General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter CHO-K1 mCCR6 β-Arrestin Cell Line, 2 vials. (93-0694C2)
The PathHunter mCCR6 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CCL20/MIP-3α) based activation of mCCR6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR6 and EA-tagged β-Arrestin-2. Activation of mCCR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx Ready-to-Assay CCR5 Chemokine Receptor Frozen Cells, 2 Vials of Cells. (HTS010RTA)
The Ready-to-Assay CCR5 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. MIP, RANTES) based activation of CCR5 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CCR5 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CCR5, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ACROBiosystems CHO/Human CD16a (158V) Stable Cell Line (Medium Expression) Development Service
CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages.CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibodydependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in su
Eurofins Discoverx PrecisION hKv1.3 Recombinant Stable Cell Line, 2 vials. (CYL3016)
The PrecisION hKv1.3 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying Kv1.3 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses Kv1.3 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Eurofins Discoverx ChemiScreen D2 Dopamine Receptor Stable Cell Line, 2 vials. (HTS039C)
The ChemiScreen D2 Dopamine Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. dopamine) based activation of DRD2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses DRD2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of DRD2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx PathHunter HEK 293 IGF1R Functional Assay, 2 vials. (93-0505C1)
The PathHunter HEK 293 IGF1R Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated IGF1R dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged IGF1R and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx mGlu5 (GRM5) Gq Calcium Signaling Cell-Based Assay Kit (U2OS)
The mGlu5 (GRM5) Gq Stable Cell Line Calcium Signaling Assay (U2OS) contains a stable clonal cell line used to measure GRM5 (GPCR) activation via an increase in intracellular calcium. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter CHO-K1 CALCR-RAMP2 (AMY2) β-Arrestin Cell Line, 2 vials. (93-0267C2)
The PathHunter CALCR-RAMP2 (AMY2) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. calcitonin) based activation of CALCR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CALCR and EA-tagged β-Arrestin-2. Activation of CALCR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC MCF7-Luc2; Breast Adenocarcinoma; Human (Homo sapiens)
For-profit organizations: For every order of this item, you must provide a signed Material Transfer Agreement Addendum for Screening Application. We cannot ship this item until we receive this addendum. The person signing the addendum as the principal investigator must match the end user as listed on the applicable sales order for the item.Email the signed addendum to SalesPermits@atcc.org with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. Additional fees may apply if this product is being used for a screening use (ATCC ACS-2103F), and these fees will be applied after your order is confirmed. If you need assistance with your order, please contact our Customer Care team.
ATCC Escherichia coli; Quantitative DNA
Quantitative Genomic DNA from Escherichia coli strain 2003-3014 can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.
Lonza Walkersville SAEC - for S-ALI Medium
Lonza s Human Small Airway Epithelial Cells (SAEC) are isolated from normal human lung tissue the distal portion of the lung in the 1mm bronchiole area Contact our Scientific Support team for additional details Cryopreserved Clonetics SAECs are guaranteed through 15 population doublings and stain positive for cytokeratin 19 SAECs labeled CC-2547S are suitable for air-liquid interface studies and are guaranteed for differentiation marked by cilia formation mucin production andTEER levels All cells test negative for mycoplasma bacteria yeast and fungi HIV-1 hepatitis B andhepatitis C are not detected for all donors and/or cell lots A Certificate of Analysis is provided for each cell lot purchased
Biochain Institute Inc Paraffin Tissue Section - Human Adult Normal: Heart: Auricula (right), 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Biochain Institute Inc Paraffin Tissue Section - Human Colon Tumor: Diffuse Type Non-Hodgkin's Lymphoma, 5 sides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. Each slide contains tissue from a single human tumor. Documentation of tissues' clinical histories may be available upon request. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Eurofins Discoverx cAMP Hunter CHO-K1 CALCRL-RAMP1 Gs Cell Line, 2 vials. (95-0164C2)
The cAMP Hunter CALCRL-RAMP1 Gs cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. β-CGRP) based activation of CALCRL GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CALCRL, a naturally Gs coupled GPCR, alongside modulator protein RAMP1. Upon activation of CALCRL, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. This product expresses CALCRL alongside the receptor modifying protein RAMP1, which increases activity of the CALCRL receptor For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 MRGPRX4 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0541C2A)
The PathHunter MRGPRX4 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of MRGPRX4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MRGPRX4 and EA-tagged β-Arrestin-2. Activation of MRGPRX4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.