Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
NEW ENGLAND BIOGROUP 10 boxes of 50 LUNA-FX7 or LUNA-BX7 3-channel ultra low fluorescent counting slides, 500 slides/1500 counts. For LUNA-FX7 and LUNA-BX7.
10 boxes of 50 LUNA-FX7 or LUNA-BX7 3-channel ultra low fluorescent counting slides, 500 slides/1500 counts. For LUNA-FX7 and LUNA-BX7.
NEW ENGLAND BIOGROUP 1 box of 50 LUNA-FX7 or LUNA-BX7 1-channel ultra low fluorescent counting slides, 50 slides/50 counts. For LUNA-FX7 and LUNA-BX7.
1 box of 50 LUNA-FX7 or LUNA-BX7 1-channel ultra low fluorescent counting slides, 50 slides/150 counts. For LUNA-FX7 and LUNA-BX7.
Zymo Research Corporation ZymoTaq qPCR Premix (50 Rxns)
It features a hot-start DNA polymerase and buffer system optimized for the amplification of bisulfite-treatedDNA. It also includes an intense dsDNA-specific fluorescentdye, SYTO 9, for sensitive real-time DNA quantification. This technology can also be found in the Femto Quantification Kits (Cat. No. E2005, E2006, E2007) for low copy detection of DNA from human, bacteria, or fungi.
NEW ENGLAND BIOGROUP LUNA-FL automated cell counter fluorescent calibration beads. 1 pk/50 tests. .5 mL.
LUNA-FL automated cell counter fluorescent calibration beads. 1 pk/50 tests. .5 mL.
NEW ENGLAND BIOGROUP LUNA Acridine Orange Propidium Iodide, sterile filtered, 2 x .5mL. For use with LUNA-FL and LUNA-FX7 automated cell counters.
LUNA Acridine Orange Propidium Iodide, sterile filtered, 2 x .5mL. For use with LUNA-FL and LUNA-FX7 automated cell counters.
Sciencell Research Laboratories Human Ovarian Surface Epithelial Cells, Catalog #7310, Frozen, >5 x 10^5 cells in 1 ml
Human ovarian surface epithelial cells (HOSEpiC) are primary cells derived from human ovarian tissue and are vital for various research in ovarian physiology. Ideal for studying follicular dynamics, hormone expression, and the underlying mechanisms in ovarian cancers, these cells provide significant insights into cell survival and transformation processes.
- Isolated from human ovary
- Cryopreserved at early passage
- Delivered in frozen state
- Contains >5 x 10^5 cells in 1 ml
- Characterization markers include Cytokeratin-18 and/or Cytokeratin-19
- Negative pathogen testing for HIV-1, HBV, HCV, and others
- Guaranteed expansion of 5 population doublings
- Recommended medium is Ovarian Epithelial Cell Medium (OEpiCM)
- For research use only
Takara Bio PRIMESCRIPT RT REAGENT KIT
RR047A PrimeScript(TM) RT Reagent Kit with gDNA Eraser (Perfect Real Time) 100 Rxns
Norgen Biotek Corp 2X One-Step Rt-Pcr Mas 100Rxns
Norgen’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, and a PCR enhancer/stabilizer. The user needs only to add the template, the primer set and water to the Master Mix to set up the RT-PCR reaction. This convenient 2X One-Step RT-PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of RNA templates with high yields of PCR products.Taq Source: An E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1RT Source: An E. coli strain with a cloned reverse transcriptase gene from M-MuLV.
Cayman Chemical Nonpolar LIpId MIxture 2pl 1mL
A mixture of non-polar lipids; contains cholesteryl oleate, oleic acid methyl ester, 1,2,3-trioleoyl-rac-glycerol, oleic acid, and cholesterol; intended for use as a reference standard for the detection of these non-polar lipids in various sample types by TLC, LC-, or GC-MS
Alkali Scientific Radiant™ Probe Hi-ROX qPCR Kit, 500 Reactions, (5 x 1mL)
Alkali Scientific offers Radiant™ Probe Hi-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in-class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low-copy assays and PCR conditions conducive to ultra-fast amplification. Furthermore, Radiant™ Probe Hi-ROX qPCR Kits are optimized for advanced multiplexing capability, delivering the same high efficiency, early Ct, and reaction speed even in complex multiplex assays.
Alkali Scientific Radiant™ Green Lo-ROX qPCR Kit, 2,000 Reactions, (20 x 1mL)
Alkali Scientific offers Radiant™ Green Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Green Lo-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.
Alkali Scientific Radiant Green Lo-ROX qPCR Kit, 5,000 Reactions, (50 x 1mL)
Alkali Scientific offers Radiant™ Green Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Green Lo-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.
Alkali Scientific Radiant™ Green Lo-ROX qPCR Kit, 500 Reactions, (5 x 1mL)
Alkali Scientific offers Radiant™ Green Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Green Lo-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.
Alkali Scientific Radiant™ Probe Lo-ROX qPCR Kit, 2,000 Reactions, (20 x 1mL)
Alkali Scientific offers Radiant™ Probe Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in-class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low-copy assays and PCR conditions conducive to ultra-fast amplification. Furthermore, Radiant™ Probe Lo-ROX qPCR Kits are optimized for advanced multiplexing capability, delivering the same high efficiency, early Ct, and reaction speed even in complex multiplex assays.