General Cell Lines and Cell Cultures
Genscript Corporation HEK293/GCGR/G15 Stable Cell L
Recombinant HEK293 cells stably overexpress human glucagon receptor (GCGR) on the surface and contain high levels of G protein G s to couple with the receptor in downstream signaling pathways
Eurofins Discoverx PathHunter U2OS FGFR1 Functional Assay, 2 vials. (93-1090C3)
The PathHunter U2OS FGFR1 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated FGFR1 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged FGFR1 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Cell Applications Inc Human Gastric Epithelial Cells Total Kit, adult
Human Gastric Epithelial Cells Total Kit, adult:(732Ga-05a) Cryopreserved (HGaEpC) 1 vial, adult(716DCMK-50) GI Epithelial Cell Defined Culture Media, 50ml(025-05) Coating Solution for GI Epithelial Cells(716T-20) Thawing Solution for GI Epithelial Cells
Biochain Institute Inc Paraffin Tissue Section - Human Prostate Tumor: Neoplasia of Prostate, 5 sides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. Each slide contains tissue from a single human tumor. Documentation of tissues' clinical histories may be available upon request. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Eurofins Discoverx ChemiScreen M2 Muscarinic Acetylcholine Receptor Membrane Preparation, 1 vial. (HTS115M)
The ChemiScreen M2 Muscarinic Acetylcholine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CHRM2 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Biochain Institute Inc Frozen Tissue Section - Dementia: Brain: Hippocampus, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
Abeomics Foxp3 Reporter Jurkat Line
The FOXP3 Leeporter Luciferase Reporter cell line is a stably transfected Jurkat T cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the Forkhead box P3 (FOXP3) promoter. As a member of the forkhead transcription factor family, FOXP3 is a key transcription factor that functions in the development and function of regulatory T cells. Functional activity of the cell line has been validated by phorbol 12-myristate 13-acetate (PMA) in the presence of ionomycin. Cell line application: (1) Monitor the FOXP3 induction activity, and (2) Screen for activators or inhibitors of FOXP3 indction. .
Eurofins Discoverx Ready-to-Assay α1B Adrenergic Receptor Frozen Cells, 2 Vials of Cells. (HTS158RTA)
The Ready-to-Assay α1B Adrenergic Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA1B GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADRA1B alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of ADRA1B, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen MC5 Melanocortin Receptor Membrane Preparation, 1 vial. (HTS155M)
The ChemiScreen MC5 Melanocortin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of MC5R surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx PathHunter CHO-K1 mEDNRA β-Arrestin Cell Line, 2 vials. (93-0685C2)
The PathHunter mEDNRA cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. endothelin-1) based activation of mEDNRA GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mEDNRA and EA-tagged β-Arrestin-2. Activation of mEDNRA stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Montana Molecular Human Protease-activated Receptor 2 (PAR2, FTRL1) BacMam, 30mL
Use this assay to express the human PAR2 receptor in your cells of interest. BacMam packaging provides efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in primary or iPSC-derived cells. Importantly, BacMam expression is easily titrateable to suit your experimental needs. This tool can be co-expressed with Montana Molecular's assays for DAG, Calcium, or arrestin to investigate signaling dynamics or receptor activation at the PAR2 receptor.
Amsbio LLC SRF-RE Leeporter™ Luciferase Reporter-HEK293 Cell Line
SRF-RE Leeporter™ Luciferase Reporter-HEK293 Cell Line
