Thermal Cycler Accessories
Cayman Chemical GTS-21hydrochlorIde 10mg
A fluorogenic dye used as a long-term cell tracer ex/em spectra 577/602 nm respectively retained through cell division remains fluorescent for at least 72 hours
Bio Rad Laboratories DG32 Automated Droplet Generator Cartridges
DG32 CARTRIDG, QX100/QX200 DG. (Encompass)
Montana Molecular Green fMP-targeted cADDis cAMP Assay - 10mL
Measure real-time cAMP signaling kinetics with this flag-tagged MyrPalm cADDis cAMP sensor. Add fMP-tagged cADDis to your cells and measure robust signals the next day on a fluorescence imaging system. Decreasing green fluorescence indicates cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). Other Montana Molecular tools including BacMam-packaged GPCRs, red fluorescent membrane-targeted or cytosolic cAMP sensors, or red fluorescent sensors for DAG or Calcium can easily be co-expressed with this assay.