Real Time PCR Master Mixes

Real Time PCR Master Mixes

Real-time PCR master mixes are batch mixtures of PCR reagents at optimal concentrations that can be prepared and divided among multiple PCR tubes/plates. They reduce pipetting and risk of contamination, save time, and limit possible mixing errors.
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1 – 30 of 139 results
1

Thermo Scientific™ ABsolute Blue QPCR Mix, ROX

ABsolute Blue qPCR Mixes utilize Thermo-Start Taq hot start enzyme for increased sensitivity.

Content And Storage Contains:
• 1 x 5 mL ABsolute Blue qPCR ROX Mix (2X)
Sufficient for 400 x 25 µL reactions.

Store at -20°C
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type DNA, dsDNA
Detection Method Primer-Probe Detection
2

Thermo Scientific™ ABsolute Blue QPCR Mix, SYBR Green, with separate ROX vial

Incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

Sample Type DNA, dsDNA
For Use With (Application) Real Time PCR (qPCR)
Detection Method SYBR
3

Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, low ROX

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Content And Storage Contains:
• 4 x 12.5 mL Luminaris HiGreen Low ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 5000 x 20 µL reactions. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms.
• 2 x 30 mL Nuclease-Free Water

Store at -20°C.
For Use With (Equipment) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type DNA, dsDNA
For Use With (Application) qPCR, Two-Step RT-qPCR
Detection Method SYBR
4

Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix, with dsDNase

Provide one-tube master mix for convenient and consistent cDNA synthesis for two-step RT-qPCR applications. The format helps streamline the RT-qPCR workflow, saves time, reduces pipeting errors to give customers sensitive, reliable and fast cDNA synthesis.

For Use With (Application) Real-Time PCR
5

Thermo Scientific™ ABsolute QPCR Mix, SYBR Green, ROX

Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.

Sample Type DNA, dsDNA
For Use With (Application) Real Time PCR (qPCR)
Detection Method SYBR
6

Thermo Scientific™ ABsolute QPCR Mix, SYBR Green, fluorescein

Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Sample Type DNA, dsDNA
Compatibility qPCR Cyclers
Detection Method SYBR
7

Thermo Scientific™ ABsolute QPCR Mix, low ROX

Optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.

Content And Storage Contains:
• 1 x 5 mL ABsolute QPCR ROX Low Mix (2X)

Store at -20°C
For Use With (Equipment) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type DNA, dsDNA
For Use With (Application) Real Time PCR (qPCR)
Detection Method Primer-Probe Detection
8

Thermo Scientific™ ABsolute QPCR Mix, SYBR Green, low ROX

Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.

Sample Type DNA, dsDNA
For Use With (Application) Real Time PCR (qPCR)
Detection Method SYBR
9

Thermo Scientific™ Maxima SYBR Green/ROX qPCR Master Mix (2X)
GSA_VA

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

Sample Type DNA, dsDNA
For Use With (Application) Real Time PCR (qPCR)
Detection Method SYBR
10

Thermo Scientific™ Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
GSA_VA

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

Sample Type DNA, dsDNA
For Use With (Application) Real Time PCR (qPCR)
Detection Method SYBR
11

Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix, high ROX

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Content And Storage Contains:
• 4 x 12.5 mL Luminaris Color HiGreen High ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer with a blue dye; sufficient for 5000 x 20 µL reactions. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms.
• 1 x 13 mL 40X Yellow Sample Buffer
• 2 x 30 mL Nuclease-Free Water

Store at -20°C.
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type DNA, dsDNA
For Use With (Application) qPCR, Two-Step RT-qPCR
Detection Method SYBR
12

Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix, low ROX

Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Content And Storage Contains:
• 4 x 12.5 mL Luminaris Color HiGreen Low ROX qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer with a blue dye; sufficient for 5000 x 20 µL reactions. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms.
• 1 x 13 mL 40X Yellow Sample Buffer
• 2 x 30 mL Nuclease-Free Water

Store at -20°C.
For Use With (Equipment) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type DNA, dsDNA
For Use With (Application) qPCR, Two-Step RT-qPCR
Detection Method SYBR
13

Thermo Scientific™ DyNAmo HS SYBR Green qPCR Kit

Contains a modified Thermus brockianus DNA polymerase and all the other reagents needed for qPCR.

Sample Type DNA, dsDNA
For Use With (Application) Real Time PCR (qPCR)
Detection Method SYBR
14

Thermo Scientific™ DyNAmo ColorFlash SYBR Green qPCR Kit

Offers equal performance to the DyNAmo Flash SYBR Green qPCR Kit. In addition, it incorporates an innovative multi-color system that ensures correct pipetting.

Sample Type DNA, dsDNA
For Use With (Application) Real Time PCR (qPCR)
Detection Method SYBR
15

Thermo Scientific™ DyNAmo Flash SYBR Green qPCR Kit

Developed for fast real-time qPCR, the kit provides qPCR results faster than most SYBR Green kits without compromising the qPCR performance.

Sample Type DNA, dsDNA
For Use With (Application) Real Time PCR (qPCR)
Detection Method SYBR
16

Thermo Scientific™ DyNAmo Flash Probe qPCR Kit

Perfect choice for fast probe-based qPCR. The optimized reagents and extremely short cycling times deliver maximum speed thus increasing sample throughput.

Sample Type DNA, dsDNA
For Use With (Application) Real Time PCR (qPCR)
Detection Method Primer-Probe Detection