KAPA HiFi HotStart ReadyMix is a 2X PCR master mix optimized for high-fidelity PCR amplification of DNA templates up to 5 kb. It contains KAPA HiFi HotStart DNA Polymerase, dNTPs, MgCl2 and stabilizers. The hot start polymerase allows room temperature reaction setup without non-specific amplification. KAPA HiFi HotStart ReadyMix delivers high yields and minimal bias for a wide range of targets, and is suitable for PCR applications including next-generation sequencing library construction, site-directed mutagenesis, and cloning of GC-rich targets.