Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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Invitrogen™ Human Ferritin ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Protein Family | Other Proteins |
|---|---|
| Host Species | Human |
| Target Species | Human |
| Conjugate | HRP |
| Description | Ferritin ELISA Kit, 96 Assays, 1 Kit, Human Species, Other Proteins Protein Family, Inflammation Research Discipline, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Ferritin in Samples Using a Microplate Reader |
| Intraassay CV | <10% |
| Detectable Analytes | Ferritin |
| Substrate Type | TMB |
| Total Assay Time | 4 hr 45 min |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 100 μL |
| Assay Sensitivity | 600 pg/mL |
| Research Discipline | Inflammation |
| For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| Test Time | 1 hr 20 min |
| Detection Method | Colorimetric |
| Assay | Solid-phase sandwich ELISA |
| Target | Ferritin |
| Specificity | This Ferritin ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Label or Dye | Biotin |
| Calibration Range | 0.6 ng/mL to 200 ng/mL |
| Interassay CV | <12% |
| Gene Symbol | FTH1, FTL |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 0.6-200 ng/mL |
| Accession Number | P02792, P02794 |
| Regulatory Status | RUO |
| Product Type | ELISA |
| Gene ID (Entrez) | 2495, 2512 |
| Sample Type | Plasma,Serum,Supernatant |
| Synonym | LFTD, NBIA3 |
R&D Systems™ Mouse Total MMP-9 Quantikine ELISA Kit
Mouse Total MMP-9 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
Novus Biologicals™ Human HMGB1/HMG-1 ELISA Kit (Colorimetric)
ELISA kits are ideal for the quantification of target antigens in tissue extracts, serum and cell culture.
R&D Systems™ Human PDGF-BB Quantikine ELISA Kit
Human PDGF-BB Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
| Specificity | Natural and Recombinant Human PDGF-BB |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Product Type | Solid Phase Sandwich ELISA |
| Gene ID (Entrez) | 5155 |
| Sample Type | Cell Culture Supernatant,Platelet-poor Plasma (EDTA, Heparin),Serum |
| Sample Volume | Cell Culture Supernates (100uL),Serum (10uL),Platelet-poor EDTA Plasma (100uL),Platelet-poor Heparin Plasma (100uL) |
| Assay Sensitivity | 15pg/mL |
| Storage Requirements | Store the unopened product at 2° to 8°C. Do not use past expiration date. |
| Assay Range | 31.20 - 2000pg/mL |
| For Use With (Application) | ELISA |
| Cross Reactivity | <50% cross-species reactivity observed with species tested. Cross-reactivity observed with 1 or more available related molecules |
Invitrogen™ 3-Nitrotyrosine Competitive ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet or Dry Ice |
|---|---|
| Immunoassay Kit Format | Sandwich ELISA Kit |
| Target Species | All Species |
| Conjugate | HRP |
| Interassay CV | <10% |
| Intraassay CV | <10% |
| Total Assay Time | 2 hr 30 min |
| Assay Sensitivity | 0.94 ng/mL |
| Storage Requirements | Refer to product documentation for component specific storage temperature. |
| Assay Range | 1.56-100 ng/mL |
| Test Time | 1 hr 20 min |
| Regulatory Status | RUO |
| Target | 3-Nitrotyrosine |
| Kit Contents | Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer,Standard,Biotinylated Detection Antibody,HRP Conjugate,Standard & Sample Diluent,Biotinylated Detection Antibody Diluent,HRP Conjugate Diluent,Wash Buffer,TMB Substrate,Stop Solution,Plate Sealer |
| Gene Alias | 3-Nitro-tyrosine; C9H10N2O5; Nitrotyrosine3-Nitro-tyrosine; C9H10N2O5; Nitrotyrosine; |
| Product Type | ELISA |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Sample Type | Plasma,Serum |
| Label or Dye | Biotin |
R&D Systems™ Human CCL22/MDC Quantikine ELISA Kit
Human CCL22/MDC Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
Invitrogen™ Canine IL-12 p40 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10% |
| Gene Symbol | Il12b |
| Total Assay Time | 4 hr 45 min |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 24 pg/mL |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 24.58-6000 pg/mL |
| Test Time | 1 hr 20 min |
| Accession Number | Q28268 |
| Regulatory Status | RUO |
| Target | IL-12 p40 |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 403976 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
Novus Biologicals™ Human Apolipoprotein B100/ApoB100 ELISA Kit (Colorimetric)
Colorimetric Assay Kit
| Assay Sensitivity | 37.5 ng/mL |
|---|---|
| Assay Range | 62.5 to 4000 ng/mL |
R&D Systems™ Human IL-4 Quantikine HS ELISA Kit
Human IL-4 Quantikine HS ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.
| Specificity | Natural and Recombinant Human IL-4 |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Product Type | Solid Phase Sandwich ELISA |
| Gene ID (Entrez) | 3565 |
| Sample Type | Cell Culture Supernatant,Serum |
| Sample Volume | Cell Culture Supernates (200uL),Serum (200uL) |
| Synonym | B cell growth factor 1, BCDF, B-cell stimulatory factor 1, BCGF1, BCGF-1, binetrakin, BSF1, BSF-1, IL4, IL-4 B_cell stimulatory factor 1, IL4E12, interleukin 4, interleukin-4, Lymphocyte stimulatory factor 1, MGC79402, Pitrakinra |
| Assay Sensitivity | 0.22pg/mL |
| Storage Requirements | Store the unopened product at 2° to 8°C. Do not use past expiration date. |
| Assay Range | 0.25 - 16pg/mL |
| For Use With (Application) | ELISA |
| Cross Reactivity | <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules. |
Invitrogen™ Human VEGF ProQuantum Immunoassay Kit
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Immunoassay Kit Format | Proximity-based Immunoassay |
|---|---|
| Target Species | Human |
| Conjugate | FAM |
| Incubation Time | 1 hr. |
| Total Assay Time | 2 hr |
| Sample Volume | Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL |
| Assay Sensitivity | <0.3 pg/mL |
| Storage Requirements | -20°C |
| Assay Range | 0.32-25000 pg/mL |
| For Use With (Application) | Protein Quantitation |
| Test Time | 30 min |
| Detection Method | Taqman™ Real-time PCR |
| Accession Number | P15692 |
| Regulatory Status | RUO |
| Target | VEGF |
| Kit Contents | Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase |
| For Use With (Equipment) | qPCR Instruments |
| Product Type | ProQuantum |
| Gene ID (Entrez) | 7422 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | FAM™ |
Invitrogen™ Mouse FSH ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet or Dry Ice |
|---|---|
| Protein Family | Glycoprotein hormone |
| Immunoassay Kit Format | Sandwich ELISA Kit |
| Target Species | Mouse |
| Conjugate | HRP |
| Interassay CV | <10% |
| Intraassay CV | <10% |
| Gene Symbol | CGA, FSHB |
| Total Assay Time | 3 hr 30 min |
| Assay Sensitivity | 0.94 ng/mL |
| Storage Requirements | Refer to product documentation for component specific storage temperature. |
| Assay Range | 1.56-100 ng/mL |
| Test Time | 1 hr 20 min |
| Accession Number | P01216, Q60687 |
| Regulatory Status | RUO |
| Target | Follicle Stimulating Hormone |
| Kit Contents | Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer,Standard,Biotinylated Detection Antibody,HRP Conjugate,Standard & Sample Diluent,Biotinylated Detection Antibody Diluent,HRP Conjugate Diluent,Wash Buffer,TMB Substrate,Stop Solution,Plate Sealer |
| Gene Alias | class 1 follicle-stimulating hormone beta polypeptide; class 2 follicle-stimulating hormone beta polypeptide; follicle - stimulating hormone subunit beta; follicle stimulating hormone beta; follicle stimulating hormone beta subunit; follicle stimulating hormone beta-subunit precursor; follicle stimulating hormone subunit beta; follicle stimulating hormone, beta polypeptide; follicle-stimulating hormone beta subunit; follicle-stimulating hormone beta-subunit; follicle-stimulating hormone subunit beta; follicule stimulating hormone, beta polypeptide; follitropin beta chain; Follitropin subunit beta; follitropin, beta chain; FSH; FSH-alpha; FSHB; FSH-B; Fshbeta; FSH-beta; HH24; LSH-alpha; prefollicle stimulating hormone beta chain; TSH-alphaclass 1 follicle-stimulating hormone beta polypeptide; class 2 follicle-stimulating hormone beta polypeptide; follicle - stimulating hormone subunit beta; follicle stimulating hormone beta; follicle stimulating hormone beta subunit; follicle stimulating hormone beta-subunit precursor; follicle stimulating hormone subunit beta; follicle stimulating hormone, beta polypeptide; follicle-stimulating hormone beta subunit; follicle-stimulating hormone beta-subunit; follicle-stimulating hormone subunit beta; follicule stimulating hormone, beta polypeptide; follitropin beta chain; Follitropin subunit beta; follitropin, beta chain; FSH; FSH-alpha; FSHB; FSH-B; Fshbeta; FSH-beta; HH24; LSH-alpha; prefollicle stimulating hormone beta chain; TSH-alpha; |
| Product Type | ELISA |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Gene ID (Entrez) | 12640, 14308 |
| Sample Type | Plasma,Serum |
| Label or Dye | Biotin |
Invitrogen™ Human HSP27 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 4.3% |
| Intraassay CV | 5.8% |
| Gene Symbol | HSPB1 |
| Total Assay Time | 3 hr 30 min |
| Sample Volume | Cell Lysate 10 μL, Plasma 10 μL, Serum 10 μL, Supernatant 10 μL |
| Assay Sensitivity | 10.9 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 15.6-1000 pg/mL |
| Test Time | 1 hr 20 min |
| Accession Number | P04792 |
| Regulatory Status | RUO |
| Target | HSP27 |
| Kit Contents | Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Controls,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 3315 |
| Sample Type | Cell Lysates,Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
Invitrogen™ Mouse Insulin ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Protein Family | Hormones & Receptors |
|---|---|
| Host Species | Mouse |
| Target Species | Mouse |
| Conjugate | HRP |
| Description | Insulin ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Hormones and Receptors Protein Family, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Insulin in Samples |
| Intraassay CV | <10% |
| Detectable Analytes | Mouse, human, rat, porcine and bovine insulin |
| Total Assay Time | 4 hr 45 min |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 100 μL |
| Research Discipline | Endocrinology |
| For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| Test Time | 1 hr 20 min |
| Detection Method | Colorimetric |
| Assay | Solid-phase sandwich ELISA |
| Target | Insulin |
| Specificity | This ELISA pair antibody detects mouse, human, rat, porcine and bovine Insulin. |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Label or Dye | Biotin |
| Interassay CV | <12% |
| Gene Symbol | Ins1, Ins2 |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 5-400 μIU/mL |
| Accession Number | P01325, P01326 |
| Regulatory Status | RUO |
| Product Type | ELISA |
| Gene ID (Entrez) | 16333, 16334 |
| Sample Type | Plasma,Serum,Supernatant |
| Synonym | Ins-1, Ins2-rs1 |