Cell Lines and Cell Cultures
ATCC Ligilactobacillus saerimneri
Whole-genome sequenced bacterial strain that was isolated from the stomach of a horse. This strain is cited to produce histidine decarboxylase.
ACROBiosystems HEK293 Human GIPR Stable Cell Line, High Expression, 2 Vials
HEK293/Human GIPR stable cell line engineered to express full-length human GIPR at high levels for robust cell-based assays and agonist screening. Surface expression is validated by flow cytometry. Supplied frozen with QC data and licensing support for commercial use.
- Supplied as frozen vials for long-term storage in liquid nitrogen.
- Each vial contains at least 5 x 10^6 cells in 1 mL serum-free cryopreservation medium.
- High receptor expression for a larger assay window and reproducible results.
- Validated for cell-based GIPR binding assays and cAMP accumulation agonist screening.
- Includes quality control data and documented passage stability.
Eurofins Discoverx PathHunter GPR120L Total Internalization HEK 293 Cell Line, 2 vials. (93-0801C1)
The PathHunter GPR120L Total Internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of GPR120L via detection of localization to the early endosome. The included cell line overexpresses EA-tagged GPR120L and a ProLink-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the GPR120L receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
iXCells Biotechnologies Human Hepatic Stellate Cells- Adult (HHSC)
iXCells Biotechnologies provides high quality Human Hepatic Stellate Cells (HHSC), which are isolated from adult human liver and cryopreserved with >1 million cells in each vial. Each vial is sufficient to seed on four 10 cm dishes, and cells will be ready in 5~6 days to be passaged at 1:3 ratio. HUSMC express Desmin and GFAP, and are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fung. HUSMC can further expand for 2-3 population doublings in¿Stellate Cell Growth Medium¿under the condition suggested by iXCells Biotechnologies.
Eurofins Discoverx PathHunter CHO-K1 GPR171 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0415C2A)
The PathHunter GPR171 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR171 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR171 and EA-tagged β-Arrestin-2. Activation of GPR171 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx Ready-to-Assay SST5 Somatostatin Receptor Frozen Cells, 2 Vials of Cells. (HTS139RTA)
The Ready-to-Assay SST5 Somatostatin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. somatostatin) based activation of SSTR5 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses SSTR5 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of SSTR5, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ATCC Influenza A virus (H3N2); A/Aichi/2/68; Cell culture adapted
Influenza A virus (H3N2) strain A/Aichi/2/68 is propagated in MDCK (NBL-2) cells (ATCC CCL-34). This strain was isolated in 1968 from a sailor on an Israeli ship docking in Aichi, Japan, and was deposited by the Centers for Disease Control and Prevention. It can be used in respiratory disease and influenza research.
Promocell GmbH PromoCell hMNC-CB Pellet from > 1 million Normal Human Mononuclear Cells, derived from Cord Blood, single donor
Cell pellet consisting of 1 million cells dissolved in 200µl RNAlater for subsequent RNA, DNA or protein analysis. Note: Cell pellets cannot be revived.Our Cell Pellets are stored in RNAlater – an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes our Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.Each Cell Pellet is made from 1 million cells and is dissolved in 200 µl RNAlater. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.
Eurofins Discoverx PathHunter U2OS KDR Activity Assay, 2 vials. (93-0903C3)
The PathHunter KDR RTK Activity cell line is a stable, engineered cell line for use in studying small molecule drug candidates targeting RTKs. This cell line enables assessment of RTK activation via detection of resulting KDR phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The cell line overexpresses ProLink-tagged fusion of the KDR kinase domain with transmembrane and extracellular ligand binding domain of EGFR; and also an EA-tagged SH2 domain protein. Upon ligand induced receptor activation, cross-phosphorylation of the receptor leads to SH2-EA recruitment and production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
ATCC MEF (DR4); Fibroblast; Mouse (Mus musculus)
For-profit Organizations: For every order of this item, you must work directly with the contributor, Whitehead Institute to (i) negotiate a research-use license, and/or (ii) have the contributor provide authorization to ATCC to ship this item under your existing license. We cannot ship this item until we receive communication directly from Whitehead Institute that we are authorized to ship each order. We are providing the following contact information, but this information may change without notice: Whitehead Institute for Biomedical Research Attn. Shoji Takahashi Phone: (617) 258-8930 Email: takahashi@wi.mit.edu. Once ATCC has received authorization from Whitehead Institute, your order will be reviewed, and this item will be released for shipment if all requirements are met. If you need assistance with your order, please contact our Customer Care team.
ATCC Staphylococcus; capitis; subsp. capitis
Staphylococcus capitis subsp. capitis strain LK 499 is a whole-genome sequenced bacterial type strain that was isolated from human skin.
STEMCELL Technologies Human Peripheral Blood B Cells, Frozen, Size: 2 x 10^7 cells
Human primary B cells derived from peripheral blood (PB) are isolated from mononuclear cells (MNCs) using the negative immunomagnetic selection technique. Cells are untouched and ready for downstream applications. PB was collected using acid-citrate-dextrose solution A (ACDA) as the anticoagulant.Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.Certain products are only available in select territories. Please contact your local Sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.Browse our Frequently Asked Questions (FAQs) (https://www.stemcell.com/primary-cells-faq) on Primary Cells.