General Cell Lines and Cell Cultures
Cell Applications Inc Proliferating Bovine Pulmonary Artery Endothelial Cells (BPAEC)
Proliferating Bovine Pulmonary Artery Endothelial Cells (BPAEC)
Cell Applications Inc Cryopreserved HiPSC, from Adult Female Dermal Fibroblasts
Cryopreserved HiPSC, from Adult Female Dermal Fibroblasts
Biochain Institute Inc Paraffin Tissue Section - Human Adult Normal: Liver, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Biochain Institute Inc Matched Pair - Paraffin Tissue Sections - Human Primary and Matched Metastatic Tumor (PM): Stomach, 5 x2 slides /PK
BioChain's human paraffin tissue (FFPE) section matched pair products include: Primary Pair (PP), Primary and Metastatic Pair (PM). PP consists of paraffin tissue sections of primary tumor and its adjacent normal tissue; PM consists of paraffin tissue sections of primary tumor and corresponding metastatic tumor. Paraffin tissue sections in each pair are prepared from the same donor. This product line is ideal for localization of tumor- specific genes and tumor metastatic genes.
Biochain Institute Inc Multiple Species Paraffin Tissue Array - Liver, 5 slides/PK
BioChain's tissue arrays or tissue microarrays (TMAs) are a useful tool in biomarker research and drug target validation. It consists of paraffin blocks that are assembled in array fashion to allow multiplex histological analysis. BioChain's TMAs provide a valuable tool for analysis of protein, RNA, or DNA molecules in oncology, both the malignant and metastatic markers. TMAs can also be constructed with tissues from other diseases and species.
Eurofins Discoverx PathHunter U2OS PDGFRb Functional Assay, 2 vials. (93-0493C3)
The PathHunter U2OS PDGFRb Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated PDGFRb dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged PDGFRb and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
ATCC Quantitative Synthetic; Human coronavirus; Strain NL63 RNA
Quantitative synthetic Human coronavirus NL63 RNA can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load. This preparation includes fragments from NSP3 (ORF 1A), Rdrp (nsp12), NTPase (nsp13), nsp16, spike protein, nucleocapsid, and 3’ untranslated regions.
Eurofins Discoverx PathHunter CHO-K1 OXTR β-Arrestin Cell Line, 2 vials. (93-0261C2)
The PathHunter OXTR cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. oxytocin) based activation of OXTR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OXTR and EA-tagged β-Arrestin-2. Activation of OXTR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Betacoronavirus 1 RNA; Strain: OC43; Human coronavirus OC43
Genomic RNA isolated from a preparation of cell lysate and supernatant from HCT-8 cells (ATCC CCL-244) infected with Betacoronavirus 1 strain OC43 (ATCC VR-1558).This product was prepared using methods known to inactivate viruses. It is suitable for use in RT-PCR or other RNA-based procedures. The source organism and host cells are also available through the ATCC catalog.
Eurofins Discoverx Ready-to-Assay GPR120 Receptor Frozen Cells, 2 Vials of Cells. (HTS225LRTA)
The Ready-to-Assay GPR120 Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. free fatty acids) based activation of FFAR4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FFAR4 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of FFAR4, the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
STEMCELL Technologies G-CSF and Plerixafor Mobilized Human Peripheral Blood Mononuclear Cells, Frozen, Size: 2.5 x 10^7 cells
Start your assays with ready-to-use, ethically sourced, mobilized primary mononuclear cells. We help you get the cells you need with personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications. Donors are mobilized with G-CSF, plerixafor, or G-CSF and plerixafor prior to collection. Isolated from leukapheresis samples using density gradient separation or red blood cell lysis and cryopreserved in CryoStorCS10 medium, cells are obtained using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. ACDA is added as an anticoagulant. Contact techsupport@stemcell.com for donor specifications (BMI category, smoking status, ethnicity, etc.). Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C. Certain products are only available in select territories.
Biochain Institute Inc Frozen Tissue Section - Human Adult Normal: Blood Vessel: Vein, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.