Hydroxybenzoic Acid Derivatives
Apexbio Technology LLC HotStart Universal 2X Green qPCR Master Mix 25ml
Our product HotStart Universal 2 SYBR Green qPCR Master Mix has superior specificity robust amplification efficiency excellent reproducibility and high stability for the quantification of target DNA or cDNA This ready-to-use 2 premix formulation includes a novel antibody-mediated hot-start Taq DNA polymerase which along with the optimized buffer system ensures enhanced specificity and efficient amplification The SYBR Green I dye in the mix intercalates specifically into the minor groove regions of double-stranded DNA and emits green fluorescence upon binding to amplified DNA in each cycle enabling real-time indirect quantification of amplification products through fluorescence detection on qPCR instrumentation This reagent includes a specially formulated Specific ROX Reference Dye compatible with all qPCR instruments no need for adjusting ROX concentrations across different instruments Users only need to add primers and templates when preparing reaction mixtures
Apexbio Technology LLC HotStart Universal 2X Green qPCR Master Mix 50ml
Our product HotStart Universal 2 SYBR Green qPCR Master Mix has superior specificity robust amplification efficiency excellent reproducibility and high stability for the quantification of target DNA or cDNA This ready-to-use 2 premix formulation includes a novel antibody-mediated hot-start Taq DNA polymerase which along with the optimized buffer system ensures enhanced specificity and efficient amplification The SYBR Green I dye in the mix intercalates specifically into the minor groove regions of double-stranded DNA and emits green fluorescence upon binding to amplified DNA in each cycle enabling real-time indirect quantification of amplification products through fluorescence detection on qPCR instrumentation This reagent includes a specially formulated Specific ROX Reference Dye compatible with all qPCR instruments no need for adjusting ROX concentrations across different instruments Users only need to add primers and templates when preparing reaction mixtures
Apexbio Technology LLC Proteinase K 5ml
Proteinase K is a broad-spectrum serine protease Our product is derived from recombinant Pichia pastoris strains expressing the endoproteinase gene originally sourced from the fungus Tritirachium album limber It is a highly active enzyme widely used in the hydrolysis of a variety of proteins and enzymatic contaminants (including endonucleases exonucleases DNases and RNases) Proteinase K is therefore commonly used for DNA preparations as it does not impair DNA integrity Proteinase K preferentially cleaves peptide bonds adjacent to the carboxyl end of hydrophobic amino acids (aliphatic aromatic etc ) and exhibits excellent performance under various conditions such as different pH values buffer types detergents (e g SDS) chelating agents (e g EDTA) and temperatures Proteinase K is also used for enzyme mapping and the removal of unwanted enzymes from DNA preparations to enhance cloning efficiency The suitable working concentration usually ranges from 0 05 to 1 mg/mL