This monoclonal antibody provides highly specific detection of the 6xHIS affinity tag, widely used in recombinant protein expression, purification, and characterization. Clone 7B8 delivers strong affinity and low background in ELISA, Western blotting, immunoprecipitation, and protein‑tracking workflows. Its clean, reproducible signal enables precise monitoring of HIS‑tagged proteins in cell lysates, culture supernatants, and purified preparations. MHIS‑45A is ideal for molecular biology, protein engineering, and assay development requiring reliable HIS‑tag detection across diverse experimental formats.