General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter CHO-K1 THRβ Protein Interaction Cell Line, 2 vials. (93-0391C2)
The PathHunter THRβ Protein Interaction cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting NHR activation and recruitment of cofactors. This cell line enables assessment of ligand (e.g. triiodothyronine) based NHR activation via detection of SRCP (steroid receptor co-activator peptide) recruitment. The included cell line overexpresses ProLink-tagged THRβ NHR and EA-tagged steroid receptor co-activator peptide (SRCP). Upon activation of THRβ, induced interaction with SRCP leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/nhr.
Eurofins Discoverx PathHunter CHO-K1 NPR1 Functional Assay, 2 vials. (93-0804C2)
The PathHunter CHO-K1 NPR1 Functional Assay is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting NPR1. This cell line enables assessment of ligand (e.g. ANP) based stimulation of receptor activity via detection of resulting increases in the levels of intracellular cGMP. This product uses EFC technology. The included cell line overexpresses untagged NPR1 Upon activation of the pathway, the increase in levels of intracellular cGMP leads to production of increased EFC signal when the cGMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
ATCC Klebsiella pneumoniae; Strain 1002565
Klebsiella pneumoniae subsp. pneumoniae strain 1002565 is a whole-genome sequenced bacterium that was isolated from a respiratory sample. This strain is resistant to ertapenem and imipenem and was confirmed by PCR to contain the blaNDM gene. This bacterial culture can be used in antimicrobial resistance research, drug development, infectious disease research, and respiratory disease research.
Eurofins Discoverx KILR SKOV3 Cell Pool, 2 vials. (97-1007P023)
The KILR SKOV3 cell pool is a stable, engineered target cell pool, which closely reflects the heterogeneous native protein expression of tumor environments, used to measure direct cell death mediated by immunooncology treatments (e.g. checkpoint modulators, bispecifics, CAR-T, etc.) via Antibody Dependent Cell-mediated Cytotoxicity (ADCC), Complement Dependent Cytotoxicity (CDC), and Antibody Dependent Cell Phagocytosis (ADCP). This product uses EFC technology. The cell pool is a heterogeneous population of SKOV3 cells that overexpress a ProLabel-tagged housekeeping gene. Upon effector cells-mediated cell lysis, ProLabel is released to the media, where EA in the detection kit produces an EFC signal proportional to the level of ProLabel released. This allows for calculation of percent lysis of the KILR cell pool. This stable cell pool is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kilr.
Genscript Corporation CHO-K1/ADRA2B/Gqi5 Stable Cell
Adrenoceptors are 7-transmembrane receptors which mediate the central and peripheral actions of the neurotransmitter noradrenaline (norepinephrine) and the hormone and neurotransmitter adrenaline (epinephrine) Based on both pharmacological and molecular evidence they are divided into three major types - 1 2 and The 2 adrenoceptors include 3 subtypes - 2A 2B and 2C - each of which couples to G sub i/o /sub protein primarily br The 2B adrenoceptors (ADRA2B) is expressed in the kidney liver brain lung heart and skeletal muscle The development of 2B and 2C knock-out mice has shown that these two subtypes are not involved in the central hypotensive response to 2 agonists
Eurofins Discoverx PrecisION hASIC3 Recombinant Stable Cell Line, 2 vials. (CYL3055)
The PrecisION hASIC3 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying ASIC3 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses ASIC3 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Discovery Life Sciences Ulcerative Colitis PBMC
PBMCs Peripheral Blood Mononuclear Cells are immune cells isolated from whole blood and viably cryopreserved providing a foundational material for immunology studies cell therapy research and functional immune assays
Amsbio LLC Human Cervical cancer and normal adjacent tissue FFPE block
Human Cervical cancer and normal adjacent tissue FFPE block
Eurofins Discoverx PathHunter U2OS PTGFR β-Arrestin Cell Line, 2 vials. (93-0936C3)
The PathHunter PTGFR cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. cloprostenol) based activation of PTGFR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGFR and EA-tagged β-Arrestin-2. Activation of PTGFR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Genscript Corporation CHO-K1/D1 Stable Cell Line
Dopamine is the predominant catecholamine neurotransmitter found in mammalian brain where it controls a variety of functions including locomotor activity cognition emotion positive reinforcement food intake and endocrine regulation It also plays multiple roles in the periphery as a modulator of cardiovascular function catecholamine release hormone secretion vascular tone renal function and gastrointestinal motility The dopamine receptor family consists of five members which are classified into two groups D1-like (D1 and D5) and D2-like (D2 D3 and D4)
Eurofins Discoverx Ready-to-Assay EP1 Prostanoid Receptor Frozen Cells, 2 Vials of Cells. (HTS099LRTA)
The Ready-to-Assay EP1 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin E2) based activation of PTGER1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER1, the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Lonza Walkersville HIAEC – Human Iliac Artery Endothelial Cells
Primary Human Iliac Artery (HIAEC) Endothelial Cells are isolated from normal tissue. Additional donor characteristics including e.g. heart disease, hypertension, or smoking history may be available. For further information please contact our Scientific Support Team. Clonetics Cells, Medium and Reagents are quality tested together and guaranteed to give optimum performance as a complete cell system. Cryopreserved HIAEC are shipped in third passage.Cryopreserved HIAEC are guaranteed through 10 population doublings. HIAEC express CD31/105, von Williebrand Factor VIII, and are positive for acetylated low density lipoprotein uptake. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.Content:1 x Cryopreserved Ampule with ≥500,000 cells/vial
ATCC Neisseria gonorrhoeae; genomic DNA
Genomic DNA isolated from Neisseria gonorrhoeae strain [ATCC 31426]. This bacterial strain is also available as ATCC Catalog No.: 53422™.