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Real Time PCR Master Mixes

Real-time PCR master mixes are batch mixtures of PCR reagents at optimal concentrations that can be prepared and divided among multiple PCR tubes/plates. They reduce pipetting and risk of contamination, save time, and limit possible mixing errors.
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1 – 15 of 373 results
1

Fisherbrand™ SYBR™ Green qPCR Master Mix

Fisherbrand™ SYBR™ Green qPCR Master Mix is a 2X, ready-to-use master mix designed for dye-based, quantitative amplification of DNA (cDNA and gDNA) targets by real-time PCR.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Content And Storage SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark
Product Type Master Mix
Form Frozen
Reaction Speed Fast or Standard
Label or Dye SYBR Green,FAM
Concentration 2X
For Use With (Application) Gene Expression,DNA Quantitation,Microbial Detection,Real Time PCR (qPCR)
PCR Method qPCR,2-step RT-qPCR,RT-qPCR
Detection Method SYBR
2

Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix (No ROX)

TaqPath 1-Step Multiplex Master Mix (No ROX) is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
InhibitorToleranceSimple High
For Use With (Application) Microbial Detection,DNA Quantitation,Presence/Absence
PCR Method 1-step RT-qPCR
Multiplex Capability Up to 4-plex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability Not Applicable
Sensitivity Down to 10 copies
Format Tube
Passive Reference Dye None
For Use With (Equipment) QuantStudio™ 6 Pro or QuantStudio™ 7 Pro, QuantStudio™ 3 or 5, QuantStudio™ 6/QuantStudio™ 7 Flex, QuantStudio™ 12K Flex, QuantStudio™ Dx, QuantStudio™ Absolute Q Digital PCR System, ViiA™ 7, 7500, 7500 Fast, 7500 Fast Dx, or use a compatible real-time PCR instrument from another supplier
Sample Type DNA, RNA
Reaction Speed Fast or Standard
Target Specificity RNA only, Both RNA and DNA
Product Line TaqPath™
3
Promotions Available

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Wet Ice
InhibitorToleranceSimple Medium
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Multiplex Capability Single-plex
Detection Method SYBR
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Polymerase Dual-Lock Taq DNA Polymerase
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, QuantStudio™ Absolute Q Digital PCR System, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type DNA
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Product Line PowerUP™, SYBR™
4

Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Content And Storage • PowerTrack SYBR Green Master Mix
• 40X Yellow Sample Buffer

Store at -20±5°C in the dark.
InhibitorToleranceSimple High
Concentration 2X
For Use With (Application) Gene Expression,Microbial Detection
PCR Method qPCR
Multiplex Capability Single-plex
Detection Method SYBR
Includes dUTP, UNG/UDG
Thermostability 8 hr., Assembled Reaction
Polymerase Taq DNA Polymerase
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, QuantStudio™ Absolute Q Digital PCR System, Bio-Rad CFX Connect™, Bio-Rad CFX384 Touch™, Bio-Rad CFX96 Touch™, BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA™ 7 System, StepOne™ System, MJ Chromo4, MJ Opticon, StepOnePlus™ System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Sample Type DNA
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Product Line Applied Biosystems™
5

Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix

TaqPath 1-Step Multiplex Master Mix is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
InhibitorToleranceSimple High
Concentration 4X
For Use With (Application) Gene Expression,Microbial Detection
PCR Method 1-step RT-qPCR
Multiplex Capability Up to 4-plex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability Not Applicable
Polymerase Fast DNA Polymerase
Sensitivity Down to 10 copies
Format Tube
Product Type qPCR Master Mix
Passive Reference Dye Mustang Purple
For Use With (Equipment) QuantStudio™ 6 Pro Real-Time PCR System or QuantStudio™ 7 Pro, QuantStudio™ 3 or 5, QuantStudio™ 6/QuantStudio™ 7 Flex, QuantStudio™ 12K Flex, QuantStudio™ Dx, QuantStudio™ Absolute Q Digital PCR System, ViiA™ 7, 7500 , 7500 Fast, 7500 Fast Dx
Sample Type DNA, RNA
Reaction Speed Fast or Standard
Target Specificity RNA only, Both RNA and DNA
Product Line TaqPath™
6
Promotions Available

Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix

Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
GC-Rich PCR Performance Medium
Content And Storage Store at -15°C to -25°C.
Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use.
Form Liquid
InhibitorToleranceSimple High
Concentration 4X
For Use With (Application) Microbial Detection,Gene Expression,DNA Quantitation,Presence/Absence
PCR Method 1-step RT-qPCR
Multiplex Capability Up to 5-plex
Detection Method Primer-probe
Includes dUTP
Thermostability 8 hr., Assembled Reaction
Polymerase Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start
Format Single-Tube
Passive Reference Dye ROX (Pre-mixed)
Reaction Speed Fast or Standard
Target Specificity RNA only, Both RNA and DNA
Hot Start Antibody-based
Shelf Life Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt
Product Line TaqPath™
7

Applied Biosystems™ TaqPath™ BactoPure™ Microbial Detection Master Mix

TaqPath BactoPure Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors.

EDGE ENCOMPASS_PREFERRED
Content And Storage Contains 2X TaqPath BactoPure Microbial Detection Master Mix that includes:
• Hot-start AmpliTaq Fast DNA Polymerase
• Heat-labile uracil-DNA glycosylase (UNG)
• dNTPs (with dUTP)
• ROX dye (passive reference)
• Optimized buffer

Store at 2°C to 8°C in the dark.
InhibitorToleranceSimple Very High
For Use With (Application) Microbial Detection
PCR Method qPCR
Multiplex Capability Up to 3-plex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Sensitivity Down to 1 copy
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio12k Flex Dx, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiA™ 7 System, ViiA 7 Dx, QuantStudio™ Dx, StepOnePlus™ System, 7500 Fast Dx System, StepOne™ System
Sample Type DNA
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Product Line TaqPath™
8

Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix, no ROX™

Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
GC-Rich PCR Performance Medium
Content And Storage Store at –15 to –25°C.
Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use.
Form Liquid
InhibitorToleranceSimple High
For Use With (Application) Microbial Detection,Gene Expression,DNA Quantitation,Presence/Absence
PCR Method 1-step RT-qPCR
Multiplex Capability Up to 6-plex
Detection Method Primer-probe
Includes dUTP
Thermostability 8 hr., Assembled Reaction
Polymerase Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start
Format Single-Tube
Passive Reference Dye None
Reaction Speed Fast or Standard
Target Specificity RNA only, Both RNA and DNA
Hot Start Antibody-based
Shelf Life Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt
Product Line TaqPath™
9

Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0

Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.

EDGE ENCOMPASS_PREFERRED
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,Pathogen Detection,Virus Detection
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Bottle
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
Sample Type Environmental Sample, Food
Reaction Speed Standard
Hot Start Built-In Hot Start
Product Line TaqMan™
10
Promotions Available

Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Form Liquid
InhibitorToleranceSimple High
Concentration 2X
For Use With (Application) Gene Expression,DNA Quantitation,Presence/Absence,miRNA analysis
PCR Method qPCR
Multiplex Capability Duplex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Polymerase AmpliTaq Fast DNA Polymerase
Product Type Fast Advanced Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio Absolute Q Digital PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 6 Pro, QuantStudio™ 7 Flex, QuantStudio™ 7 Pro, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast
Target Specificity cDNA or gDNA only
Product Line TaqMan™
11
Promotions Available

Applied Biosystems™ TaqMan™ Universal Master Mix II, with UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

EDGE ENCOMPASS_PREFERRED
GC-Rich PCR Performance High
Content And Storage Store at 2°C to 8°C in the dark.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq) 2 X
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Product Type Universal Master Mix II
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Product Line TaqMan™
12
Promotions Available

Applied Biosystems™ Power SYBR™ Green PCR Master Mix

Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

EDGE ENCOMPASS_PREFERRED
GC-Rich PCR Performance High
Purity or Quality Grade LD (Low DNA)
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method SYBR
Polymerase AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Label or Dye SYBR Green
Product Line SYBR™
13

Applied Biosystems™ TaqPath™ 1-Step RT-qPCR Master Mix, CG

TaqPath 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX dye, and thermostable Fast DNA polymerase.

EDGE ENCOMPASS_PREFERRED
Reverse Transcriptase M-MLV
Form Liquid
InhibitorToleranceSimple High
Concentration 4X
For Use With (Application) Microbial Detection,DNA Quantitation,Presence/Absence
PCR Method 1-step RT-qPCR
Multiplex Capability Up to 3-plex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability Not Applicable
Polymerase Fast DNA Polymerase
Sensitivity Down to 10 copies
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System, 7500 Fast Dx System, QuantStudio™ Dx
Sample Type RNA, cDNA, DNA
Reaction Speed Fast or Standard
Target Specificity RNA only, Both RNA and DNA
Hot Start Built-In Hot Start
Product Line TaqPath™
14
Promotions Available

Applied Biosystems™ TaqPath™ qPCR Master Mix, CG

TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.

EDGE ENCOMPASS_PREFERRED
Content And Storage Store at 2°C to 8°C in the dark.
Form Liquid
InhibitorToleranceSimple High
Concentration 2X
For Use With (Application) Gene Expression,Microbial Detection,DNA Quantitation,Presence/Absence,miRNA analysis
PCR Method qPCR
Multiplex Capability Duplex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 48 hr., Assembled Reaction
Polymerase Fast DNA Polymerase
Sensitivity Down to 10 copies
Format Bottle
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) QuantStudio™ Dx, 7500 Fast Dx, QuantStudio™ Absolute Q Digital PCR System
Sample Type cDNA, DNA
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Hot Start Built-In Hot Start
Product Line TaqPath™
15

Applied Biosystems™ TaqPath™ BactoPure™ Microbial Detection Master Mix, no Rox

TaqPath™ BactoPure™ Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors. Does not contain a passive reference, recommended for use with assays utilizing JUN dye or higher order multiplexing (> three targets).

EDGE ENCOMPASS_PREFERRED
InhibitorToleranceSimple Very High
For Use With (Application) Microbial Detection
PCR Method qPCR
Multiplex Capability Up to 4-plex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Sensitivity Down to 1 copy
Passive Reference Dye None
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™ 12k Flex Dx, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiA™ 7 System, ViiA 7 Dx, QuantStudio™ Dx, StepOnePlus™ System, 7500 Fast Dx System, StepOne™ System
Sample Type DNA
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Product Line TaqPath™