Real Time PCR Master Mixes

Real Time PCR Master Mixes

Real-time PCR master mixes are batch mixtures of PCR reagents at optimal concentrations that can be prepared and divided among multiple PCR tubes/plates. They reduce pipetting and risk of contamination, save time, and limit possible mixing errors.
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PCR Method 
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GC-Rich PCR Performance

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1 – 30 of 140 results
1

Thermo Scientific™ ABsolute QPCR Mix, ROX

Optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.

PCR Method qPCR
Detection Method Primer-Probe
2

Thermo Scientific™ ABsolute QPCR Mix, SYBR Green, no ROX

Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Sample Type DNA, dsDNA
Compatibility qPCR Cyclers
Detection Method SYBR
3

Thermo Scientific™ ABsolute Blue QPCR Mix, SYBR Green, fluorescein

Incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

For Use With (Equipment) BioRad iCycler iQ, BioRad MyiQ, BioRad iQ5
Reaction Speed Standard
Sample Type DNA, dsDNA
For Use With (Application) Gene Expression,Genotyping,siRNA Validation
Detection Method SYBR
4

Thermo Scientific™ ABsolute Blue QPCR Mix, SYBR Green, low ROX

Incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

For Use With (Equipment) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Reaction Speed Standard
Sample Type DNA, dsDNA
For Use With (Application) Gene Expression,Genotyping,siRNA Validation
Detection Method SYBR
5

Thermo Scientific™ ABsolute Blue QPCR Mix, with separate ROX vial

ABsolute Blue qPCR Mixes utilize Thermo-Start Taq hot start enzyme for increased sensitivity.

PCR Method qPCR
Detection Method Primer-Probe
6

Thermo Scientific™ ABsolute Blue QPCR Mix, SYBR Green, ROX

Incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Reaction Speed Standard
Sample Type DNA, dsDNA
For Use With (Application) Gene Expression,Genotyping,siRNA Validation
Detection Method SYBR
7

Thermo Scientific™ ABsolute Blue QPCR Mix, low ROX

ABsolute Blue qPCR Mixes are 2X master mixes for probe chemistry. There are suitable alternatives available for all common real-time instruments.

PCR Method qPCR
Detection Method Primer-Probe
8

Thermo Scientific™ Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial
GSA_VA

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Reaction Speed Standard
Sample Type DNA, dsDNA
For Use With (Application) Gene Expression
Detection Method SYBR
9

Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Content And Storage Contains:
• 4 x 12.5 mL Luminaris Color HiGreen qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer with a blue dye; sufficient for 5000 x 20 µL reactions
• 1 x 13 mL 40X Yellow Sample Buffer
• 2 x 30 mL Nuclease-Free Water

Store at -20°C.
Fidelity (vs. Taq) 2 X
Product Type qPCR Master Mix
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Reaction Speed Standard
Sample Type DNA, dsDNA
Concentration 2X
PCR Method qPCR
For Use With (Application) Gene Expression
Product Line Luminaris
Detection Method SYBR
10

Thermo Scientific™ Luminaris HiGreen qPCR Master Mix

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type qPCR Master Mix
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Reaction Speed Standard
Sample Type DNA, dsDNA
Concentration 2X
PCR Method qPCR
For Use With (Application) Gene Expression
Product Line Luminaris
Detection Method SYBR
11

Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, high ROX

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type qPCR Master Mix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Reaction Speed Standard
Sample Type DNA, dsDNA
Concentration 2X
PCR Method qPCR
For Use With (Application) Gene Expression
Product Line Luminaris
Detection Method SYBR
12

Thermo Scientific™ DyNamo ColorFlash Probe qPCR Kit

Offers equal performance to the DyNAmo Flash Probe qPCR Kit. In addition, it incorporates an innovative multicolor system that ensures correct pipetting.

Reaction Speed Fast
Concentration 2X (DyNAmo ColorFlash qPCR Mix), 50X (ROX Dye), 40X (Sample Buffer Solution with Yellow Dye)
PCR Method qPCR
Product Line DyNAmo Flash
13

Thermo Scientific™ DyNAmo HS SYBR Green qPCR Kit

Contains a modified Thermus brockianus DNA polymerase and all the other reagents needed for qPCR.

For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, StepOne™ System, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, Cepheid SmartCycler
Sample Type DNA, dsDNA
Reaction Speed Standard
For Use With (Application) Gene Expression
Detection Method SYBR
14

Thermo Scientific™ DyNAmo Flash Probe qPCR Kit

Perfect choice for fast probe-based qPCR. The optimized reagents and extremely short cycling times deliver maximum speed thus increasing sample throughput.

Sample Type DNA, dsDNA
Concentration 2X (DyNAmo Flash qPCR Mix), 50X (ROX Dye)
PCR Method qPCR
For Use With (Application) Gene Expression
15

Thermo Scientific™ DyNAmo ColorFlash SYBR Green qPCR Kit

Offers equal performance to the DyNAmo Flash SYBR Green qPCR Kit. In addition, it incorporates an innovative multi-color system that ensures correct pipetting.

For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, StepOne™ System, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, Cepheid SmartCycler
Sample Type DNA, dsDNA
Reaction Speed Fast
For Use With (Application) Gene Expression
Detection Method SYBR
16

Thermo Scientific™ DyNAmo Flash SYBR Green qPCR Kit

Developed for fast real-time qPCR, the kit provides qPCR results faster than most SYBR Green kits without compromising the qPCR performance.