PCR Supplies
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Invitrogen™ TaqMan™ Cells-to-CT™ HepatoExpress™ Kit
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The TaqMan Cells-to-CT HepatoExpress Kit is a complete cell lysate system designed for gene expression analysis directly from hepatocytes without the need of RNA purification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Frozen, Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Form | Liquid |
| Isolation Technology | Direct Cell Lysis |
| For Use With (Application) | Gene Expression |
| High-throughput Compatibility | Yes |
| PCR Method | 2-step RT-qPCR |
| Detection Method | TaqMan |
| Target | cDNA |
| Format | Kit |
| Product Type | Direct Cell Lysis to RT-qPCR Kit |
| Sample Type | Hepatocytes, HepaRG, HepG2 |
| Reaction Speed | 10 min. |
| Label or Dye | ROX |
| Purification Target | RNA |
| Starting Material | Up to 100,000 Cultured Cells |
Applied Biosystems™ TaqPath™ BactoPure™ Microbial Detection Master Mix, no Rox
TaqPath™ BactoPure™ Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors. Does not contain a passive reference, recommended for use with assays utilizing JUN dye or higher order multiplexing (> three targets).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| For Use With (Application) | Microbial Detection |
|---|---|
| PCR Method | qPCR |
| Multiplex Capability | Up to 4-plex |
| Detection Method | Primer-probe |
| Includes | dUTP, UNG/UDG |
| Thermostability | 72 hr., Assembled Reaction |
| Sensitivity | Down to 1 copy |
| Passive Reference Dye | None |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 12k Flex Dx, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudio 6 Flex, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiA 7 System, ViiA 7 Dx, QuantStudio Dx, StepOnePlus System, 7500 Fast Dx System, StepOne System, Roche LightCycler PRO System, Bio-Rad CFX Opus 96 Real-Time PCR System |
| Sample Type | DNA |
| Reaction Speed | Fast or Standard |
| Inhibitor Tolerance (Simple) | Very High |
| Target Specificity | cDNA or gDNA only |
| Product Line | TaqPath |
Applied Biosystems™ TrueMark™ Comprehensive Microbiota Control
The TrueMark Comprehensive Microbiota Control contains a linearized multi-target plasmid pool with microbial target sequences commonly used on Custom OpenArray panels. The control has targets relevant to many microbial profiling applications, including respiratory tract, urinary tract, vaginal microbiota, and antibiotic resistance research.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry ice |
|---|---|
| Format | Tube |
| Shelf Life | 2 years |
| Product Line | TrueMark |
| Detection Method | Primer-probe |
Invitrogen™ SuperScript™ IV Template Switching RT Master Mix
Invitrogen SuperScript IV Template-Switching RT Master Mix is designed for high template switching efficiency in cDNA synthesis reactions.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Format | Master Mix |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Technique | Template Switching Reverse Transcription |
| Concentration | 4X |
| Starting Material | Cells or RNA |
Applied Biosystems™ TaqPath™ ProAmp™ Multiplex Master Mix
TaqPath ProAmp Multiplex Master Mix is for higher multiplexing (up to four targets) in high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet Ice |
|---|---|
| Content And Storage | Store at 2°C to 8°C in the dark. |
| For Use With (Application) | Genotyping,Pharmacogenomics |
| PCR Method | qPCR |
| Multiplex Capability | Up to 4-plex |
| Detection Method | Primer-probe |
| Includes | dUTP, UNG/UDG |
| Thermostability | 72 hr., Assembled Reaction |
| Polymerase | Taq DNA Polymerase |
| Product Type | qPCR Master Mix |
| Passive Reference Dye | Mustang Purple |
| For Use With (Equipment) | QuantStudio 3 or 5, QuantStudio 6/QuantStudio 7 Flex, QuantStudio 12K Flex, QuantStudio Dx, ViiA 7 Real-Time PCR System ViiA 7 Dx, 7500 Fast, StepOne or StepOnePlus |
| Reaction Speed | Fast or Standard |
| Inhibitor Tolerance (Simple) | Very High |
| Target Specificity | cDNA or gDNA only |
| Product Line | TaqPath |
Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Format | Tube |
| Product Type | 1-Step qPCR kit |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Sample Type | Mammalian Cells |
| Reaction Speed | 7 min. |
| Reagent Type | Cell Lysis |
| Starting Material | 10-100,000 Cells |
| For Use With (Application) | Gene Expression |
| PCR Method | 1-step RT-qPCR |
| Product Line | Cells-to-CT™, Power SYBR™ Green |
| Detection Method | SYBR |
Thermo Scientific™ Phusion™ HF and GC Buffer Packs
5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.
| Quantity | 4 x 1.5 mL |
|---|---|
| For Use With (Application) | Standard PCR |
TaqMan™ PGx Express Panel, 384-well plate
This TaqMan PGx (Pharmacogenomics) Express Panel is offered in pre-spotted 384-well plates, an efficient, easy-to-use format for pharmacogenomics applications.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ QuantStudio™ Absolute Q™ Digital PCR Starter Kit
The QuantStudio Absolute Q Digital PCR Starter Kit is designed to confirm the performance of the QuantStudio Absolute Q Digital PCR System either at the time of system installation or as part of future, user-defined system verification procedures.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ GeneAmp™ 10X PCR Buffer
Applied Biosystems GeneAmp 10X PCR Buffer I is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Chemical Name or Material | PCR Buffer |
|---|---|
| Concentration | 10X |
| For Use With (Application) | Standard PCR |
Thermo Scientific™ DNA Polymerase I (10 U/μL)
DNA Polymerase I is a template-dependent DNA polymerase which catalyzes 5'→3' synthesis of DNA.
| Polymerase | DNA Polymerase I |
|---|---|
| Description | DNA Polymerase I |
| Concentration | 10 U/μL |
Thermo Scientific™ Taq DNA Polymerase, recombinant (1 U/μL)
Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus.
Invitrogen™ Second Strand cDNA Synthesis Kit
Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Reaction Format | Separate Components |
|---|---|
| Final Product Type | Double Stranded cDNA |
| Product Type | cDNA Synthesis Kit |
| For Use With (Application) | Cloning |
Applied Biosystems™ VetMAX™ Fast Multiplex Mastermix, with ROX
VetMAX Fast Multiplex Master Mix with ROX is designed to be a fast-cycling-capable, multiplex-assay-compatible one-step RT-PCR master mix for animal molecular diagnostics.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Polymerase | AmpliTaq™ Fast DNA Polymerase |
|---|---|
| Activation Time | 5 min. |
| Final Product Type | Mastermix |
| Form | Liquid |
| Reaction Speed | Fast |
| Label or Dye | ROX |
| Hot Start | Built-in Hot Start |
| Concentration | 2X |
| Starting Material | DNA/RNA |
| Compatibility | TaqMan™ |
| For Use With (Application) | Animal Health |
| PCR Method | 1-step RT-qPCR |
| Detection Method | Primer-probe |
Thermo Scientific™ Taq DNA Polymerase, native (5 U/μL)
Thermo Scientific Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus.
| Polymerase | Taq DNA Polymerase |
|---|---|
| Product Type | DNA Polymerase |
| Concentration | 5 U/μL |
| For Use With (Application) | Standard PCR |