PCR Supplies
Filtered Search Results
Applied Biosystems™ GeneAmp™ 10X PCR Buffer
Applied Biosystems GeneAmp 10X PCR Buffer I is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Chemical Name or Material | PCR Buffer |
|---|---|
| Concentration | 10X |
| For Use With (Application) | Standard PCR |
Applied Biosystems™ TaqPath™ BactoPure™ Microbial Detection Master Mix
TaqPath BactoPure Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Content And Storage | Contains 2X TaqPath BactoPure Microbial Detection Master Mix that includes: • Hot-start AmpliTaq Fast DNA Polymerase • Heat-labile uracil-DNA glycosylase (UNG) • dNTPs (with dUTP) • ROX dye (passive reference) • Optimized buffer Store at 2°C to 8°C in the dark. |
|---|---|
| For Use With (Application) | Microbial Detection |
| PCR Method | qPCR |
| Multiplex Capability | Up to 3-plex |
| Detection Method | Primer-probe |
| Includes | dUTP, UNG/UDG |
| Thermostability | 72 hr., Assembled Reaction |
| Sensitivity | Down to 1 copy |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio12k Flex Dx, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiA™ 7 System, ViiA 7 Dx, QuantStudio™ Dx, StepOnePlus™ System, 7500 Fast Dx System, StepOne™ System |
| Sample Type | DNA |
| Reaction Speed | Fast or Standard |
| Inhibitor Tolerance (Simple) | Very High |
| Target Specificity | cDNA or gDNA only |
| Product Line | TaqPath |
Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More
The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 2X |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| High-throughput Compatibility | High-throughput Compatible |
| PCR Method | 2-step RT-qPCR |
| Detection Method | SYBR |
| Format | Tube |
| Product Type | Fast Advanced Cells-to-Ct Kit |
| Sample Type | Mammalian Cells |
| Reaction Speed | 7 min. |
| Reagent Type | Cell Lysis |
| Purification Target | Lysate |
| Starting Material | 10-100,000 Cells |
| Product Line | Cells-to-CT |
Invitrogen™ Second Strand cDNA Synthesis Kit
Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Reaction Format | Separate Components |
|---|---|
| Final Product Type | Double Stranded cDNA |
| Product Type | cDNA Synthesis Kit |
| For Use With (Application) | Cloning |
Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix, no ROX™
Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | Medium |
| Content And Storage | Store at –15 to –25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use. |
| Form | Liquid |
| For Use With (Application) | Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence |
| PCR Method | 1-step RT-qPCR |
| Multiplex Capability | Up to 6-plex |
| Detection Method | Primer-probe |
| Includes | dUTP |
| Thermostability | 8 hr., Assembled Reaction |
| Polymerase | Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start |
| Format | Single-Tube |
| Passive Reference Dye | None |
| Reaction Speed | Fast or Standard |
| Inhibitor Tolerance (Simple) | High |
| Target Specificity | RNA only, Both RNA and DNA |
| Hot Start | Antibody-based |
| Shelf Life | Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt |
| Product Line | TaqPath |
Applied Biosystems™ VetMAX™ Fast Multiplex Mastermix, with ROX
VetMAX Fast Multiplex Master Mix with ROX is designed to be a fast-cycling-capable, multiplex-assay-compatible one-step RT-PCR master mix for animal molecular diagnostics.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Polymerase | AmpliTaq™ Fast DNA Polymerase |
|---|---|
| Activation Time | 5 min. |
| Final Product Type | Mastermix |
| Form | Liquid |
| Reaction Speed | Fast |
| Label or Dye | ROX |
| Hot Start | Built-in Hot Start |
| Concentration | 2X |
| Starting Material | DNA/RNA |
| Compatibility | TaqMan™ |
| For Use With (Application) | Animal Health |
| PCR Method | 1-step RT-qPCR |
| Detection Method | Primer-probe |
Applied Biosystems™ AmpliTaq™ DNA Polymerase with Buffer I or Buffer II
AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Shipping Condition | Dry Ice |
|---|---|
| Fidelity (vs. Taq) | 1X |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| For Use With (Application) | Standard PCR |
| Exonuclease Activity | 5' - 3' |
| Polymerase | AmpliTaq DNA Polymerase |
| Format | Stand-alone enzyme |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Reaction Speed | Standard |
| Hot Start | No |
| Starting Material | DNA |
Thermo Scientific™ Phusion™ HF and GC Buffer Packs
5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.
| Quantity | 4 x 1.5 mL |
|---|---|
| For Use With (Application) | Standard PCR |
Thermo Scientific™ T4 DNA Polymerase (5 U/μL)
Ensure accuracy in DNA synthesis with template-dependent T4 DNA Polymerase that catalyzes the 5'-3' synthesis from primed single-stranded DNA.
| Polymerase | T4 DNA Polymerase |
|---|---|
| Concentration | 5 U/μL |
Thermo Scientific™ RevertAid RT Reverse Transcription Kit
The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates.
Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix
TaqPath 1-Step Multiplex Master Mix is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Shipping Condition | Dry Ice |
|---|---|
| Concentration | 4X |
| For Use With (Application) | Gene Expression,Microbial Detection |
| PCR Method | 1-step RT-qPCR |
| Multiplex Capability | Up to 4-plex |
| Detection Method | Primer-probe |
| Includes | dUTP, UNG/UDG |
| Thermostability | Not Applicable |
| Polymerase | Fast DNA Polymerase |
| Sensitivity | Down to 10 copies |
| Format | Tube |
| Product Type | qPCR Master Mix |
| Passive Reference Dye | Mustang Purple |
| For Use With (Equipment) | QuantStudio 6 Pro Real-Time PCR System or QuantStudio 7 Pro, QuantStudio 3 or 5, QuantStudio 6/QuantStudio 7 Flex, QuantStudio 12K Flex, QuantStudio Dx, ViiA 7, 7500 , 7500 Fast, 7500 Fast Dx |
| Sample Type | DNA, RNA |
| Reaction Speed | Fast or Standard |
| Inhibitor Tolerance (Simple) | High |
| Target Specificity | RNA only, Both RNA and DNA |
| Product Line | TaqPath |
Thermo Scientific™ dNTP Mix (2 mM each)
Thermo Scientific dNTP Mix (2 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 2 mM.
| Shipping Condition | Dry Ice |
|---|---|
| Purity or Quality Grade | >99% |
| Form | Liquid |
| Label or Dye | Unlabeled |
| Concentration | 2 mM |
| For Use With (Application) | Standard PCR,High-fidelity PCR,Real Time PCR (qPCR) |
Applied Biosystems™ TrueMark™ Respiratory Panel 2.0 Amplification Control
The TrueMark Respiratory Panel 2.0 Amplification Control contains a linearized multi-target plasmid with target sequences for each respiratory tract microbiota assay on the TrueMark Respiratory Panel 2.0, OpenArray plate and TrueMark Respiratory Panel 2.0, TaqMan array card.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Invitrogen™ PureLink™ PCR Micro Kit
PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Shipping Condition | Room Temperature |
|---|---|
| Separation Range | 100 bp to 12 kb |
| Content And Storage | Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature. |
| Starting Material Volume | 125 μL |
| Isolation Technology | Silica Spin Column |
| Elution Volume Max | 20 μL |
| For Use With (Application) | Cloning,Standard PCR |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| Target | DNA from Enzymatic Reactions (e.g. PCR) |
| Format | Spin Column |
| Product Type | PCR Micro Kit |
| Run Time | 10 min. |
| Elution Volume Min | 5 μL |
| Product Line | PureLink |
Applied Biosystems™ TaqPath™ qPCR Master Mix, CG
TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Content And Storage | Store at 2°C to 8°C in the dark. |
|---|---|
| Form | Liquid |
| Concentration | 2X |
| For Use With (Application) | Gene Expression,Microbial Detection,DNA Quantification,Presence/Absence,miRNA analysis |
| PCR Method | qPCR |
| Multiplex Capability | Duplex |
| Detection Method | Primer-probe |
| Includes | dUTP, UNG/UDG |
| Thermostability | 48 hr., Assembled Reaction |
| Polymerase | Fast DNA Polymerase |
| Sensitivity | Down to 10 copies |
| Format | Bottle |
| Product Type | qPCR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | QuantStudio Dx, 7500 Fast Dx |
| Sample Type | cDNA, DNA |
| Reaction Speed | Fast or Standard |
| Inhibitor Tolerance (Simple) | High |
| Target Specificity | cDNA or gDNA only |
| Hot Start | Built-In Hot Start |
| Product Line | TaqPath |