General Cell Lines and Cell Cultures
Promocell GmbH PromoCell HUF-c 500,000 Human Uterine Fibroblasts, cryopreserved
Primary Human Uterine Fibroblasts (HUF) are isolated from the uterine wall. During pregnancy, the uterine wall undergoes deep but reversible structural and biochemical changes. The uterine wall is the target of hormones and growth factors that act simultaneously and lead to extensive modifications of the extracellular matrix. This and other morphological and biochemical effects can be studied in vitro using HUF. Available formats:* Cryopreserved: Cryogenic vial containing 500.000 viable cells.
ATCC Pfizer Pneumococcal polysaccharide powder Type 70 (d33F) 200mg
Purified pneumococcal polysaccharide type 33F is manufactured by Pfizer, Inc. for small quantity distribution by ATCC. The pneumococcal polysaccharide type 33F can be used to assay for type-specific antibodies, research immune responses, and study immunodeficiencies.
ATCC Pfizer Pneumococcal polysaccharide powder Type 54 (d15B) 2mg
Purified pneumococcal polysaccharide type 15B is manufactured by Pfizer, Inc. for small quantity distribution by ATCC. The pneumococcal polysaccharide type 15B can be used to assay for type-specific antibodies, research immune responses, and study immunodeficiencies.
Eurofins Discoverx Ready-to-Assay 5-HT2C Serotonin Receptor Frozen Cells, 2 Vials of Cells. (HTS132RTA)
The Ready-to-Assay 5-HT2C Serotonin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. serotonin/5-HT) based activation of HTR2C GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses HTR2C alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of HTR2C, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Promocell GmbH PromoCell HPF-c 500,000 Human Pulmonary Fibroblasts, cryopreserved
Primary Human Pulmonary Fibroblasts (HPF) are isolated from human lung tissue. They are often used for studying lung tissue repair, tissue remodeling after injury, or the response after pulmonary inflammation. Fibroblast disorders like pulmonary fibrosis or hypoxia-induced pulmonary hypertension can also be investigated with cultured HPF.
Eurofins Discoverx ChemiScreen CB1 Cannabinoid Receptor Membrane Preparation, 1 vial. (HTS019M)
The ChemiScreen CB1 Cannabinoid Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CNR1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach or to screen for GPCR activity using GTPγS assays. Increased binding affinities or GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen Rhesus Macaque CCR5 Chemokine Receptor Membrane Preparation, 1 vial. (HTS010M)
The ChemiScreen Rhesus Macaque CCR5 Chemokine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CCR5 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen MCH1 Melanin-concentrating Hormone Receptor Stable Cell Line, 2 vials. (HTS025C)
The ChemiScreen MCH1 Melanin-concentrating Hormone Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. MCH) based activation of MCHR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MCHR1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of MCHR1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
ATCC Aspergillus brasiliensis; WLRI 034 (120); genomic DNA
Genomic DNA isolated from Aspergillus brasiliensis WLRI 034(120) (ATCC 16404). This whole-genome sequenced product can be used in PCR and other molecular biology applications.