Additional Selective and Differential Cell Culture Media
NEOGEN Pseudomonas Agar Base, 500 g
Pseudomonas Agar Base is used for the selective and differential isolation of Pseudomonas sp. using CN or CFC supplementation and is not intended for use in the diagnosis of disease or other conditions in humans. Pseudomonas Agar Base was developed to be supplemented with CN X107 (Cetrimide & Nalidixic Acid) as per ISO 16266-2:2018, or with CFC X108 (Cetrimide, Fusidate, & Cephaloridine) as per ISO 13720:2010. This base formula is a modification of King’s A Medium. The CN Supplement is recommended for the isolation of Pseudomonas aeruginosa, where the addition of Nalidixic Acid and the reduction of Cetrimide improved recovery.For more information visit https://www.neogen.com/solutions/Microbiology/pseudomonas-agar-base
NEOGEN Mueller Hinton Agar I, 500 g
Mueller Hinton Agar I is used for antimicrobial sensitivity testing by the disc diffusion method and is not intended for use in the diagnosis of disease or other conditions in humans.A medium for antimicrobial sensitivity testing by the disc diffusion method. This medium, used in the technique of Bauer and Kirby, has been adopted by the National Committee for Clinical Laboratory Standards (NCCLS) in the USA as the definitive method for susceptibility testing. The medium has a very low thymine and thymidine content, making it suitable for trimethoprim and sulphonamide testing, controlled to ensure correct zone sizes with aminoglycoside and tetracyline antibiotics. The medium was originally formulated as a heat labile protein free medium for the isolation of pathogenic Neisseriaceae.For more information visit https://www.neogen.com/solutions/Microbiology/mueller-hinton-agar-1
NEOGEN Letheen Agar Base Modified without Tween, 500 g
Letheen Agar Base Modified (without Tween) is used with Tween 80 (Polysorbate 80) for the isolation of microorganisms from cosmetics in a laboratory setting. Letheen Agar Base Modified is not intended for use in the diagnosis of disease or other conditions in humans.In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Tween 80 (Polysorbate 80) to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds in testing of germicidal activity. Total neutralization of disinfectants is critical. Disinfectant residues can result in a false negative (no-growth) test.For more information visit https://www.neogen.com/solutions/Microbiology/letheen-agar-base-modified-without-tween
Promocell GmbH PromoCell Supplement Mix / Small Airway Epithelial Cell Growth Medium
PromoCell Airway Epithelial Cell Growth Medium is a serum-free medium optimized for the in vitro cultivation of epithelial cells from large air passages.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for equine, porcine, murine, bat and rat airway epithelial cells.To view a list of references where this medium was used with other species/cell types please check manufacturer homepage.| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.5 μg / ml || Epinephrine | 0.5 μg / ml || Triiodo-L-thyronine | 6.7 ng / ml || Transferrin (recombinant human) | 10 μg / ml || Retinoic Acid | 0.1 ng / ml |
Lonza Walkersville Hepatocyte Plating Medium
Hepaotcyte Plating Medium for ensuring optimal plating efficiency of primary human and animal hepatocytes.Benefits:Optimized for Hepatocyte Cell healthApplications:ADMEToxContent:100 mL Basal medium2 mL supplement
Lonza Walkersville Hepatocyte P.M w/Splmnt(MP250-1+MP250-2)
Hepatocyte P.M w/Splmnt(MP250-1+MP250-2)
Promocell GmbH PromoCell Fibroblast Growth Medium 500 ml, ready-to-use, serum-free, with C-39315 attached to the flask
PromoCell Fibroblast Growth Medium is optimized for the cultivation of neonatal and juvenile fibroblasts.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, and rat fibroblasts as well as fibroblast cell lines.While the supplements of the Fibroblast Growth Medium are serum-free, the Basal Medium is manufactured with 0.5% serum.| Media supplement | Final supplement concentration||----------------------------------------------------|---------------------------------------------------------------|| Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 5 μg / ml |
NEOGEN Palcam Agar Base, 5 kg
Palcam Agar Base is used with supplements as a selective and differential medium for the detection and isolation of Listeria monocytogenes from food and environmental samples. Palcam Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. Palcam Agar was developed by Van Netten et al in 1989 as an improved selective differential medium for the isolation of Listeria monocytogenes from food and environmental specimens. Improved selectivity is achieved by the combination of antibiotic supplements and microaerobic incubation. The double indicator system of esculin hydrolysis and mannitol fermentation aids differentiation of Listeria spp from enterococci and staphylococci which can be confused with Listeria spp on other types of culture media. Palcam Agar is listed within Annex E of ISO 11290-1:2017 as a suitable second selective agar.For more information visit https://www.neogen.com/solutions/Microbiology/palcam-agar-base
NEOGEN m-Endo Agar, 500 g
m-Endo Agar is used for the enumeration of coliforms in water by the membrane filtration method in a laboratory setting. m-Endo Agar is not intended for use in the diagnosis of disease or other conditions in humans.The coliform group, especially Escherichia coli, are used as indicators of fecal pollution in water, for assessing the effectiveness of water treatment and disinfection, and for monitoring water quality. m-Endo Agar is prepared according to the formula of McCarthy, Delaney, and Grasso, and used in testing water for coliforms by a two-step membrane filtration procedure. Lauryl Tryptose Broth is used as the preliminary enrichment, resulting in higher coliform counts.The American Public Health Association (APHA) recommends m-Endo Agar in standard total coliform membrane filtration procedure for testing water, wastewater, and foods.For more information visit https://www.neogen.com/solutions/Microbiology/m-endo-agar
NEOGEN APT Agar, 500 g
APT Agar is used for the cultivation of heterofermentative lactobacilli in a laboratory setting. APT Agar is not intended for use in the diagnosis of disease or other conditions in humans.Evans and Niven investigated the cultivation of heterofermentative lactobacilli, causing the faded or green discoloration of cured meat products. Deibel, Evans, and Niven tested thiamine-requiring bacteria, specifically Lactobacillus viridescens. Their formulations led to the development of APT Agar.Lactic acid bacteria, a group of acid-producing bacteria, include the genera Streptococcus, Leuconostoc, Pediococcus, and Lactobacillus. These organisms are widespread in nature, associated with bacterial spoilage of foods including dairy products, meat, and vegetables. APT Agar is used for cultivating heterofermentative lactic acid bacteria from food products.For more information visit https://www.neogen.com/solutions/Microbiology/apt-agar
NEOGEN Potato Dextrose Agar, 500 g
Potato Dextrose Agar is used for the preparation and maintenance of fungal test strains used in the growth promotion test, suitability of the counting methods and negative controls as described in the Harmonized USP/EP/JP. Potato Dextrose Agar is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized USP/EP/JP for the cultivation of fungi and specifically for the preparation of the Aspergillus brasiliensis test strain. Conforms to USP/EP/JP performance specification. The medium is commonly abbreviated to PDA. The extract from potato and dextrose provide a nutritionally rich base that encourages mold sporulation and pigment production. For more information visit https://www.neogen.com/solutions/Microbiology/potato-dextrose-agar
NEOGEN Violet Red Bile Agar (VRBA) (ISO), 500 g
A medium for the enumeration of coliform organisms in food and dairy products according to ISO 4832:2006 and is not intended for use in the diagnosis of disease or other conditions in humans.The selectivity of the medium is due to the presence of bile salts and crystal violet. Lactose fermenters produce red/purple colonies often surrounded by a halo of the same color. Non-lactose fermenters produce pale colonies. Selectivity can be increased by incubation at 42-44°C.Formula:Yeast Extract - 3.0 g/LEnzymatic Digest of Animal Tissue - 7.0 g/LBile Salts - 1.5 g/LLactose - 10.0 g/LNeutral Red - 0.03 g/LCrystal Violet - 0.002 g/LAgar- 12.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/violet-red-bile-lactose-agar-iso
NEOGEN Bile Esculin Azide Agar, 500 g
Bile Esculin Azide Agar is used for the selective isolation and differentiation of group D streptococci in a laboratory setting. Bile Esculin Azide Agar is not intended for use in the diagnosis of disease or other conditions in humans.Bile Esculin Azide Agar is a modification of the medium reported by Isenberg and Isenberg, Goldberg, and Sampson. This formula modifies Bile Esculin Agar by adding sodium azide and reducing the concentration of bile. The revised medium is more selective, but still provides rapid growth and efficient recovery of group D streptococci.Molecular taxonomic studies of the genus Streptococcus have placed enterococci, previously described group D streptococci, in the genus Enterococcus. The ability to hydrolyze esculin in the presence of bile is a characteristic of enterococci and group D streptococci.For more information visit https://www.neogen.com/solutions/Microbiology/bile-esculin-azide-agar