General Cell Lines and Cell Cultures
Eurofins Discoverx cAMP Hunter CHO-K1 HTR1B Gi cell Line, 2 vials. (95-0162C2)
The cAMP Hunter HTR1B Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin/5-HT) based activation of HTR1B GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type HTR1B, a naturally Gi/Go coupled GPCR. Upon activation of HTR1B, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx cAMP Hunter CHO-K1 CCR10 Gi Cell Line, 2 vials. (95-0175C2)
The cAMP Hunter CCR10 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CCL27) based activation of CCR10 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CCR10, a naturally Gi/Go coupled GPCR. Upon activation of CCR10, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biochain Institute Inc Frozen Tissue Array - Rabbit Male Adult Normal Tissue, Multi-tissue I, 5 slides/PK
BioChain's rabbit normal multiple frozen tissue arrays are designed for studying tissue specific cellular localization of genes or proteins of interest in different rabbit tissues. Up to 30 different frozen tissues from the same male or female rabbit are mounted on positively charged glass slides. Total RNA and cDNA from those tissues may also be available upon request.
Lonza Walkersville Hu Chondrocyte Nufe Kit
The Nucleofector 2b Device (or older generations I or II) works cell type specific kits each of them dedicated to an individual primary cell This Nucleofector Kit is the optimal kit for efficient transfection of primary human chondrocytes The respective cell type-specific Optimized Protocol or recommendation is available under Instructions or in our Lonza Bioscience knowledge database In this Optimized Protocol the best Nucleofection conditions are indicated In addition we share our experience and knowledge for treatment of this cell type
Eurofins Discoverx Ready-to-Assay NK3 Tachykinin Receptor Frozen Cells, 2 Vials of Cells. (HTS190RTA)
The Ready-to-Assay NK3 Tachykinin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. tachykinins) based activation of TACR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses TACR3 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of TACR3, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ATCC AsPC-1; Pancreatic Cancer; Human (Homo sapiens)
AsPC-1 is cell line derived from nude mouse xenografts initiated with cells from the ascites of a 62-year-old, White, female patient with cancer of the pancreas. This cell line can be used in cancer research.
Charles River Labs Cell Solutions PB DENDRITIC CELLS CRYO 2M
For Research Use Only. Human Normal Peripheral Blood Dendritic Cells - Cryopreserved, 2 million cells/vial. Derived from purified CD14 monocytes.CD14 monocytes are isolated from PBMCs and are cultured in the presence of GM-CSF and IL-4 for 7 days.
ATCC Trypsin/EDTA, 1X, 100 ML
The Trypsin-EDTA Solution is 0.25% Trypsin/0.53 mM EDTA in Hanks Balanced Salt Solution without calcium or magnesium. For dissociation of cell monolayers. Trypsin-EDTA solution is suitable for most but not for all adherent cell lines. This product has applications for cell culture, cell growth, and viability. For cell line-specific information, please go to the appropriate product page on the web, refer to the product sheet supplied with the cell line, or contact ATCC Technical Service.
Electron Microscopy Sciences Rodent Brain Matrix Adult Rat, 200-400g, Coronal
RBM allow the investigator to slice either coronal (perpendicular to center line) or sagittal (parallel to center line) sections through the brain at intervals as small as 1mm. All RBM's are identical and durable to insure reproducible sections in the day-to-day, year-to-year, or on a between laboratory basis.
Constructed from high grade zinc to allow for rapid temperature equilibration, easy cleaning, and sterilization. The matrices are designed to accommodate the brains of rodents placed ventral side up. The channels are precisely cut at 1 mm intervals and 0.3 mm wide.
Size: 200-400g
Type: Coronal
Genscript Corporation CHO-K1/G15/AMY1 Stable Cell L
Recombinant CHO-K1 cells stably overexpress human receptor activity modifying protein 1 (RAMP1) and calcitonin receptor (CTR) on the surface Amylin receptors (AMYR) are a class of special protein heterodimers composed of a CTR combined with one of three homologous receptor activity-modifying proteins (RAMP1 RAMP2 RAMP3) forming three subtypes (AMY1 AMY2 AMY3) This cell contain high levels of G protein G s to couple with the receptor in downstream signaling pathways
Measles virus strain Edmonston (ATCC VR-24™)
Measles virus strain Edmonston (ATCC VR-24™) is a whole-genome sequenced prototype strain isolated in 1954 from a human patient in Massachusetts. Propagated in MRC-5 cells, it is used in respiratory and infectious disease research.
Recommended companion products:
MRC-5 cells (Fisher Cat. No. 50-238-3231, ATCC CCL-171™)
EMEM (Fisher Cat. No. 50-238-2632, ATCC 30-2003™)
Fetal bovine serum (Fisher Cat. No. 50-238-3771, ATCC 30-2020™).
Cayman Chemical Glucagon RECPTR ntagonIst
A competitive antagonist of GCGR (IC50 = 181 nM); blocks glucagon-induced glycogenolysis in primary human hepatocytes, isolated liver, and humanized mice
STEMCELL Technologies Human Peripheral Blood Gamma Delta T Cells, Frozen
Gamma delta T cells (TCRγδ+CD3+) are isolated from peripheral blood using negative immunomagnetic separation and cryopreserved in animal component-free CryoStor®CS10 medium (Catalog #07930). Cells are obtained using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation, high-resolution HLA typing (Class I and Class II alleles), and viral screening status are available upon request. Acid-citrate-dextrose solution A (ACDA) is added during collection as an anticoagulant.
Cayman Chemical al-trns RetInal 100mg
A natural retinoid that associates with CRBP-I and CRBP-II (Kds = 50 and 90 nM, respectively), which are involved in the intracellular transport of retinol; an intermediate of the phototransduction pathway of photoreceptors; can form toxic conjugates with proteins and lipids, leading to degeneration of the retina
ATCC Genomic RNA from; SARS-CoV-2; variant B.1.1.7
Genomic RNA isolated from a preparation of Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) isolate USA/CA_CDC_5574/2020. This product can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.