General Cell Lines and Cell Cultures
Revvity Health Sciences Inc AequoZen Human Adrenergic α2B Cell Line, CHO-K1 Frozen Cells
AequoZen Human Adrenergic α2B Cell Line, CHO-K1 Frozen Cells
Eurofins Discoverx PathHunter Rat TrkA-P75 Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Rat TrkA-P75 Stable Cell Line Functional Kinase Assay (U2OS) measures TrkA-P75 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
ATCC Human adenovirus 1; Strain: Type 1
Human adenovirus 1 strain Type 1 is an animal virus propagated in HeLa cells (ATCC CCL-2). This strain was isolated from cat feces in Budapest, Hungary, in 1997.
ATCC pK18mosbsacB plasmid; in E. coli SCS110
pK18mobsacB is a cloning vector allowing mobilization into a wide range of Gram-negative and Gram-positive bacteria. It is distributed in Escherichia coli SCS110. After mobilization, the plasmid can be maintained by integration into the host chromosome via homologous recombination. Excision of the intervening plasmid sequence by a double cross-over event can be facilitated by the selection of medium containing 10% sucrose. The sacB gene has been modified to eliminate the HindIII and EcoRI sites in the coding region.
ATCC pRE112 plasmid; in E. coli
One of 4 allelic exchange suicide vectors (ATCC# 87691-87694) that provide both selection for chromosomal integration (ampR, cmLR, kanR or tetR) and counterselection for loss of vector DNA and the wild type allele.The construct is a suicide plasmid in any host not expressing pir. Cloned inserts may be integrated into the host chromosome (a single recombination event) following electroporation and appropriate antibiotic selection. - Gene 207: 149-157, 1998.The conditional R6K origin of replication requires that the pi protein be expressed in trans for plasmid maintenance.- Cell 15: 1199-1208, 1978Negative selection for sucrose sensitivity (sacB) selects for a second recombination event resulting in loss of vector DNA. The sacB1 allele is a variant of sacB with certain restriction sites removed by site directed mutagenesis. Expression of sacB confers sensitivity to sucrose. ? Mol. Microbiol. 18: 877-889, 1995.
Eurofins Discoverx ChemiScreen PK2 Prokineticin Receptor Membrane Preparation, 1 vial. (HTS182M)
The ChemiScreen PK2 Prokineticin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of PROKR2 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx PrecisION hGABAA α6/β3/γ2 Recombinant Stable Cell Line, 2 vials. (CYL3086)
The PrecisION hGABAA α6/β3/γ2 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying GABAA α6/β3/γ2 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses GABAA α6/β3/γ2 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Influenza A virus (H1N1); A/Virginia/ATCC3/2009; TC isolate
Influenza A virus (H1N1) strain A/Virginia/ATCC3/2009 is propagated in MDCK (NBL-2) cells (ATCC CCL-34). This strain was isolated in 2009 from a nasopharyngeal specimen from a patient positive for Influenza A in Virginia. It has applications in respiratory research and influenza research.
Eurofins Discoverx Ready-to-Assay M1 Acetylcholine (Muscarinic) Receptor Frozen Cells, 2 Vials of Cells. (HTS044RTA)
The Ready-to-Assay M1 Acetylcholine (Muscarinic) Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. acetylcholine) based activation of CHRM1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CHRM1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CHRM1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ATCC Quantitative Genomic; DNA from; Klebsiella oxytoca
ATCC Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.
Biochain Institute Inc Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Hippocampus, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. Each slide contains tissue from single human diseased sample. Documentation of tissues' clinical histories may be available upon request. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
ATCC Synthetic Middle East; respiratory syndrome; coronavirus RNA
Quantitative synthetic RNA for Middle East respiratory syndrome coronavirus can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load and for other molecular biology applications. This preparation includes fragments from the ORF1ab, ORF5, upper envelope (upE), ORF8b, nucleocapsid (N) protein gene, and 3' UTR regions.
Amsbio LLC C57BL/6 Mouse Brain (Coronal) slide (25-pack)
C57BL/6 Mouse Brain (Coronal) slide (25-pack)
Cell Applications Inc Cryopreserved Colonic Epithelial Cells (HCnEpC), adult
Cryopreserved Colonic Epithelial Cells (HCnEpC), adult