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PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.
Real Time PCR Products (1,814)
Standard PCR Products (1,143)
DNA Polymerases (1,029)
Reverse Transcription Products (266)
dNTPs (232)

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Keyword Search:
76 – 90 of 3,795 results
76

Thermo Scientific™ dNTP Mix (2 mM each)
GSA_VA

Thermo Scientific dNTP Mix (2 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 2 mM.

EDGE
Shipping Condition Dry Ice
Purity or Quality Grade >99%
Form Liquid
Label or Dye Unlabeled
Concentration 2 mM
For Use With (Application) Standard PCR,High-fidelity PCR,Real Time PCR (qPCR)
77

Thermo Scientific™ Phire Hot Start II DNA Polymerase

Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.

EDGE
Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 2X
Reaction Format Separate Components
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Starting Material DNA
For Use With (Application) Cloning,Hot-start PCR
78

Thermo Scientific™ Phusion™ Hot Start II DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 52X
Size (Final Product) 20 kb or less
Reaction Format Standalone
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2 U/μL
For Use With (Application) Hot-start PCR,High-fidelity PCR
79

Invitrogen™ Poly(A) Tailing Kit

For the polyadenylation of in vitro transcribed RNA to enhance translation initiation efficiency.

EDGE ENCOMPASS
80
Promotions Available

Thermo Scientific™ Phusion U Multiplex PCR Master Mixes

Thermo Scientific Phusion U Multiplex PCR Master Mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

EDGE
Shipping Condition Dry Ice
Polymerase Phusion U Hot Start DNA Polymerase
Fidelity (vs. Taq) 25X
Size (Final Product) 2.5 kb or less
Reaction Format SuperMix or Master Mix
Product Type Multiplex PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
81

Applied Biosystems™ TrueMark™ Respiratory Panel 2.0 Amplification Control

The TrueMark Respiratory Panel 2.0 Amplification Control contains a linearized multi-target plasmid with target sequences for each respiratory tract microbiota assay on the TrueMark Respiratory Panel 2.0, OpenArray plate and TrueMark Respiratory Panel 2.0, TaqMan array card.

EDGE ENCOMPASS
82

Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/μL)
GSA_VA

Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain.

EDGE
Shipping Condition Dry Ice
Content And Storage

• RevertAid H Minus Reverse Transcriptase (200 U/μL)
• 5X Reaction Buffer

Store at –20°C.
Reverse Transcriptase RevertAid H Minus
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 13 kb
Reaction Format Separate components
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 42°C to 45°C
Technique Reverse Transcription
Starting Material RNA
83

Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix

Thermo Scientific Maxima H Minus cDNA Synthesis Master Mix, available with or without dsDNase, provides all cDNA synthesis reaction components in a convenient one-tube master mix.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase Maxima H Minus
Final Product Type First-Strand cDNA
Quantity Each
For Use With (Application) Real Time PCR (qPCR),RT-PCR
Format Master Mix
Size (Final Product) Up to 20 kb
Reaction Format Premixed Components
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
84
Promotions Available

Applied Biosystems™ TaqPath™ qPCR Master Mix, CG

TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.

EDGE ENCOMPASS
Content And Storage Store at 2°C to 8°C in the dark.
Form Liquid
InhibitorToleranceSimple High
Concentration 2X
For Use With (Application) Gene Expression,Microbial Detection,DNA Quantification,Presence/Absence,miRNA analysis
PCR Method qPCR
Multiplex Capability Duplex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 48 hr., Assembled Reaction
Polymerase Fast DNA Polymerase
Sensitivity Down to 10 copies
Format Bottle
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) QuantStudio Dx, 7500 Fast Dx
Sample Type cDNA, DNA
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Hot Start Built-In Hot Start
Product Line TaqPath
85

Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0

Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.

EDGE ENCOMPASS
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,Pathogen Detection,Virus Detection
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Bottle
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
Sample Type Environmental Sample, Food
Reaction Speed Standard
Hot Start Built-In Hot Start
Product Line TaqMan
86
Promotions Available

Applied Biosystems™ TaqMan™ RNase P Detection Reagents Kit

TaqMan™ RNase P Detection Reagents Kit

EDGE ENCOMPASS
87
Promotions Available

Applied Biosystems™ TaqMan™ RNase P Control Reagents Kit

TaqMan™ RNase P Control Reagents Kit

EDGE ENCOMPASS
88

Invitrogen™ dCTP (100 mM)

Invitrogen dCTP is prepared to a final concentration of 100 mM in highly purified water. 100 mM dCTP is suitable for use in polymerase chain reaction (PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, and TdT-tailing reactions.

EDGE ENCOMPASS
89

Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase

Thermo Scientific DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.

EDGE
Polymerase DreamTaq DNA Polymerase
GC-Rich PCR Performance Low
Reaction Format Separate Components
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 5 U/μL
For Use With (Application) Hot-start PCR
90

Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR

TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.

EDGE ENCOMPASS
Shipping Condition Dry Ice
Reverse Transcriptase M-MLV
Form Liquid
InhibitorToleranceSimple High
For Use With (Application) Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence
PCR Method 1-step RT-qPCR
Multiplex Capability Up to 3-plex
Detection Method Primer-probe
Thermostability Not Applicable
Polymerase AmpliTaq Fast DNA Polymerase
Format Tube
Product Type 1-Step Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System
Sample Type Total RNA, Viral DNA, Viral RNA, mRNA
Reaction Speed Fast or Standard
Target Specificity RNA only, Both RNA and DNA
Hot Start Built-In Hot Start
Product Line TaqMan