General Cell Lines and Cell Cultures
ATCC Oral Microbiome; Genomic Mix
NGS Standards are mock microbial communities that mimic mixed metagenomic samples. This product comprises an even mixture of genomic DNA prepared from fully sequenced, characterized, and authenticated bacterial species observed in normal and atypical oral microbial communities. Each order for this product is provided with access to run 20 analyses on One Codex, the leading bioinformatics platform for microbial genomics and metagenomics. Specification Range: 1.2x108 genome copies/vial ± 1 log* *Genomic DNA concentrations indicate ATCC manufacturing specifications and are provided as a reference only
ATCC SIMA9 BRAIN MICROGLIA MOUSE MU
SIM-A9 is a microglial cell line that was derived from cerebral cortex tissues collected and pooled from mouse pups. The tissues were dissociated by trypsinization and plated. On day 14 the microglial cells were harvested by vigorously shaking the flasks and the plating out detached microglial cells. The plates were maintained for 2 weeks at which time an unexpected, extensive proliferation of microglial cells was observed. The cells were passaged 7 times over a 4-week period and were determined to have become spontaneously immortalized. The cells were then plated out in a limited dilution procedure and colonies derived from wells observed to contain a single cell were expanded. A clone designated as “A-9” gave rise to this cell line. These spontaneously immortalized micgoglial cells exhibit phagocytic activity upon liposaccharide stimulation and TNFα cytokine secretion upon β-amyloid exposure. These cells may be used in place of primary microglial cultures, whic
Eurofins Discoverx Ready-to-Assay APJ Apelin Receptor Frozen Cells, 2 Vials of Cells. (HTS068RTA)
The Ready-to-Assay APJ Apelin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. apelin) based activation of AGTRL1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses AGTRL1 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of AGTRL1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ATCC Gardnerella vaginalis; 594
Gardnerella vaginalis strain 594 [NCTC 10287] is a whole genome-sequenced strain that was isolated from vaginal secretions. This bacterial type strain has applications in bioinformatics.
ATCC HEK001; Skin Keratinocyte; Human (Homo sapiens)
HEK001 is a keratinocyte that was isolated from the scalp of a 65-year-old, male patient. The cells were transfected with plasmid p1321 which contains human papillomavirus (HPV) 16 E6 and E7 genes. HEK001 can be used in toxicology research.
ATCC Ruminococcus bromii; Strain VPI 6883
Ruminococcus bromii strain VPI 6883 is a whole-genome sequenced bacterial type strain that was isolated from human feces.
ATCC MCF7-Luc2; Breast Adenocarcinoma; Human (Homo sapiens)
For-profit organizations: For every order of this item, you must provide a signed Material Transfer Agreement Addendum for Screening Application. We cannot ship this item until we receive this addendum. The person signing the addendum as the principal investigator must match the end user as listed on the applicable sales order for the item.Email the signed addendum to SalesPermits@atcc.org with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. Additional fees may apply if this product is being used for a screening use (ATCC ACS-2103F), and these fees will be applied after your order is confirmed. If you need assistance with your order, please contact our Customer Care team.
ATCC Escherichia coli; Quantitative DNA
Quantitative Genomic DNA from Escherichia coli strain 2003-3014 can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.
ATCC Legionella gormanii
Legionella gormanii strain LS-13 [ALLO3] is a whole-genome sequenced bacterial type strain that was isolated from soil.
ATCC Epidermophyton floccosum
An ampoule containing viable cells (yeast cells, spores, or agar cubes with mycelia) suspended in cryoprotectant.
