PCR Supplies
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Thermo Scientific™ Phusion Hot Start II High-Fidelity PCR Master Mixes
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion Hot Start II High-Fidelity DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Hot Start High-Fidelity PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Applied Biosystems™ AmpErase™ Uracil N-Glycosylase (UNG)
Enzyme removes any uracil incorporated into single- or double-stranded DNA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ dNTP Mix (2.5 mM)
Invitrogen 2.5 mM dNTP (2'-deoxynucleoside 5'-triphosphate) Mix consists of a solution of all four nucleotides, dATP, dCTP, dGTP, and dTTP, each at a concentration of 2.5 mM.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Fidelity (vs. Taq) | 25 X |
| Description | Works on all AB platforms, but is optimized for the 7500. |
| Reverse Transcriptase | ArrayScript™ |
| Concentration | 25X |
| PCR Method | 1-step RT-qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Format | Tube |
| Product Type | One-Step RT-PCR Reagent |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 System |
| Reaction Speed | Standard |
| Product Line | AgPath-ID, Ambion |
Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 2X |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| High-throughput Compatibility | High-throughput Compatible |
| PCR Method | 2-step RT-qPCR |
| Detection Method | SYBR |
| Format | Tube |
| Product Type | Fast Advanced Cells-to-Ct Kit |
| Sample Type | Mammalian Cells |
| Reaction Speed | 7 min. |
| Reagent Type | Cell Lysis |
| Purification Target | Lysate |
| Starting Material | 10-100,000 Cells |
| Product Line | Cells-to-CT |
Thermo Scientific Chemicals SSC buffer (20X)
Suitable for preparing nucleic acids for hybridization, hybridization buffer for northern and southern blots, washing buffer for northern and southern blots. Saline-Sodium Citrate Buffer (SSC) is used as a hybridization buffer in immunoblotting and DNA Microarray protocols.
| Physical Form | Liquid |
|---|---|
| Recommended Storage | Ambient temperatures |
Thermo Scientific™ SureTect™ Campylobacter jejuni, C. coli and C. lari PCR Assay
Rapidly detect Campylobacter jejuni, C. coli and C. lari in food and environmental samples.
Applied Biosystems™ TaqMan™ MGB Probe
Custom TaqMan probes are dual-labeled primers suitable for use in any real-time PCR application. They incorporate a 5' reporter dye and a 3' nonfluorescent quencher/minor groove binder (MGB). Choose among FAM, VIC, ABY, JUN, Cy5, TET, and NED as reporters.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Purification Method | HPLC |
|---|---|
| Purity or Quality Grade | HPLC |
| Format | Tube |
| Internal Probe Modification | None |
| For Use With (Equipment) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System |
| Form | Liquid |
| 3'Primer Modification | MGB-NFQ |
| For Use With (Application) | Real Time PCR (qPCR) |
| Product Line | TaqMan |
Applied Biosystems™ TaqPath™ qPCR Master Mix, CG
TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Content And Storage | Store at 2°C to 8°C in the dark. |
|---|---|
| Form | Liquid |
| Concentration | 2X |
| For Use With (Application) | Gene Expression,Microbial Detection,DNA Quantification,Presence/Absence,miRNA analysis |
| PCR Method | qPCR |
| Multiplex Capability | Duplex |
| Detection Method | Primer-probe |
| Includes | dUTP, UNG/UDG |
| Thermostability | 48 hr., Assembled Reaction |
| Polymerase | Fast DNA Polymerase |
| Sensitivity | Down to 10 copies |
| Format | Bottle |
| Product Type | qPCR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | QuantStudio Dx, 7500 Fast Dx, Roche LightCycler PRO System, Bio-Rad CFX Opus 96 Real-Time PCR System |
| Sample Type | cDNA, DNA |
| Reaction Speed | Fast or Standard |
| Inhibitor Tolerance (Simple) | High |
| Target Specificity | cDNA or gDNA only |
| Hot Start | Built-In Hot Start |
| Product Line | TaqPath |
Invitrogen™ Second Strand cDNA Synthesis Kit
Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Reaction Format | Separate Components |
|---|---|
| Final Product Type | Double Stranded cDNA |
| Product Type | cDNA Synthesis Kit |
| For Use With (Application) | Cloning |
Invitrogen™ GeneArt™ Genomic Cleavage Detection Kit
The GeneArt Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method for the detection of locus-specific double-strand break formation.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain.
| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | • RevertAid H Minus Reverse Transcriptase (200 U/μL) |
| Reverse Transcriptase | RevertAid H Minus |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 13 kb |
| Reaction Format | Separate components |
| Reaction Speed | 60 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 42°C to 45°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Applied Biosystems™ GeneAmp™ 10X PCR Buffer II & MgCl2
Applied Biosystems GeneAmp 10X PCR Buffer II is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Polymerase | AmpliTaq DNA Polymerase |
|---|---|
| Fidelity (vs. Taq) | 1X |
| Reaction Format | Kit |
| Product Type | PCR Buffer |
| Concentration | 10X |
| For Use With (Application) | Standard PCR |
Invitrogen™ Buffer Kit, RNase-free
The Invitrogen Buffer Kit provides high quality, RNase free buffers for critical RNA analysis procedures.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ TaqPath™ BactoPure™ Microbial Detection Master Mix
TaqPath BactoPure Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Content And Storage | Contains 2X TaqPath BactoPure Microbial Detection Master Mix that includes: • Hot-start AmpliTaq Fast DNA Polymerase • Heat-labile uracil-DNA glycosylase (UNG) • dNTPs (with dUTP) • ROX dye (passive reference) • Optimized buffer Store at 2°C to 8°C in the dark. |
|---|---|
| For Use With (Application) | Microbial Detection |
| PCR Method | qPCR |
| Multiplex Capability | Up to 3-plex |
| Detection Method | Primer-probe |
| Includes | dUTP, UNG/UDG |
| Thermostability | 72 hr., Assembled Reaction |
| Sensitivity | Down to 1 copy |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio12k Flex Dx, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiA™ 7 System, ViiA 7 Dx, QuantStudio™ Dx, StepOnePlus™ System, 7500 Fast Dx System, StepOne™ System |
| Sample Type | DNA |
| Reaction Speed | Fast or Standard |
| Inhibitor Tolerance (Simple) | Very High |
| Target Specificity | cDNA or gDNA only |
| Product Line | TaqPath |