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Liquid solutions, reagents, and dry compounds designed to support the growth, proliferation, and cryopreservation of insect cells; various formulations and volumes available.
A number of modifications of Graces medium were developed in several laboratories in the late 1960s and 1970s. Many of these modifications were developed by Dr. W.f. Hink for the growth of cells derived from the cabbage looper Tricoplusia ni. This formula represents the modification known as TNM-fH. The medium when properly supplemented has been found to support the growth of cells derived from a variety of lepidopteran species.
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Recombinant human polo-like kinase 1 (PLK1) expressed in Sf9 insect cells and supplied as a filtered solution with an N-terminal 10xHis tag. Intended for research applications including biochemical assays, antibody generation, and functional studies of mitosis-related signaling.
Expressed in Sf9 insect cells, enabling eukaryotic folding and glycosylation.
N-terminal 10xHis tag for affinity purification and detection.
Purity >95% by reducing SDS-PAGE.
Observed molecular weight ~66-74 kDa due to glycosylation.
Supplied as a 0.22 μm filtered solution in glycerol-containing buffer for stability.
Store at -80°C; stable at -20°C short term; avoid repeated freeze-thaw.
Suitable for kinase assays, antibody production, and cell signaling studies.
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Expression Systems offers insect cell lines adapted to serum-free suspension culture in either ESF 921 or ESF AF. The availability of pre-adapted cells saves significant time and expense associated with the adaptation of cultures. The Sf9 insect cell line is a clonal isolate derived from the parental Spodoptera frugiperda cell line IPLB-Sf-21-AE. Sf9 cells are adapted for suspension culture in ESF 921 or ESF AF and are available as a frozen vial or suspension culture. Sf9 cells are commonly used to produce recombinant baculoviral stocks and to produce recombinant proteins. • Recombinant protein expression from a variety of expression platforms • Good growth in adherent or suspension culture • Small, regular size that generates even monolayers and plaques Quality and performance testing To download Instructions for Use please visit: www.expressionsystems.com
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Expression Systems offers insect cell lines adapted to serum-free suspension culture in either ESF 921 or ESF AF. The availability of pre-adapted cells saves significant time and expense associated with the adaptation of cultures. The Sf21 insect cell line (IPLB-Sf-21-AE) is derived from ovarian cells of Spodoptera frugiperda. Sf21 cells are adapted for suspension culture in ESF 921 or ESF AF and are available as a frozen vial or suspension culture. • Recombinant protein expression from a variety of expression platforms • Good growth in adherent or suspension culture • Quality and performance testing To download Instructions for Use and/or COA please visit: www.expressionsystems.com
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Insect-XPRESS Medium is a protein-free formulation designed to support the growth of insect cell lines derived from Spodoptera frugiperda (Sf9 and Sf21) and Drosophila melanogaster (S2) Cell densities in excess of 8 3 10 6 cells/ml can be achieved with suspension cultures of Sf9 cells using Insect-XPRESS Medium and an excess of oxygen This formulation can also be used for stationary monolayer cultures and shake-flask cultures Insect-XPRESS Medium contains L-glutamine and supports superior production of recombinant proteins when using the Baculovirus Expression Vector System (BEVS) For cryopreservation of insect cells Insect-XPRESS can be mixed 50 50 with Cryoprotective Medium (Cat No 12-769E) All BioWhittaker Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures
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formulated for the growth of S2 cells from the fruit fly, Drosophila melanogaser. BioWhittaker™ Insect Cell Culture Media: Schneider's Drosophila Medium (Modified) may be used to culture a variety of other insect cell lines as well.