Additional Selective and Differential Cell Culture Media
NEOGEN Universal Pre-enrichment Broth, 5 kg
Universal Pre-enrichment Broth is used for the recovery of Salmonella spp. and Listeria spp. in a laboratory setting. Universal Pre-enrichment Broth is not intended for use in the diagnosis of disease or other conditions in humans. Traditional methods for recovering Salmonella spp. and Listeria spp. from food products require separate pre-enrichment media for each microorganism. Some broth media recommended for preenrichment contain antibiotic inhibitors or have insufficient buffering capacity which hinder recovery of sublethally injured cells. Bailey and Cox formulated Universal Preenrichment Broth to permit simultaneous resuscitation of sublethally injured Salmonella and Listeria. The broth medium provides sufficient buffering capacity to prevent rapid decreases in pH and allows for repair of injured cells that might be sensitive to low pH values or inhibitory substances.For more information visit https://www.neogen.com/solutions/Microbiology/universal-preenrichment-broth
NEOGEN XLT4 Agar, 5 kg
XLT4 Agar is used with Tergitol 4 for the isolation of non-typhi Salmonella spp and is not intended for use in the diagnosis of disease or other conditions in humans. XLT4 Agar is a selective differential isolation medium for the specific detection of Salmonella spp. from environmental and food samples. Due to its highly selective nature, XLT4 Agar is particularly effective when used with samples where overgrowth of contaminating flora is expected, for example, fecally contaminated agricultural samples. Developed to perform as per Miller & Tate in 1990, this medium was found to improve the recovery of non-typhi Salmonella from chicken and farm environmental samples. Dusch and Altwegg further established the application of XLT4 Agar to salmonellae detection in clinical samples, with the notable exceptions of Salmonella typhi and Salmonella paratyphi.For more information visit https://www.neogen.com/solutions/Microbiology/xlt4-agar
NEOGEN Violet Red Bile Agar with MUG (VRBA-MUG), 5 kg
Violet Red Bile Agar with MUG is used for the detection of coliforms and the fluorogenic detection of Escherichia coli. Violet Red Bile Agar with MUG is not intended for use in the diagnosis of disease or other conditions in humans. Violet Red Bile Agar W/ MUG is specified in many procedures to enumerate coliforms in food and dairy products. In 1982, Feng and Hartman developed a rapid fluorogenic assay for Escherichia coli by incorporating 4-methylumbelliferyl-β-D-glucuronide (MUG) into Lauryl Tryptose Broth. Similarly, incorporating MUG into Violet Red Bile Agar permits the detection of E. coli among the coliform colonies. Standard methods procedures specify Violet Red Bile Agar with MUG for detecting E. coli in food and dairy products by fluorescence. This medium is often abbreviated as VRBA w/ MUG.For more information visit https://www.neogen.com/solutions/Microbiology/violet-red-bile-agar-with-mug
NEOGEN Brilliant Green Agar with Sulfapyridine, 5 kg
Brilliant Green Agar w/ Sulfapyridine is used for the selective enrichment of Salmonella spp. in a laboratory setting. Brilliant Green Agar w/ Sulfapyridine is not intended for use in the diagnosis of disease or other conditions in humans.Brilliant Green Agar was first described by Kristensen et al and later modified by Kauffmann. The outstanding selectivity of this medium permits the use of moderately heavy inocula evenly distributed over the surface. The addition of sulfonamides into Brilliant Green Agar further inhibits Escherichia coli and Proteus spp. Osborne and Stokes used 0.1% Sodium Sulfapyridine to enhance the recovery of Salmonella from whole egg and egg yolk.For more information visit https://www.neogen.com/solutions/Microbiology/brilliant-green-agar-with-sulfapyridine
NEOGEN Buffered Peptone Water (BPW), 5 kg
Buffered Peptone Water (BPW) is used for the non-selective pre-enrichment of Salmonella spp. from food and is not intended for use in the diagnosis of disease or other conditions in humans. Edel and Kamelmacher found that food preservation techniques involving heat, desiccation, preservatives, high osmotic pressure, or pH changes cause sublethal injury to Salmonella spp. Preenrichment in a non-selective medium allows for repair of cell damage and facilitates the recovery of Salmonella. Lactose Broth is frequently used for this purpose, but it may be detrimental to recovering Salmonellae. Buffered Peptone Water maintains a high pH over the pre-enrichment period and allows in repair of injured cells that may be sensitive to low pH. This is particularly important for vegetable specimens which have a low buffering capacity. Buffered Peptone Water is used in standard methods. For more information visit https://www.neogen.com/solutions/Microbiology/buffered-peptone-water
Bioworld Lindemann Orchid Medium, 50 L
Lindemann Orchid MediumContains the Macronutrients, Micronutrients, Sucrose, and Vitamins as described by Lindemann et al. (1970)Cytokines and Auxins should be added as needed Provided as 1 unit to make 25L or 50L of medium, or as 10 units to make 1L of mediumPlant tissue culture testedWorking Concentration: 22.71 g/LSoluble in water
Bioworld Litvay Medium, 50 L
Litvay MediumContains Macronutrients and Micronutrients as described by Litvay et al. (1985) Sucrose, Cytokines, Vitamins, and Auxins should be added as needed Provided as 1 unit to make 10L, 25L, or 50L of medium, or as 10 units to make 1L of medium Plant tissue culture tested
Bioworld Murashige & Miller Medium, 50 L
Murashige & Miller MediumContains Micronutrients, Macronutrients, and Vitamins as described by Murashige andSkoog (1962)Syngonium Stage I and II Sucrose, Cytokines, and Auxins should be added as needed Provided as 1 unit to make 10L, 25L, or 50L of medium, or as 10 units to make 1L of mediumPlant tissue culture tested
Bioworld Schenk & Hildebrandt Modified Salt Mixture w/o CaCl2, 25 L
Schenk & Hildebrandt Modified Salt MixtureContains the Macronutrients and Micronutrients as described by Schenk & Hildebrandt (1972)Does not contain Calcium ChlorideSucrose, Cytokines, Vitamins, and Auxins should be added as needed Provided as 1 unit to make 25L or 50L of medium, or as 10 units to make 1L of mediumPlant tissue culture testedWorking Concentration: 3.05 g/LSoluble in water
Bioworld Wheat Regeneration Medium (MMS2C), 50 L
Wheat Regeneration Medium (MMS2C)Regeneration medium for monocotsContains MS basal salts with vitamins, sucrose and agar to be supplemented with specific growth regulators.Add 0.001 g/L IAA and 0.002 g/L 6-BAP after autoclavingProvided as 1 unit to make 10L, 25L, or 50L of medium, or as 10 units to make 1L of mediumPlant tissue culture tested
Bioworld Murashige and Skoog(MS) Medium, Modification No. 4, 50 L
Murashige & Skoog (MS) MediumContains Macronutrients and Micronutrients with No NH4NO3 as described by Murashige & Skoog (1962)Sucrose, Cytokines, Vitamins, and Auxins should be added as neededProvided as 1 unit to make 10L, 25L, or 50L of medium, or as 10 units to make 1L of mediumPlant tissue culture testedWorking concentration 2.7 g/L
Zen Bio Inc CnT-Prime 2D Diff. (CnT-PR-D)
Media designed for proliferation and longevity contain a range of factors that are known to retard the differentiation process.The CnT-PR-D medium is formulated with a totally different balance of growth factors to encourage cells to differentiate quickly and efficiently in 2D culture.
ATCC 0.1% Gelatin Solution, 100ml, Sterile
0.1% Gelatin Solution is used to coat cell culture flasks. The use of coated flasks imporves cell attachment for certain tupes of primary cells as well as certain continuous cell lines. New ATCC customers are required to register with ATCC prior to purchase. The application can be found at https://www.atcc.org/en/My_Profile/Register.aspx