Additional Selective and Differential Cell Culture Media
NEOGEN Bacillus cereus MYP Agar, 500 g
Bacillus Cereus MYP Agar is used with supplements for the selective and differential enumeration of Bacillus cereus from foods. Conforms to ISO and FDA / BAM formulation in a laboratory setting. MYP Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.Formulated to ISO 7932:2004 MYP was introduced by Mossel and his co-workers in 1967 for the enumeration of Bacillus cereus in foods and was shown to be the most effective for this purpose by Inal in 1972. B. cereus is differentiated from other members of the Bacillus group by two reactions: mannitol fermentation and lecithinase production. Mannitol fermentation on this medium produces a yellow color. B. cereus is mannitol negative and produces red colonies. The lecithinase production of B. cereus is indicated by a white precipitate around the colonies.For more information visit https://www.neogen.com/solutions/Microbiology/bacillus-cereus-myp-agar
NEOGEN Bacteriological Peptone, 500 g
Bacteriological Peptone is for use in preparing microbiological culture media in a laboratory setting, and is not intended for use in the diagnosis of disease or other conditions in humans.An economical source of nutrients provided by a balanced mixture of meat peptones and tryptone. The growth requirements of most non-fastidious organisms will be fulfilled by the range of amino acids, peptides and proteoses in this mixture.For more information visit https://www.neogen.com/solutions/Microbiology/bacteriological-peptone
NEOGEN MacConkey Broth Purple, 500 g
MacConkey Broth Purple is used for the detection and enumeration of fecal coliforms (37°C) and E. coli (44°C). This medium is not intended for use in the diagnosis of disease or other conditions in humans.The replacement of neutral red used in the original formulation by bromocresol purple makes the color change caused by acid producing organisms easier to read.For more information visit https://www.neogen.com/solutions/Microbiology/macconkey-broth-purple
NEOGEN Harlequin Cronobacter Isolation Agar, 500 g
Chromogenic Cronobacter Isolation Agar (CCI) is recommended by ISO 22964 for the isolation and identification of Cronobacter spp. (formerly Enterobacter sakazakii), and is not intended for use in the diagnosis of disease or other conditions in humans.Although rarely causing infections in immunocompetent adults, members of the Cronobacter genus have been implicated in sepsis, meningitis and necrotizing enterocolitis with a high death rate in neonates. These opportunistic pathogens are common in the environment and their ability to survive desiccation presents a significant risk for post-pasteurization contamination and survival in spray dried milk products. Tryptic digest of casein and yeast extract provide a source of nitrogen and essential vitamins. The high content of sodium chloride and the presence of sodium desoxycholate produce a selective and specific medium.For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-cronobacter-isolation-agar
NEOGEN Sorbitol MacConkey Agar, 500 g
Sorbitol MacConkey Agar is used for the isolation of pathogenic Escherichia coli in a laboratory setting. Sorbitol MacConkey Agar is not intended for use in the diagnosis of disease or other conditions in humans. Sorbitol MacConkey Agar is based on the formula by Rappaport and Henig. Originally developed for isolating enteropathogenic (EPEC) serotypes, O11 and O55, this medium is recommended for the isolation and differentiation of enterohemorrhagic E. coli O157:H7. Serotype O157 has been implicated in serious foodborne diseases. MacConkey Agar W/ Sorbitol contains sorbitol instead of lactose for differentiating enteropathogenic E. coli serotypes; these strains are typically sorbitol negative. MacConkey Agar W/ Sorbitol is recommended for food testing.For more information visit https://www.neogen.com/solutions/Microbiology/sorbitol-macconkey-agar
NEOGEN Cronobacter Selective Broth, 500 g
Cronobacter Selective Broth (CSB) is recommended by ISO 22964 for the isolation and identification of Cronobacter spp. (formerly Enterobacter sakazakii), and is not intended for use in the diagnosis of disease or other conditions in humans.Although rarely causing infections in immunocompetent adults, members of the Cronobacter genus have been implicated in sepsis, meningitis and necrotizing enterocolitis with a high death rate in neonates. These opportunistic pathogens are common in the environment and their ability to survive desiccation presents a significant risk for post-pasteurization contamination and survival in spray dried milk products. Enzymatic digest of animal tissues and meat extract provide a source of nitrogen and essential vitamins.For more information visit https://www.neogen.com/solutions/Microbiology/cronobacter-selective-broth
NEOGEN Potato Dextrose Broth, 5 kg
Potato Dextrose Broth is used for the cultivation of fungi in a laboratory setting. Potato Dextrose Broth is not intended for use in the diagnosis of disease or other conditions in humans. Potato Dextrose Broth is a general purpose broth for yeasts and molds. The low pH of this medium inhibits bacterial growth. Potato Dextrose Broth is the same formula as Potato Dextrose Agar, but agar has been omitted.For more information visit https://www.neogen.com/solutions/Microbiology/potato-dextrose-broth
NEOGEN Cetrimide Agar
Cetrimide Agar is used in the isolation and identification of Pseudomonas aeruginosa and is not intended for use in the diagnosis of disease or other conditions in humans. Conforms to Harmonized USP/EP/JP Requirements. A medium recommended by the Harmonized USP/EP/JP for the isolation and identification of Pseudomonas aeruginosa, in non- sterile pharmaceutical samples. Conforms to USP/EP/JP performance specification. Gelatin is a source of nitrogen while glycerol acts as a carbon source. Cetrimide is a quaternary ammonium compound that inhibits the growth of a wide range of Gram-positive and some Gram-negative micro-organisms. Magnesium chloride and Dipotassium sulphate improve the production of pyoverdin and pyocyanin pigments that combine to give Pseudomonas aeruginosa characteristic green colonies.For more information visit https://www.neogen.com/solutions/Microbiology/cetrimide-agar
NEOGEN Tetrathionate (TT) Broth Base, Hajna, 5 kg
Tetrathionate (TT) Broth Base, Hajna is used as a selective enrichment for the cultivation of Salmonella species (except S. typhi) from samples in a laboratory setting. Tetrathionate (TT) Broth Base, Hajna is not intended for use in the diagnosis of disease or other conditions in humans. Tetrathionate Broth Base, Hajna, is recommended in the Compendium of Methods for the Microbiological Examination of Foods and the FDA/BAM for the Environmental Sampling and Detection of Salmonella Enteritidis (SE) in Poultry Houses and whole eggs.. Salmonella organisms may be present but injured in food-processing procedures, which include exposure to low temperatures, sub-marginal heat, drying, radiation, preservative, and sanitizers. Numerous reported cases of Salmonella enteritidis (SE) infections are associated with contaminated shell eggs.For more information visit https://www.neogen.com/solutions/Microbiology/tetrathionate-tt-broth-base-hajna
NEOGEN Rappaport Vassiliadis R10 Broth, 500 g
Rappaport Vassiliadis R10 Broth is used for the selective enrichment of Salmonella spp. from foods in a laboratory setting. Rappaport Vassiliadis R10 Broth is not intended for use in the diagnosis of disease or other conditions in humans. Rappaport et al formulated an enrichment medium for Salmonella spp. that was modified by Vassiliadis et al. The Rappaport formulation, designated R 25/37°C, recommended incubation at 37°C. The Vassiliadis modification, designated R 10/43°C, had a reduced level of Malachite Green and recommended incubation at 43°C. Peterz later showed that incubation at 41.5 ± 0.5°C for 24 hours improved recovery of Salmonella spp. Rappaport-Vassiliadis R10 Broth is a selective enrichment medium that is used following pre-enrichment of the specimen.For more information visit https://www.neogen.com/solutions/Microbiology/rappaport-vassiliadis-r10-broth
NEOGEN EC Medium with MUG, 500 g
EC Medium w/ MUG is used for the fluorogenic detection of Escherichia coli in a laboratory setting. EC Medium w/ MUG is not intended for use in the diagnosis of disease or other conditions in humans.EC Medium was developed by Hajna and Perry in an effort to improve the methods for the detection of the coliform group and E. coli. This medium consists of a buffered lactose broth with the addition of 0.15% Bile Salts Mixture. Growth of spore-forming bacteria is inhibited by the bile salts, while growth of E. coli is enhanced by its presence. EC Medium w/ MUG is the same formula as EC Medium, with the addition of 4-methylumbelliferyl-ß-D-glucuronide. Feng and Hartman developed a rapid assay for E. coli by incorporating 4-methylumbelliferyl-ß-D-glucuronide (MUG) into Lauryl Tryptose Broth at a final concentration of 100 ɥg/mL.For more information visit https://www.neogen.com/solutions/Microbiology/ec-medium-with-mug
NEOGEN LESS Plus Medium Irradiated, 500 g
Listeria monocytogenes, described first in 1926 by Murray, Webb and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis indicates the principle route of transmission is via the consumption of foods contaminated with Listeria monocytogenes. Implicated vehicles of transmission include meat, eggs, chicken, vegetables, and dairy products. Listeria spp. are ubiquitous in nature, present in a wide range of unprocessed foods and in soil, sewage, and river water. Certain strains of Listeria spp. are able to survive the manufacturing and ripening processes in dairy products.For more information visit https://www.neogen.com/solutions/Microbiology/less-plus-medium
NEOGEN A-1 Medium, 500 g
A-1 Medium is used for the detection of coliform organisms in water and food in a laboratory setting. A-1 Medium is not intended for use in the diagnosis of disease or other conditions in humans.Since the early 1900’s enumeration of coliform organisms, specifically E. coli, have been used to determine water purity. Elevated temperature and most-probable-number (MPN) methods are routinely used for analysis of water and food samples for presence of fecal coliforms. A-1 Medium was formulated to hasten E. coli recovery and reduce the incidence of false positive cultures.In 1972 Andrews and Presnell developed A-1 Medium. This medium recovers E. coli from estuarine water in 24 hours instead of 72 hours, and in greater numbers without the pre-enrichment step. A-1 Medium can be used in a single step procedure for the detection of fecal coliforms in source water, seawater, treated wastewater, and foods.For more information visit https://www.neogen.com/solutions/Microbiology/a-1-medium
NEOGEN Sabouraud Dextrose Agar, Emmons, 500 g
Sabouraud Dextrose Agar, Emmons is used for the cultivation of fungi in a laboratory setting. Sabouraud Dextrose Agar, Emmons is not intended for use in the diagnosis of disease or other conditions in humans. Sabouraud Dextrose Agar (SDA), Emmons is a modification of Dextrose Agar described by Sabouraud. SDA is used for cultivating pathogenic and commensal fungi and yeasts. Emmons modified the formula by adjusting the pH and reducing the dextrose concentration. This neutral pH enhances recovery of some pathogenic fungi. Sabouraud Dextrose Agar, Emmons is an excellent basal medium, and antibiotics and inhibitors may be added for the selective cultivation and isolation of various microorganisms.For more information visit https://www.neogen.com/solutions/Microbiology/sabouraud-dextrose-agar-emmons
NEOGEN XLT4 Agar, 500 g
XLT4 Agar is used with Tergitol 4 for the isolation of non-typhi Salmonella spp and is not intended for use in the diagnosis of disease or other conditions in humans. XLT4 Agar is a selective differential isolation medium for the specific detection of Salmonella spp. from environmental and food samples. Due to its highly selective nature, XLT4 Agar is particularly effective when used with samples where overgrowth of contaminating flora is expected, for example, fecally contaminated agricultural samples. Developed to perform as per Miller & Tate in 1990, this medium was found to improve the recovery of non-typhi Salmonella from chicken and farm environmental samples. Dusch and Altwegg further established the application of XLT4 Agar to salmonellae detection in clinical samples, with the notable exceptions of Salmonella typhi and Salmonella paratyphi.For more information visit https://www.neogen.com/solutions/Microbiology/xlt4-agar