Gel Electrophoresis Reagents and Buffers
ABclonal Technology INPPL1 Rabbit pAb
The protein encoded by this gene is an SH2-containing 5-inositol phosphatase that is involved in the regulation of insulin function. The encoded protein also plays a role in the regulation of epidermal growth factor receptor turnover and actin remodelling. Additionally, this gene supports metastatic growth in breast cancer and is a valuable biomarker for breast cancer.
Biotium DNAzure Blue Nucleic Acid Gel Stain, 100X
DNAzure Blue Gel Stain allows you to visualize DNA bands in gels by eye, with detection sensitivity rivaling most fluorescence-based gel stains. Key to the technology is a DNA-binding dye that turns from colorless to deep blue upon exposure to light. The benefits include: deep blue bands visible by the naked eye following 5-30 min. light exposure, ultrasensitive detection (as little as ~1 ng DNA), and simplified DNA band excision without DNA-damaging UV Light.
Electron Microscopy Sciences Millonig's Phosphate Buffer, 0.2M, 7.4 pH 500 ML
The pH of this buffer is 7.4 (0.1M); the osmolarity is 440 mosmols, so this buffer is hypertonic to most body fluids. Millonig recommended to use this buffer for the fixation of very hydrated tissues with osmium tetroxide. For marine organisms, a higher concentration of sodium chloride (3%) is recommended. We supply it in a double strength (0.2M), pH 7.4. Dilution is needed before use.
pH: 7.4
Electron Microscopy Sciences Acetate Buffer, 0.2M, pH 3.6-5.6 500 ML
Acetic Acid and Sodium Acetate, 0.2M, pH range 3.6-5.6. Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged. Desired pH should be specified when ordering.
pH: 3.6-5.6
Electron Microscopy Sciences Sorensens Phosphate Buffer, 0.2M, 1 L
A Phosphate buffered mixed salt prepared from sodium phosphate monobasic and sodium phosphate dibasic, pH 7.2.
Electron Microscopy Sciences Agarose SFR™ Agarose S FR White, Fine Powder 100 GR
CAS #9012-36-6 (C₆H₁₀O₅·C₆H₈O₄)n
Melting point: ≤ 70°C
Agarose SFR is a high resolution sieving agarose with unsurpassed clarity. DNA bands differing in size by 2% can be resolved in the range of 200 – 1,000 bp. This agarose is suitable for the analysis of AFLP's (Amplified Fragment Length Polymorphisms), STR's (Short Tandem Repeats) and tetranucleotide repeats. The low melting point of agarose SFR makes it an excellent medium for analytical and preparative electrophoresis.
Quality Biological Inc EDTA (0.5 M), pH 8.0, PK 4 X 100 mL
EDTA (Ethylenediaminetetraacetic acid), a metal chelator, is widely used in molecular biology as a chemical reagent to reduce nuclease activity (i.e. metalloenzyme). ¿ EDTA solution can be used to study cell biology, molecular biology, bioactive small molecules, biochemicals, solutions and reagents, inorganic salts, and chelators. ¿ Applications: Typically, EDTA is added to Tris base in order to prepare Tris EDTA (TE), which can be used to reconstitute nucleic acids (i.e. DNA). ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase and Protease tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Quality Biological Inc TBS 10X pH 7.4, 500 mL
TBS 10X pH 7.4 is used as a washing buffer for cellular and molecular biology techniques. ¿ DNase, RNase and Protease tested. ¿ Applications: TBS 10X pH 7.4 will be useful as a washing buffer for electrodes, microtiter plates, and alkaline phophatase or peroxidase conjugates. TBS 10X pH 7.4 can also be used in detergent preparation and Western blot or ELISA applications. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase, and Protease Tested ¿ pH: 7.4¿0.1 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Quality Biological Inc SDS 10%, 500 mL
Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. ¿ SDS is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. ¿ Applications: Especially useful for denaturing polyacrylamide gel electrophoresis (SDS-PAGE) ¿ Common component of cell lysis buffers ¿ Storage Conditions: 25¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase, and Protease Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Quality Biological Inc EDTA (0.5 M), pH 8.0, 500 mL
EDTA (Ethylenediaminetetraacetic acid), a metal chelator, is widely used in molecular biology as a chemical reagent to reduce nuclease activity (i.e. metalloenzyme). ¿ EDTA solution can be used to study cell biology, molecular biology, bioactive small molecules, biochemicals, solutions and reagents, inorganic salts, and chelators. ¿ Applications: Typically, EDTA is added to Tris base in order to prepare Tris EDTA (TE), which can be used to reconstitute nucleic acids (i.e. DNA). ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase and Protease tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Quality Biological Inc SDS 10%, PK 4 X 100 mL
Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. ¿ SDS is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. ¿ Applications: Especially useful for denaturing polyacrylamide gel electrophoresis (SDS-PAGE) ¿ Common component of cell lysis buffers ¿ Storage Conditions: 25¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase, and Protease Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Quality Biological Inc RNA Gel Buffer 10X (MOPS Buffer), 500 mL
MOPS Buffer is used at 1X for the electrophoresis of RNA as the running buffer to separate RNA samples on agarose and formaldehyde-agarose (denaturing) gels. ¿ MOPS buffer is also used for Northern Blots -the hybridization of total RNA or mRNA to a membrane. ¿ Liquid, 10X concentration ¿ Filtered through a 0.22 ¿m membrane ¿ DNase, RNase Tested ¿ Applications: MOPS Buffer is suitable for use in RNA Gel Electrophoresis, Northern Blot Analysis, and when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase Tested ¿ pH: 7.0¿ 0.1 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
New England Biolabs, Inc. DNase I Reaction Buffer – 6 ml
New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
