Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (132,619)

Singleplex and Multiplex Bead Based Immunoassays (6,174)

ELISA Substrates and Reagents (2,341)

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466 – 480 of 138,521 results

466

Invitrogen™ Mouse IL-6 ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Interassay CV	6.7%
Intraassay CV	5.8%
Gene Symbol	Il6
Total Assay Time	3 hr 30 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity	<3 pg/mL
Storage Requirements	2-8°C
Assay Range	7.8-500 pg/mL
For Use With (Application)	Colorimetric
Test Time	1 hr 20 min
Accession Number	P08505
Regulatory Status	RUO
Target	IL-6
Kit Contents	Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision	<3 pg/mL
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	16193
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

467

Invitrogen™ Human Insulin ELISA Kit

ELISA

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
Protein Family	Hormones & Receptors
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Human
Conjugate	HRP
Interassay CV	8.5%
Intraassay CV	5.4%
Gene Symbol	INS
Total Assay Time	45 min
Assay Sensitivity	0.17 μIU/mL
Storage Requirements	2-8°C
Assay Range	5.1-250 μlU/mL
Test Time	1 hr 20 min
Accession Number	P01308
Regulatory Status	RUO
Target	Insulin
Kit Contents	Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Controls,Standard,Standard Dilution Buffer,Incubation Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Controls
Gene Alias	AA986540; IDDM; IDDM1; IDDM2; ILPR; INS; Ins1; Ins-1; Ins2; Ins-2; Ins2-rs1; InsII; Insulin; insulin 1; insulin 2; Insulin A chain; Insulin B chain; insulin I; insulin II; insulin-1; Insulin-1 A chain; Insulin-1 B chain; Insulin-2; Insulin-2 A chain; Insulin-2 B chain; IRDN; Mody; MODY10; Mody4; preproinsulin; proinsulinAA986540; IDDM; IDDM1; IDDM2; ILPR; INS; Ins1; Ins-1; Ins2; Ins-2; Ins2-rs1; InsII; Insulin; insulin 1; insulin 2; Insulin A chain; Insulin B chain; insulin I; insulin II; insulin-1; Insulin-1 A chain; Insulin-1 B chain; Insulin-2; Insulin-2 A chain; Insulin-2 B chain; IRDN; Mody; MODY10; Mody4; preproinsulin; proinsulin; IDDM; IDDM1; IDDM2; ILPR; IRDN; MODY10
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Gene ID (Entrez)	3630
Sample Type	Serum
Label or Dye	Biotin

468

Invitrogen™ IL-4 Mouse ProcartaPlex™ Simplex Kit

The Mouse IL-4 Simplex ProcartaPlex Kit measures IL-4 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation.

Pricing & Availability

469

Promotions Available

R&D Systems™ Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit

Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability
Specifications

Target	CCL2/JE/MCP-1
Specificity	Natural and Recombinant Mouse MCP-1
Host Species	Mouse
Gene ID (Entrez)	6347
Gene Symbol	CCL2
Total Assay Time	4.5 hr.
Sample Volume	Cell Culture Supernates (50 uL),Tissue Lysates (50 uL),Tissue Culture Supernates (50 uL),Serum (50 uL),EDTA Plasma (50 uL),Heparin Plasma (50 uL)
Assay Sensitivity	0.666 pg/mL
Storage Requirements	Store the unopened product at 2° to 8°C. Do not use past expiration date.
Assay Range	7.81 - 500 pg/mL
For Use With (Application)	ELISA

470

R&D Systems™ Mouse IFN-beta Quantikine ELISA Kit

Designed to measure mouse IFN-beta in cell culture supernates, serum, and plasma.

Pricing & Availability

471

Invitrogen™ C3a Human ProcartaPlex™ Simplex Kit

The Human C3a Simplex ProcartaPlex Kit measures C3a protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation.

Pricing & Availability

472

Invitrogen™ Human AMPK alpha-1,2 (Phospho) [pT172] ELISA Kit

ELISA

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
Protein Family	Kinases & Inhibitors
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Human,Mouse,Rat
Conjugate	HRP
Interassay CV	7.6%
Intraassay CV	4.8%
Substrate Type	Serine/Threonine Kinases
Gene Symbol	PRKAA1, PRKAA2
Total Assay Time	4 hr
Assay Sensitivity	<1 U/mL
Storage Requirements	2-8°C
Assay Range	< 1 U/mL
Test Time	1 hr 20 min
Accession Number	P54645, P54646, Q09137, Q13131, Q5EG47, Q8BRK8
Regulatory Status	RUO
Target	AMPK alpha-1,2
Kit Contents	Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Detection Antibody, anti-rabbit-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Standard Dilution Buffer,Detection Antibody,anti-rabbit-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Gene Alias	2310008I11Rik; 5'-AMP-activated protein kinase alpha-1 catalytic subunit; 5'-AMP-activated protein kinase alpha-2 catalytic subunit; 5'-AMP-activated protein kinase catalytic alpha-2 subunit; 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; 63 kDa subunit; A830082D05; AAPK2; ACACA kinase; acetyl-CoA carboxylase kinase; AI194361; AI450832; AL024255; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase; AMP-activated protein kinase alpha 2; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMP-activated protein kinase alpha-2 variant B; AMP-activated protein kinase, alpha 1 catalytic subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK; AMPK 63 kDa subunit; AMPK alpha 1; AMPK alpha 2; AMPK alpha-1 chain; AMPK alpha-2 chain; AMPK subunit alpha-1; AMPK subunit alpha-2; AMPK1; AMPK2; AMPKa1; AMPKa2; AMPK-activated protein kinase alpha-2 subunit; AMPKalpha1; AMPK-alpha-1 chain; AMPKalpha2; AMPK-alpha-2 chain; C130083N04Rik; HMGCR kinase; hydroxymethylglutaryl-CoA reductase kinase; pAMPK; PRKAA; Prkaa1; Prkaa2; protein kinase; protein kinase AMP-activated alpha 2 catalytic subunit; protein kinase AMP-activated catalytic subunit alpha 1; protein kinase AMP-activated catalytic subunit alpha 2; protein kinase, AMP-activated, alpha 1 catalytic subunit; protein kinase, AMP-activated, alpha 2 catalytic subunit; tau-protein kinase PRKAA12310008I11Rik; 5'-AMP-activated protein kinase alpha-1 catalytic subunit; 5'-AMP-activated protein kinase alpha-2 catalytic subunit; 5'-AMP-activated protein kinase catalytic alpha-2 subunit; 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; 63 kDa subunit; A830082D05; AAPK2; ACACA kinase; acetyl-CoA carboxylase kinase; AI194361; AI450832; AL024255; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase; AMP-activated protein kinase alpha 2; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMP-activated protein kinase alpha-2 variant B; AMP-activated protein kinase, alpha 1 catalytic subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK; AMPK 63 kDa subunit; AMPK alpha 1; AMPK alpha 2; AMPK alpha-1 chain; AMPK alpha-2 chain; AMPK subunit alpha-1; AMPK subunit alpha-2; AMPK1; AMPK2; AMPKa1; AMPKa2; AMPK-activated protein kinase alpha-2 subunit; AMPKalpha1; AMPK-alpha-1 chain; AMPKalpha2; AMPK-alpha-2 chain; C130083N04Rik; HMGCR kinase; hydroxymethylglutaryl-CoA reductase kinase; pAMPK; PRKAA; Prkaa1; Prkaa2; protein kinase; protein kinase AMP-activated alpha 2 catalytic subunit; protein kinase AMP-activated catalytic subunit alpha 1; protein kinase AMP-activated catalytic subunit alpha 2; protein kinase, AMP-activated, alpha 1 catalytic subunit; protein kinase, AMP-activated, alpha 2 catalytic subunit; tau-protein kinase PRKAA1; AMPK; AMPK2; AMPKa2; PRKAA;AMPK; AMPKa1
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Gene ID (Entrez)	105787, 108079, 5562, 5563, 65248, 78975
Sample Type	Cell Lysates
Label or Dye	HRP

473

Promotions Available

R&D Systems™ Human VEGF Quantikine ELISA Kit

Human VEGF Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability
Specifications

Specificity	Natural and Recombinant Human VEGF. This assay also recognizes Recombinant Human VEGF165b.
Target Species	Human
Conjugate	HRP
Gene ID (Entrez)	7422
Sample Type	Cell Culture Supernatant,Plasma (EDTA, Citrate, Heparin),Serum
Sample Volume	Cell Culture Supernates (200uL),Serum (100uL),EDTA Plasma (100uL),Heparin Plasma (100uL),Citrate Plasma (100uL)
Synonym	MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor
Assay Sensitivity	5 pg/mL (Cell Culture Supernates), 9 pg/mL (Serum, Heparin Plasma, Citrate Plasma, EDTA Plasma)
Storage Requirements	Store the unopened product at 2° to 8°C. Do not use past expiration date.
Assay Range	15.60 to 1000 pg/mL (Cell Culture Supernates), 31.30 to 2000 pg/mL (Serum, Heparin Plasma, Citrate Plasma, EDTA Plasma)
For Use With (Application)	ELISA
Cross Reactivity	Cross-species reactivity observed with 1 or more species tested. <0.5% cross-reactivity observed with available related molecules.

474

Invitrogen™ Tissue Extraction Reagent I

Lysis buffer for total protein extraction from tissue samples

Pricing & Availability

475

Invitrogen™ Mouse VEGF-A ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Interassay CV	6.4%
Intraassay CV	3.8%
Gene Symbol	VEGFA
Total Assay Time	4 hr 30 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	20.0 pg/mL
Storage Requirements	2-8°C
Assay Range	62.5-4000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Accession Number	Q00731
Regulatory Status	RUO
Target	VEGF
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	22339
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin
Synonym	Vascular endothelial growth factor A, VPF

476

Invitrogen™ Human Albumin ELISA Kit

ELISA

Pricing & Availability
Specifications

Protein Family	Other Proteins
Target Species	Human
Conjugate	HRP
Intraassay CV	<10%
Total Assay Time	4 hr 45 min
Sample Volume	Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
Research Discipline	Other Research
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hr 20 min
Assay	Solid-phase sandwich ELISA
Target	Human Serum Albumin
Specificity	This ELISA pair antibody detects human Albumin. Other species not determined.
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Label or Dye	Biotin
Calibration Range	60 pg/mL to 12,000 pg/mL
Interassay CV	<12%
Gene Symbol	ALB
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	6-1200 ng/mL
Accession Number	P02768
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	213
Sample Type	Plasma,Serum,Supernatant
Synonym	GIG20, ANALBA, FDAH, PRO0883, PRO0903, PRO1341

477

Invitrogen™ Human CD163 ELISA Kit

ELISA

Pricing & Availability
Specifications

Protein Family	Other Proteins
Target Species	Human
Conjugate	HRP
Intraassay CV	<10%
Total Assay Time	4 hr 45 min
Sample Volume	Plasma 2 μL, Serum 2 μL, Supernatant 100 μL
Assay Sensitivity	30 pg/mL
Research Discipline	Other Research
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hr 20 min
Assay	Solid-phase sandwich ELISA
Target	CD163
Specificity	In this sandwich immunoassay, no cross-reactivity with recombinant mouse CD163, rmCD6 or recombinant human CD6.
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Label or Dye	Biotin
Calibration Range	30 pg/mL to 8000 pg/mL
Interassay CV	<12%
Gene Symbol	CD163
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	30-8000 pg/mL
Accession Number	Q86VB7
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	9332
Sample Type	Plasma,Serum,Supernatant
Synonym	M130, MM130

478

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

Pricing & Availability
Specifications

Substrate Properties	Chemical Substrate
Product Type	ELISA Substrate
Substrate Type	HRP (Horseradish Peroxidase)
Detection Method	Colorimetric

479

Novus Biologicals™ Human Prothrombin Fragment 1+2 ELISA Kit (Colorimetric)

Colorimetric ELISA Kit

Pricing & Availability

480

Invitrogen™ Human IL-17A ProQuantum Immunoassay Kit

ELISA

Pricing & Availability
Specifications

Immunoassay Kit Format	Proximity-based Immunoassay
Target Species	Human
Conjugate	FAM
Incubation Time	1 hr.
Total Assay Time	2 hr
Sample Volume	Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Assay Sensitivity	<0.3 pg/mL
Storage Requirements	-20°C
Assay Range	0.32-25000 pg/mL
For Use With (Application)	Protein Quantitation
Test Time	30 min
Detection Method	Taqman™ Real-time PCR
Accession Number	Q16552
Regulatory Status	RUO
Target	IL-17A
Kit Contents	Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase
For Use With (Equipment)	qPCR Instruments
Product Type	ProQuantum
Gene ID (Entrez)	3605
Sample Type	Plasma,Serum,Supernatant
Label or Dye	FAM™

Immunoassay Reagents and Kits

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R&D Systems™ Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

R&D Systems™ Mouse IFN-beta Quantikine ELISA Kit GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

R&D Systems™ Human VEGF Quantikine ELISA Kit Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Novus Biologicals™ Human Prothrombin Fragment 1+2 ELISA Kit (Colorimetric) Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

Invitrogen™ Human IL-17A ProQuantum Immunoassay Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

R&D Systems™ Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit

R&D Systems™ Mouse IFN-beta Quantikine ELISA Kit

R&D Systems™ Human VEGF Quantikine ELISA Kit

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Novus Biologicals™ Human Prothrombin Fragment 1+2 ELISA Kit (Colorimetric)

Invitrogen™ Human IL-17A ProQuantum Immunoassay Kit