ELISA Substrates and Reagents
AdipoGen TMB dihydrochloride
Chemical. CAS 64285-73-0. Formula C16H20N2 . 2HCl. MW 240.3 . 72.9. Synthetic TMBZ is a chromogenic reagent utilized for peroxidase detection. It has been developed as an alternative to benzidine. Although the TMBZ solution is colorless, it turns bluish-green lambdamax 655 nm in the presence of hydrogen peroxide and peroxidase. The structure of this bluish-green complex is thought to be a radical form of two oxidized TMBZ molecules. This peroxidase substrate is used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays ELISA. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2M H2SO4 resulting in a yellow color, and read at 450nm.
AdipoGen TMB dihydrochloride
Chemical. CAS 64285-73-0. Formula C16H20N2 . 2HCl. MW 240.3 . 72.9. Synthetic TMBZ is a chromogenic reagent utilized for peroxidase detection. It has been developed as an alternative to benzidine. Although the TMBZ solution is colorless, it turns bluish-green lambdamax 655 nm in the presence of hydrogen peroxide and peroxidase. The structure of this bluish-green complex is thought to be a radical form of two oxidized TMBZ molecules. This peroxidase substrate is used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays ELISA. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2M H2SO4 resulting in a yellow color, and read at 450nm.
Bioss Acetyl CoA carboxylase Monoclo
Acetyl CoA carboxylase Primary Antibody Unconjugated Host Species Rabbit Target Species Human Mouse Rat Clonality Monoclonal Applications WB IHC-P IHC-F IF RecombinantConcentration 1ug/ul Size 100 ul Storage Buffer 10mM phosphate buffered saline(pH 7 4) with 150mM sodium chloride 0 05% BSA 0 02% sodium azide and 50% glycerol
Sigma Aldrich Fine Chemicals Biosciences SIGMAFAST™ OPD | 5 sets
SIGMAFAST™ OPD (o-Phenylenediamine dihydrochloride) tablets have been developed for use as a soluble substrate for the detection of peroxidase activity in enzyme immunoassays (EIA). OPD is the EIA substrate of choice as it exhibits high sensitivity. EIA applications utilizing OPD may be read in timed assays or stopped with dilute acid solutions for delayed readings.
Cell Signaling Technology PathScanR Phospho-HSP27 Ser82 Sandwich ELISA Kit 50 Kit
PathScanR Phospho-HSP27 Ser82 Sandwich ELISA Kit 50 Kit
Cell Signaling Technology PathScanR Phospho-HSP27 Ser82 Sandwich ELISA Kit 5 Kit
PathScanR Phospho-HSP27 Ser82 Sandwich ELISA Kit 5 Kit
ABclonal Technology Phospho-BRD4-T204 Rabbit pAb
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15,19)(q13,p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
ACROBiosystems CHO/Human IL-1 RAcP Stable Cell Line (CCHO-ATP257) - 2 vials, ≥5 x 10^6 cells per vial, frozen
CHO/Human IL-1 RAcP Stable Cell Line is an adherent CHO-derived cell line engineered to express full-length human IL-1 RAcP. Supplied frozen in cryopreserved vials, the line is QC-verified for surface expression and validated for passage stability to support reproducible cell-based binding assays and bioassay development.
- Provided as frozen vials with ≥5 x 10^6 cells per vial.
- Surface expression confirmed by flow cytometry.
- Suited for cell-based IL-1 RAcP binding and bioassay development.
- Adherent CHO cell line with Zeocin selection (200 μg/mL).
- Mycoplasma- and sterility-tested negative.
- Validated passage stability and full traceable QC records.
- Growth medium: F-12K + 10% FBS; freeze medium: serum-free cryopreservation medium.
Cell Signaling Technology PathScanR Phospho-c-Kit Tyr703 Sandwich ELISA Kit 50 Kit
PathScanR Phospho-c-Kit Tyr703 Sandwich ELISA Kit 50 Kit
ABclonal Technology Phospho-eIF2α-S51 Rabbit pAb
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2, MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).
ABclonal Technology Phospho-ErbB4/HER4-Y1284 Rabbit pAb
This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described, however, not all variants have been fully characterized.