Real Time PCR Assays and Arrays
AMID BIOSCIENCES Amid Biosciences, CypA - KRAS G12V Protein Kit, Molecular Glue Discovery, 1 Kit
This CypA and KRAS G12V protein kit supports in vitro studies of Cyclophilin A driven complex formation with oncogenic KRAS G12V. Applications include molecular glue discovery, CypA-KRAS G12V ternary complex research, binding assay development, SPR, BLI, AlphaScreen, TR-FRET, pull-down workflows. Technical specifications are included below.- Quantity - Kit- Organism - Human- Purity - greater than 90%- Storage - -80 C - Biotinylated - Protein component dependent- Components - Recombinant Biotinylated Human KRAS G12V (1-185), 0.05 mg, featuring a specific G12V oncogenic mutation on the switch-sense domain of the isoform KRAS4B. Recombinant Biotinylated Human Wild-Type KRAS (1-185), 0.05 mg, included for critical counter-screening and selectivity profiling. Recombinant Human Cyclophilin A (CypA) (1-165), 0.1 mg, tagged with an N-terminal 6XHis tag for versatile assay orientation and capture. This product is for research use only.
AURORA BIOLABS LLC Kras G12V Activity Assay, 384 rxn
The Aurora Biolabs KRAS G12V Nucleotide Exchange Assay Kit is a TR-FRET based assay designed to detect GTP loading on KRAS G12V in the presence of SOS1, a guanine nucleotide exchange factor. This kit uses Tag2-labeled KRAS protein and a terbium-labeled anti-Tag2 antibody (HTRF donor). When KRAS binds to fluorescently labeled GTP (HTRF acceptor), the donor and acceptor come into close proximity. Upon excitation at 340nm, energy transfer occurs, and emission at 665nm is measured. This assay quantitatively detects active GTP-bound KRAS G12V, ideal for inhibitor screening. Each kit supports 384 reactions.
AMID BIOSCIENCES Amid Biosciences, Human KRAS G12D Protein, Recombinant, Biotinylated, 0.1 mg
Human KRAS G12D is supplied as a recombinant biotinylated protein in a 0.1 mg format for oncology drug discovery and RAS pathway research. Applications include SPR, BLI, ELISA, Pull-down, AlphaScreen, MST, protein-protein interaction studies, drug discovery workflows. Technical specifications are included below.- Quantity - 0.1 mg- Gene - KRAS- Mutation / Variant - G12D- Organism - Human- Tag - N-terminal AviTag, N-terminal 8XHis- Expression System - E. coli- Purity - greater than 90% by Coomassie staining- UniProt ID - P01116-2- Amino Acid Range - 1-185- Buffer - 20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercapthoethanol, 10% glycerol- Endotoxin Level - less than 1 EU/mg- Storage - -80 C- Biotinylated - YesThis product is for research use only.
ABclonal Technology AFTSpin Tissue/Cell Fast RNA Extraction Kit for Animal
This kit does not rely on toxic reagents such as phenol and chloroform, and can be used for rapid RNA extraction from common animal tissue and cell samples. The excellent silica gel column purification technology can effectively remove residual gDNA and quickly extract animal total RNA. Extracted RNA can be directly used in reverse transcription PCR, qPCR and RNA library construction experiments.
Sino Biological Recombinant Cynomolgus, Rhesus Transferrin Receptor/TFR1/CD71 Protein (His Tag), Endotoxin-Free
A DNA sequence encoding the cynomolgus/rhesus TFRC (XP_005545315.1 & NP_001244232.1) (Cys89-Phe760) was expressed with a polyhistidine tag at the N-terminus. Cynomolgus and Rhesus TFRC sequences are identical.
Sino Biological Recombinant Human Transferrin Receptor/TFR1/CD71 Protein (ECD, His Tag), HPLC-verified, Endotoxin-Free
A DNA sequence encoding the extracellular domain of human TFRC (AAA61153.1) (Cys89-Phe760) was expressed with a polyhistidine tag at the N-terminus.
Genscript Corporation KRAS His Human G12D
The KRAS gene provides instructions for making a protein called K-Ras part of the RAS/MAPK pathway The protein relays signals from outside the cell to the cell s nucleus These signals instruct the cell to grow and divide (proliferate) or to mature and take on specialized functions (differentiate) The K-Ras protein is a GTPase which means it converts a molecule called GTP into another molecule called GDP In this way the K-Ras protein acts like a switch that is turned on and off by the GTP and GDP molecules KRAS is usually tethered to cell membranes because of the presence of an isoprene group on its C-terminus There are two protein products of the KRAS gene in mammalian cells that result from the use of alternative exon 4 (exon 4A and 4B respectively) K-Ras4A and K-Ras4B these proteins have different structure in their C-terminal region and use different mechanisms to localize to cellular membranes including the plasma membrane