Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Applied Biosystems™ TrueMark™ Comprehensive Microbiota Control
The TrueMark Comprehensive Microbiota Control contains a linearized multi-target plasmid pool with microbial target sequences commonly used on Custom OpenArray panels. The control has targets relevant to many microbial profiling applications, including respiratory tract, urinary tract, vaginal microbiota, and antibiotic resistance research.
Applied Biosystems™ TrueMark™ STI Select Panel Combo Kit
The Applied Biosystems TrueMark STI Select Panel Combo Kit is a multiplex real‐time PCR solution that contains the assay and master mix needed for the simultaneous detection of RNA in extracted human samples.
Applied Biosystems™ VetMAX™ Xeno™ Internal Positive Control - VIC™ Assay
The VetMAX™ Xeno™ Internal Positive Control (IPC) - VIC™ Assay is a primer-probe mix that detects the Xeno internal positive control. The resultant Xeno data is used to determine the validity of diagnostic test results.
Applied Biosystems™ VetMAX™ PRRSV 3.0 Reagents
VetMAX™ PRRSV 3.0 Reagents contain master mix, Xeno RNA control, and a primers/probes mix that enables multiplex, one-step, reverse transcription and real-time PCR (RT-PCR) assays targeting North American (NA) and European (EU) porcine reproductive and respiratory syndrome virus (PRSSV) RNA and Xeno RNA.
Applied Biosystems™ VetMAX™ PRRSV 3.0 Controls
VetMAX™ PRRSV 3.0 Controls are for use in multiplex, real-time reverse transcription PCR (RT-PCR) assays targeting North American and European porcine reproductive and respiratory syndrome virus (PRRSV) and Xeno RNA.
Applied Biosystems™ Detection Enhancer, for real-time PCR
Formulated to improve template amplification in real-time PCR (qPCR) workflows
Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
Invitrogen™ Collibri™ Library Quantification Kit
Invitrogen™ Collibri™ Library Quantification Kit includes a ready-to-use master mix optimized for Illumina NGS library quantification and a library dilution buffer
Applied Biosystems™ TrueMark™ PGx SNP + CNV 64 Panel, 384-well plate
The TrueMark PGx SNP + CNV 64 Panel is a pre-spotted 384-well PCR plate that is easy-to-use for low to mid-throughput pharmacogenomics research sample testing applications. The panel includes 56 SNP assays and 2 CNV assays.
Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents
TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe.
Applied Biosystems™ TaqPath™ qPCR Master Mix, CG
TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.
Applied Biosystems™ TrueMark™ Amplification Control I (1 x 105 copies/μL)
The TrueMark Amplification Control I for TrueMark plates contains a linearized multi-target plasmid pool with microbial target sequences used on TrueMark infectious disease research panels.
Applied Biosystems™ TaqMan™ Fast Reagent Starter Kit
Designed to detect and quantify Eukaryotic 18S rRNA sequence in cDNA samples using the TaqMan 5' nuclease assay, in fast thermal cycling mode.
Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit
Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.