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Chemical solutions designed to preserve biological specimens by preventing autolysis and putrefaction, inhibiting bacterial and fungal growth, and making specimens resistant to damage during processing; ideal for a variety of fixation processes.
A modification of Bouin's solution. It is stable and will decalcify small bone specimens. Tissue that is fixed with Hollande's can be stained successfully with most stains, and the cupric acetate in the solution stabilizes red blood cell membranes and cosinophil and endocrine cell granules so that less lysis occurs than with Bouin's solution. Hollande's is widely used as a fixative for biopsy specimens of the gastrointestinal tract.
Thorough washing of the fixative prior to placing the specimen in a phosphate buffered formalin solution is necessary because the salts present in the solution will form an insoluble phosphate precipitate. Packaged in leak-proof histo-containers; prefilled ready-to-use. Cup: 15 ml Fill: 10 ml
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In SituT Fixative is utilized for routine, manual or automated tissue fixation. Cellular material or tissues can be immersed in In Situ for transport and preservation of antigenic sites. In Situ maintains the morphological integrity of cells or tissues, and enhances specific immunohistochermical staining in tissues embedded in paraffin medium.• In Situ permits in situ PCR reactions.• A nonhazardous tissue fixative exempt from EPA disposal regulations.• Virtually odorless.• Inactivates the HIV-1 virus in culture.• Maintains cellular morphology.• Optimizes immunohistochemistry results.
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Stains for hematologic elements. See reference Williams, W.j., Beutler, E., Erslev, A.J. and Rundles, R.W. Hematology, 2nd Ed. McGraw-Hill, YY, c 1977, pp 1627-1628.
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Paraformaldehyde solution is a neutral fixative solution used to fix tissues and cells in immunohistochemistry and immunocytochemistry protocols. Often stabilized in a phosphate buffer for delivery, this reagent actively cross-links proteins and DNA, immobilizing cellular components and maintaining the integrity of cell morphology. This reagent supports the accurate visualization of cells and tissues under a microscope, ensuring that researchers can observe fine details without degradation.
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