter: 7.8 mm.
- Length: 50 mm.
- Endcapped.
- Low silanol activity.
- Spherical particle shape.
Waters Corp Waters Column, C18, 5 μm, 120 Å, 3 mm x 250 mm
Polyaromatic hydrocarbons (PAHs) are frequently monitored environmental contaminants. Standard and official methods for PAH analysis often specify HPLC, usually with UV and fluorescence detection, as the recommended analytical procedure. This column is designed for the HPLC analysis of PAHs.
- Allows for baseline resolution and exceptional peak symmetry for all target PAH analytes in a short time, using a simple water/acetonitrile mobile phase.
- Provides superior peak identification and quantitation for PAHs.
- Produced with ultra-pure reagents in a cGMP, ISO 9001-certified facility.
- Each batch of packing material is chromatographically tested with acidic, basic, and neutral analytes.
- Results are held to limited specification ranges to ensure good, repeatable performance.
Waters Corp XBridge BEH HILIC Column, 130Å, 5 µm, 4.6 mm X 30 mm, 1/pk
XBridge BEH HILIC columns contain unbonded BEH substrate and are designed to retain and separate very polar compounds. These unique columns are optimized and tested to produce efficient, reproducible separations under HPLC HILIC conditions. More rugged than silica based HILIC phases, the BEH particle’s chemical stability results in long column lifetimes.
Waters Corp Waters Column, C8, 3.5 μm, 100 Å, 3 mm x 50 mm
SunFire C8 columns are utilized for general purpose method development at low pH. The C8 ligand is favored for certain assays due to its reduced hydrophobicity, making it less retentive compared to SunFire C18.
- C8 chemistry
- Reversed phase separation mode
- Silica particle substrate
- pH range from 2 to 8
- Maximum pressure of 6000 psi (415 Bar)
- Endcapped
- Low silanol activity
- Spherical particle shape
- 3.5 μm particle size
- 100 Å pore size
- HPLC system compatible
- L7 USP classification
- 3 mm inner diameter
- 50 mm length
- 12% carbon load
- 1 unit per package
Waters Corp OBD Column, Shield RP8, 10 μm, 19 x 150 mm
These hybrid-based, reversed-phase C8 preparative columns utilize a first-generation hybrid particle (silica co-polymer) stationary phase. This combines silica's high efficiency with a polymer's expanded pH range, offering ruggedness, high mechanical strength, and an embedded polar group for enhanced peak shape with basic compounds.
- Embedded polar group technology delivers optimal peak shape and unique selectivity, ensuring excellent water wettability.
- Offers superior pH stability with a wider pH range (2 to 12) compared to silica-based columns, allowing greater selectivity choices and improved peak resolution.
- Provides high mechanical strength and efficiency due to hybrid particle technology.
- Achieves up to 60x increased mass loading compared to conventional silica-based materials.
- Facilitates easy scale-up from analytical to preparative chromatography.
Waters Corp Bondapak Radial-Pak Cartridge, C18, 125Å, 8 x 100 mm
Radial-Pak columns are plastic compression cartridge segments that insert into radial compression modules (RCM-holder sold separately) for use on liquid chromatography systems.
- C18 chemistry for reversed phase separation.
- Silica particle substrate.
- Operates within a pH range of 2 to 8.
- Temperature limit of 45°C.
- Maximum pressure of 1500 psi (100 Bar).
- Endcapped with high silanol activity.
- Irregular particle shape.
- Particle size of 37 to 55 μm.
- Pore size of 125 Å.
- Designed for HPLC preparative systems.
- Suitable for LC/MS and LC techniques.
- L1 USP classification.
- 8 mm inner diameter and 100 mm length.
- 14% carbon load.
Waters Corp Styragel HT 6 Column, 1,000,000 Å, 10 μm, 7.8 mm × 300 mm
Styragel HT columns are packed with 10 μm particles with a narrow particle size distribution, enhancing the packed-bed stability in the column. These rugged GPC columns deliver exceptional column lifetime in high-temperature GPC applications.
- Separation mode: SEC/GPC
- Particle substrate: Polymer
- pH range: 2 to 12
- Molecular weight range: 200,000 to 10,000,000
- Particle shape: Spherical
- Particle size: 10 μm
- Endfitting type: Waters
- Pore size: 1,000,000 Å
- Format: Column
- Packing solvent: THF
- System: HPLC
- Technique: GPC
- Inner diameter: 7.8 mm
- Length: 300 mm
- Units per package: 1 pk
- Hazardous material: This product contains hazardous material which requires special freight handling. Additional charges may apply.