Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
New England Biolabs, Inc. Luna Universal Probe qPCR Master Mix – 1000 rxn (10 x 1 ml)
The NEB Luna Universal Probe qPCR Master Mix is a 2X reaction mix optimized for real-time qPCR detection and quantitation of target DNA sequences using hydrolysis probes. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap fluorophores commonly used for qPCR and will not interfere with real-time detection. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers/probes and DNA template. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR and existing as well as commercial qPCR assay primer/probe sequences can be used.
ACROBiosystems E. coli resDNA Quantitative Kit (qPCR)
Residual host cell DNA refers to fragments of DNA from the host organism that are left behind after a biological process such as the production of a biopharmaceutical product or the cultivation of a cell line. This residual DNA can potentially contaminate the final product and affect its safety and efficacy. In the context of biopharmaceutical production, regulatory agencies such as the FDA and EMA have set limits on the amount of residual host cell DNA that is acceptable in a final product. These limits vary depending on the type of product and the route of administration, and residue host cell DNA quantitative kits are designed to ensure that the final product is safe for human use.
Takara Bio EMERALDAMP GT 800 RXN
PCR Master Mix, EmeraldAmp; 800 x 50uL rxns; Includes optimized buffer, PCR enzyme, dNTP mixture, gel loading dye (green) and density reagent in 2X premix
Takara Bio SAPPHIREAMP MASTER MIX 160RXN
MasterMix, PCR; Takara; SapphireAmp Fast PCR Master Mix; 160rxns
Alkali Scientific Radiant™ Probe No-ROX qPCR Kit, 10,000 Reactions, (100 x 1mL)
Radiant™ Probe No-ROX qPCR Kit, 10,000 Reactions, (100 x 1mL)
Alkali Scientific Radiant™ Probe 1-Step No-ROX qPCR Kit, 100 Reactions, (1 X 1mL 2X Mix, 1 X 0.2mL 20X RTase)
Alkali Scientific offers Radiant™ Probe 1-Step No-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Probe 1-Step No-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.
Alkali Scientific Radiant™ Probe 1-Step Hi-ROX qPCR Kit, 300 Reactions, (3 X 1mL 2X Probe 1-Step Hi-ROX qPCR Mix, 3 X 0.2mL 20X RTase with RNase inhibitor)
Alkali Scientific offers Radiant™ Probe 1-Step Hi-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Probe 1-Step Hi-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.
Enzo Life Sciences AMPIGENE qPCR 1-Step Green Kit Hi-ROX (1000Reactions)
AMPIGENE qPCR 1-Step Green Kits use the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active. AMPIGENE qPCR 1-Step Green Kits use a proprietary intercalating dye that does not inhibit PCR. An optimized buffer system allows efficient amplification and proprietary enhancers prevent the formation of primers/dimers to increase sensitivity and specificity. ROX is a reference dye which is used to normalize fluorescent signal in qPCR. AMPIGENE qPCR 1-Step Green Kits come with either Lo-ROX or Hi-ROX concentration. For Lo-ROX, see ENZ-NUC108. Choose the appropriate ROX concentration for the instrument (see instrument compatibility for reference dye chart below. Applications: PCR.
Enzo Life Sciences AMPIGENE qPCR 1-Step Probe Kit No-ROX (200Reactions)
AMPIGENE qPCR 1-Step Probe Kits use the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active. AMPIGENE qPCR 1-Step Probe Kits are designed for use on a variety of probes including: Taqma , Molecular Beacons, and Scorpion probes. An optimized buffer system allows efficient amplification and proprietary enhancers prevent the formation of primers/dimers to increase sensitivity and specificity. ROX is a reference dye which is used to normalize fluorescent signal in qPCR. AMPIGENE qPCR 1-Step Probe Kits come with either Lo-ROX, Hi-ROX, or No-ROX concentration. Choose the appropriate ROX concentration for the instrument (see instrument compatibility for reference dye chart below.) Applications: PCR.
Alkali Scientific Radiant™ Green Hi-ROX qPCR Kit, 100 Reactions, (1 x 1mL)
Alkali Scientific offers Radiant™ Green Hi-ROX qPCR Kits, a high-performance 2X ready-to-use real-time PCR reagents designed for best-in-class quantitative PCR. Radiant™ Green Hi-ROX qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low-copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Green Hi-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection in combination with fluorescein for optional use with certain qPCR instruments.
Enzo Life Sciences EPIXTRACT DNA Isolation Kit for Plasma/Serum (50Reactions)
EPIXTRACT DNA Isolation Kit for Plasma/Serum provides a simple method to isolate DNA from plasma, serum, and body fluids. Assay Time: <15 min.
Alkali Scientific Radiant™ Probe Lo-ROX qPCR Kit, 100 Reactions, (1 x 1mL)
Alkali Scientific offers Radiant™ Probe Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in-class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low-copy assays and PCR conditions conducive to ultra-fast amplification. Furthermore, Radiant™ Probe Lo-ROX qPCR Kits are optimized for advanced multiplexing capability, delivering the same high efficiency, early Ct, and reaction speed even in complex multiplex assays.
System Biosciences LLC GLOBAL ULTRARAPID LENTIVIRAL
UltraRapid Lentiviral Global Titering Kit (Human and Mouse compatible) 42 reactions