Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Roche Diagnostics DNA Standards (0 - 6) only, 6 x 80 uL
DNA Standards (0 - 6) only, 6 x 80 uL. *This item is non returnable.
New England Biolabs, Inc. Luna® Universal Probe One-Step RT-qPCR Kit – 2500 reactions
In the Luna Universal One-Step Probe RT-qPCR Kit, Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling, providing added security for setting up reactions at room temperature. The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. Note that to ensure full activation of the WarmStart Luna RT, incubation at temperatures lower than 50°C is not recommended.
- Non-interfering, visible tracking dye helps to eliminate pipetting errors
As One International Inc ROX internal reference dye, 3 x 0.2 ml
A passive reference dye to compensate for non-PCR related variations in the fluorescence. The fluorescence from the passive reference dye does not change during the course of the PCR reaction but provides a stable baseline to which samples are normalized. The excitation and emission of the reference dye are 584 nm and 612 nm, respectively.
Agilent Technologies Brilliant II QRT-PCR Master Mix, 1-Step, 400 reactions, 25 μl/reaction, Moloney-based RT, 50°C, 5.5 mM MgCl2, multiplexing: true, storage: -20°C, stability: 6 months
Brilliant II QRT-PCR Master Mix, 1-Step, 400 reactions, 25 μl/reaction, Moloney-based RT, 50°C, 5.5 mM MgCl2, multiplexing: true, storage: -20°C, stability: 6 months
Alkali Scientific Radiant™ Green Lo-ROX qPCR Kit, 10,000 Reactions, (100 x 1mL)
Alkali Scientific offers Radiant™ Green Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Green Lo-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.
Roche Diagnostics HotStart ReadyMix with dye | 100x25uL Reactions
KAPA2G Fast PCR Kits contain a second-generation (2G) DNA polymerase enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-typeTaqpolymerase. In addition to speed, KAPA2G Fast PCR Kit provides higher yields and sensitivity than competitor enzymes across a broad range of targets. *This item is non returnable.
Alkali Scientific Radiant 1-Step SYBR Green Lo-ROX qPCR Kit, 1200 Reactions, (12 x 1mL 2X Green 1-Step LO-ROX Mix, 12 x 0.2mL 20X RTase for qPCR 1-Step)
Radiant™ 1-Step SYBR Green Kit uses the latest developments in reverse transcriptase technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The qPCR is performed by Radiant™ Taq HS DNA Polymerase, resulting in robust RT-PCR performance.Radiant™ 1-Step SYBR Green Kit can be used to quantify any RNA template including mRNA, total RNA and viral sequences. Extremely low copy number targets can be detected specifically with high efficiency. Proprietary small molecular inhibitor technology prevents formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest advancements in polymerase technology and advanced buffer chemistry, we offer market leading performance with minimal or no optimization.Many modern real-time PCR instruments have a fast cycling mode.
Alkali Scientific Radiant™ Probe Lo-ROX qPCR Kit, 100 Reactions, (1 x 1mL)
Alkali Scientific offers Radiant™ Probe Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in-class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low-copy assays and PCR conditions conducive to ultra-fast amplification. Furthermore, Radiant™ Probe Lo-ROX qPCR Kits are optimized for advanced multiplexing capability, delivering the same high efficiency, early Ct, and reaction speed even in complex multiplex assays.
Andwin Scientific REAL-TIME PCR STRIP TUBES
The 0,2 mL GeneMate SnapStrip 8-Strip Standard PCR Tubes feature individually attached flat caps that helps in the transmission and also acts as a writing or marking surface. The caps also have a high clarity manufacturing that is ideal for real-time PCR (QPCR) applications where excitation and emission takes through the cap. The tubes feature great compatibility with a wide range of thermal cyclers.