Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
New England Biolabs, Inc. Luna Universal Probe One-Step RT-qPCR Kit – 2500 reactions
In the Luna Universal One-Step Probe RT-qPCR Kit, Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling, providing added security for setting up reactions at room temperature. The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. Note that to ensure full activation of the WarmStart Luna RT, incubation at temperatures lower than 50°C is not recommended.
Zymo Research Corporation Quick-cfRNA Serum & Plasma Kit
The Quick-cfRNA Serum & Plasma Kit enables simple, reliable, and efficient preparation of high-quality circulating cell-free RNA (including protein-bound, exosomal, miRNA and other small RNAs) from serum, plasma and other biological fluids. The kit does not use toxic phenol-chloroform or cumbersome protein precipitation and removal steps. Recovered cell-free RNA scales linearly relative to the sample input volume up to 3 ml. ZymoSpin™ Technology enables isolation of ultra-pure cell-free RNA suitable for all subsequent analyses, such as RT-qPCR and NGS. Empower your discovery of circulating RNA biomarkers.
New England Biolabs, Inc. Luna Probe One-Step RT-qPCR Kit (No ROX) – 2500 reactions
In the Luna Probe One-Step RT-qPCR Kit (No ROX), Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling, providing added security for setting up reactions at room temperature. The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. Note that to ensure full activation of the WarmStart Luna RT, incubation at temperatures lower than 50°C is not recommended. This formulation contains no reference dye and is compatible with any instrument that does not require ROX (if ROX normalization is needed, ROX can be added.)
Alkali Scientific Radiant™ Green Lo-ROX qPCR Kit, 100 Reactions, (1 x 1mL)
Alkali Scientific offers Radiant™ Green Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Green Lo-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.
Alkali Scientific Radiant™ Green Lo-ROX qPCR Kit, 2,000 Reactions, (20 x 1mL)
Alkali Scientific offers Radiant™ Green Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Green Lo-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.
Alkali Scientific Radiant 1-Step SYBR Green Lo-ROX qPCR Kit, 1200 Reactions, (12 x 1mL 2X Green 1-Step LO-ROX Mix, 12 x 0.2mL 20X RTase for qPCR 1-Step)
Radiant™ 1-Step SYBR Green Kit uses the latest developments in reverse transcriptase technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The qPCR is performed by Radiant™ Taq HS DNA Polymerase, resulting in robust RT-PCR performance.Radiant™ 1-Step SYBR Green Kit can be used to quantify any RNA template including mRNA, total RNA and viral sequences. Extremely low copy number targets can be detected specifically with high efficiency. Proprietary small molecular inhibitor technology prevents formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest advancements in polymerase technology and advanced buffer chemistry, we offer market leading performance with minimal or no optimization.Many modern real-time PCR instruments have a fast cycling mode.
Alkali Scientific Radiant™ Probe Lo-ROX qPCR Kit, 500 Reactions, (5 x 1mL)
Alkali Scientific offers Radiant™ Probe Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in-class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low-copy assays and PCR conditions conducive to ultra-fast amplification. Furthermore, Radiant™ Probe Lo-ROX qPCR Kits are optimized for advanced multiplexing capability, delivering the same high efficiency, early Ct, and reaction speed even in complex multiplex assays.
Alkali Scientific Radiant™ Probe Hi-ROX qPCR Kit, 10,000 Reactions, (100 x 1mL)
Radiant™ Probe Hi-ROX qPCR Kit, 10,000 Reactions, (100 x 1mL)
Alkali Scientific Radiant Green Flourescein qPCR Kit, 100 Reactions, (1 x 1mL)
Radiant Green Flourescein qPCR Kit, 100 Reactions, (1 x 1mL)
Alkali Scientific Radiant™ Probe 1-Step Lo-ROX qPCR Kit, 300 Reactions, (3 X 1mL Probe 1-Step Lo-ROX 2X qPCR Mix, 3 x 0.2mL 20X RTase with RNase Inhibitor)
Alkali Scientific offers Radiant™ Probe 1-Step Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Probe 1-Step Lo-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.
Alkali Scientific Radiant 1Step SYBR Green LoROX qPCR Kit, 100 Reactions, (1 x 1mL 2X Green 1Step LOROX Mix, 1 x 0.2mL 20X RTase for qPCR 1Step)
Radiant 1Step SYBR Green LoROX qPCR Kit, 100 Reactions, (1 x 1mL 2X Green 1Step LOROX Mix, 1 x 0.2mL 20X RTase for qPCR 1Step)
Alkali Scientific Radiant™ Probe 1-Step Hi-ROX qPCR Kit, 300 Reactions, (3 X 1mL 2X Probe 1-Step Hi-ROX qPCR Mix, 3 X 0.2mL 20X RTase with RNase inhibitor)
Alkali Scientific offers Radiant™ Probe 1-Step Hi-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Probe 1-Step Hi-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.
Alkali Scientific Radiant™ qPCR Genotyping Mix, No-ROX, Suitable for Dual-labeled Taqman™ and Other Probes, 100 Reactions, (1 X 1mL)
Using the latest advancements in polymerase technology and advanced buffer chemistry Radiant™ Genotyping Mix No-Rox offers market leading performance with minimal optimization. Radiant™ Genotyping Mix No-Rox is a kit designed for use in dual-labeled probe based genotyping assays including Taqman, Molecular Beacon and Scorpion probe genotyping. Radiant™ GenotypingMix is fully compatible with ABI TaqMan Pre-Designed SNP Genotyping assays. Radiant™ Genotyping Mix uses proprietary small molecular inhibitor technology that prevents formation of primer-dimers to improve reaction sensitivity and specificity. Highthroughput screening has resulted in a buffer system that allows efficient amplification from GC-rich and AT-rich templates, under fast and standard cycling conditions.
Teknova 10 x TBE DNA Sequencing Grade Solution. 4 Liters.
890mM Tris, 890mM Boric Acid, 20mM Ethylenediaminetetraacetic acid (EDTA)
Alkali Scientific Radiant™ Probe No-ROX qPCR Kit, 5,000 Reactions, (50 x 1mL)
Radiant™ Probe No-ROX qPCR Kit, 5,000 Reactions, (50 x 1mL)