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Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies (44,275)
Mouse Secondary Antibodies (14,507)
Rabbit Secondary Antibodies (6,327)
Donkey Secondary Antibodies (4,321)
Rat Secondary Antibodies (1,240)
Sheep Secondary Antibodies (703)
Chicken Secondary Antibodies (403)
Bovine Secondary Antibodies (257)
Horse Secondary Antibodies (76)
Pig Secondary Antibodies (76)
Alpaca Secondary Antibodies (62)
Hamster Secondary Antibodies (55)
Biosimilar Secondary Antibodies (9)
Human Secondary Antibodies (7)
Duck Secondary Antibodies (3)

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1,486 – 1,500 of 72,126 results
1,486

Donkey anti-Rat IgG (H+L) Secondary Antibody, PE, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rat
Host Species Donkey
Conjugate PE
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Formulation PBS
Classification Polyclonal
Immunogen Rat IgG whole molecule
Primary or Secondary Secondary
1,487

Donkey anti-Mouse IgG (H+L) Secondary Antibody, DyLight 594 (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Mouse
Host Species Donkey
Conjugate DyLight 594
Applications Flow Cytometry,Immunomicroscopy
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Flow Cytometry 1:20 - 1:2000, Immunomicroscopy 1:20 - 1:2000
Classification Polyclonal
Immunogen Purified Mouse IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on mouse IgG and light chains on all mouse immunoglobulins. This antibody has been pre-adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat and sheep IgG.
1,488

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Rhodamine, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Species Rabbit
Host Species Donkey
Conjugate Rhodamine
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Dilution Western Blot 1:1000 - 1:10000, Flow Cytometry 1:500 - 1:2500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Classification Polyclonal
Immunogen Rabbit IgG whole molecule
Primary or Secondary Secondary
Test Specificity Prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Fluorescein, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.
1,489

Invitrogen™ Donkey anti-Sheep IgG (H+L) Secondary Antibody, TRITC

Donkey Polyclonal Secondary Antibody

ENCOMPASS_PREFERRED
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Target Species Sheep
Host Species Donkey
Conjugate TRITC
Applications Immunocytochemistry
Form Lyophilized
Isotype IgG
Concentration 1 mg/mL
Antigen Sheep IgG (H+L)
Regulatory Status RUO
Purification Method Affinity chromatography
Product Type Secondary Antibody
Formulation PBS with 1% BSA and 0.05% sodium azide; pH 7.2
Immunogen Gamma Immunoglobins Heavy and Light chains.
Classification Polyclonal
Primary or Secondary Secondary
1,490

Donkey anti-Human IgG (H+L) Secondary Antibody [Janelia Fluor 669], Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Human
Host Species Donkey
Conjugate Janelia Fluor 669
Applications Western Blot,ELISA,Immunohistochemistry
Form Purified
Isotype IgG
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Human IgG whole molecule
Primary or Secondary Secondary
1,491

Donkey anti-Goat IgG (H+L) Secondary Antibody, DyLight 680, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Goat
Host Species Donkey
Conjugate DyLight 680
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Frozen),Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against goat IgG.
Primary or Secondary Secondary
1,492

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, FITC, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rabbit
Host Species Donkey
Conjugate FITC
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation PBS
Classification Polyclonal
Immunogen This Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against rabbit IgG.
Primary or Secondary Secondary
1,494

Donkey anti-Mouse IgG (H+L) Secondary Antibody, PE, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Mouse
Host Species Donkey
Conjugate PE
Applications ELISA,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation PBS
Classification Polyclonal
Immunogen Mouse IgG whole molecule
Primary or Secondary Secondary
1,495

Donkey anti-Rat IgG (H+L) Secondary Antibody, Alkaline Phosphatase (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Rat
Host Species Donkey
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1 mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:500 - 1:2500, ELISA 1:2000 - 1:10000, Immunohistochemistry 1:200 - 1:1000
Classification Polyclonal
Immunogen Rat IgG whole molecule
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, and Sheep Serum Proteins. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
1,496

Donkey anti-Rabbit IgG (H+L) Secondary Antibody [Janelia Fluor 669], Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rabbit
Host Species Donkey
Conjugate Janelia Fluor 669
Applications Western Blot,ELISA,Immunohistochemistry,Immunocytochemistry/Immunofluorescence,Immunohistochemistry (Paraffin)
Form Purified
Isotype IgG
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Donkey anti-Rabbit IgG (H+L) Secondary Antibody was developed against rabbit IgG-heavy and light chain.
Primary or Secondary Secondary
1,497

Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody has been used in 2 publications

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Mouse
Host Species Donkey
Conjugate Unconjugated
Applications ELISA,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000, Immunocytochemistry/Immunofluorescence, Fluorophore-linked immunosorbent assay
Classification Polyclonal
Immunogen Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) was produced by repeated immunization with Mouse IgG whole molecule in donkey.
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat, and Sheep Serum Proteins. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. Specificity was confirmed using ELISA at less than 1.0% cross reactivity against IgG proteins purified from the species listed above.
1,498

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, FITC (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Rabbit
Host Species Donkey
Conjugate FITC
Applications Flow Cytometry,Immunomicroscopy
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Flow Cytometry 1:20-1:2000, Immunomicroscopy 1:20-1:2000
Classification Polyclonal
Immunogen Purified Rabbit IgG (H&L)
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on rabbit IgG and light chains on all rabbit immunoglobulins. This antibody has been pre-adsorbed against mouse IgG and serum proteins.
1,499

Donkey anti-Rat IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Rat
Host Species Donkey
Conjugate HRP
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:1000 - 1:10000, ELISA 1:10000 - 1:50000, Immunohistochemistry 1:500 - 1:2500
Classification Polyclonal
Immunogen Rat IgG whole molecule
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, and Sheep Serum Proteins. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
1,500

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Allophycocyanin, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rabbit
Host Species Donkey
Conjugate APC
Applications ELISA,Flow Cytometry,Immunoassay,Immunocytochemistry/Immunofluorescence,Immunodiffusion,Immunohistochemistry,Immunohistochemistry (Frozen),Immunoprecipitation,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Formulation PBS
Classification Polyclonal
Immunogen This Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against purified rabbit IgG (H&L).
Primary or Secondary Secondary