General Cell Lines and Cell Cultures
Amsbio LLC Normal Human Normal Human Intr
Normal Human Normal Human Intrahepatic Biliary Epithelial Cells (Cholagioctyes); passage 1
Revvity Health Sciences Inc AequoZen Human Vasopressin V1B Cell Line, CHO-K1 Frozen Cells
AequoZen Human Vasopressin V1B Cell Line, CHO-K1 Frozen Cells
Revvity Health Sciences Inc cAMPZen Frozen Human Adenosine A2B Cell Line, HEK293 Cells
cAMPZen Frozen Human Adenosine A2B Cell Line, HEK293 Cells
Eurofins Discoverx PathHunter CHRM5 Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0657C3)
The PathHunter CHRM5 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of CHRM5 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CHRM5. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CHRM5-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Escherichia coli; Strain 2003-3014
Escherichia coli strain 2003-3014 is a whole-genome sequenced strain that was isolated from stool in Michigan. It is toxigenic and was confirmed by PCR to carry the stx1, stx2, and eae genes. This strain is serotype O26:H11 and it has applications for food testing, enteric disease research, and infectious disease research.
ATCC Malassezia globosa; CBS 7966
An ampoule containing viable cells (may include spores and mycelium) suspended in cryoprotectant.
Genscript Corporation CHO-K1/CD40 Stable Cell Line
Cluster of differentiation 40 CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects
ATCC Paraclostridium bifermentans; Strain 76
Paraclostridium bifermentans strain 76 is a bacterial anaerobe that is propagated at 37°C.
Eurofins Discoverx CHO-K1 F2RL1 Gq Cell Line, 2 vials. (95-1006C2)
The F2RL1 Gq cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serine proteases, SLIGRL-NH2) based activation of F2RL1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses F2RL1, a naturally Gq coupled GPCR. Upon activation of F2RL1, the resulting calcium activity can be fluorescently assayed using the Calcium No WashPLUS Detection Kit. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 AVPR2 β-Arrestin Cell Line, 2 vials. (93-0588C2)
The PathHunter AVPR2 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. vasopressin) based activation of AVPR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AVPR2 and EA-tagged β-Arrestin-2. Activation of AVPR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Amsbio LLC Luciferase (firefly) & CRE Expression cell line (RFP-Puro)
Luciferase (firefly) & CRE Expression cell line (RFP-Puro)
Eurofins Discoverx ChemiScreen CCR8 Chemokine Receptor Membrane Preparation, 1 vial. (HTS013M)
The ChemiScreen CCR8 Chemokine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CCR8 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach or to screen for GPCR activity using GTPγS assays. Increased binding affinities or GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen M1 Muscarinic Acetylcholine Receptor Membrane Preparation, 1 vial. (HTS044M)
The ChemiScreen M1 Muscarinic Acetylcholine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CHRM1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen LPA3 Lysophospholipid Receptor Stable Cell Line, 2 vials. (HTS130C)
The ChemiScreen LPA3 Lysophospholipid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. LPA) based activation of LPAR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses LPAR3 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of LPAR3, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.