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Buffer and diluent solutions to prepare, dilute, and deliver protein and nucleic acid samples to electrophoresis gels for separation and analysis; includes solutions to stabilize, control the concentration of, denature, and reduce sample proteins.
Developed for use with the InstantOne ELISA Cell Signaling assays. The InstantOne ELISA Wash Buffer is supplied as a 10x concentrate and is used for washing the InstantOne ELISA cell signaling assay microplate. It is a simple mix of buffers, salts and mild detergent.
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Cell lysis buffer for WB and IP is a solution widely utilized for rapid lysis of cells and tissues under non-denaturing conditions to release intracellular proteins while preserving protein-protein interactions The buffer contains 20 mM Tris (pH 7 5) 150 mM NaCl and 1% Triton X-100 supplemented with sodium pyrophosphate -glycerophosphate EDTA sodium orthovanadate (Na VO ) and protease inhibitor leupeptin to prevent proteolysis and dephosphorylation Resulting protein extracts are suitable for downstream analyses including Western blotting immunoprecipitation co-immunoprecipitation ELISA and PAGE assays across animal plant fungal or bacterial samples
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Small and Specialty Supplier Partner Small and/or specialty supplier based on Federal laws and SBA requirements. Learn More
Red Blood Cell Lysis Buffer is a reagent used to selectively lyse non-nucleated erythrocytes It is designed to remove erythrocyte contamination from biological samples thereby enabling the purification of nucleated cells such as lymphocytes Red Blood Cell Lysis Buffer exerts its biological activity primarily through ammonium chloride-based osmotic shock which disrupts erythrocyte membranes while sparing nucleated cells Following red blood cell lysis the resulting preparations typically contain greater than 90% nucleated cells Based on these properties Red Blood Cell Lysis Buffer is utilized in applications such as nucleic acid extraction protein isolation cell culture assays and flow cytometry analysis of samples from human mouse rat and other mammalian sources
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