Secondary Antibodies
Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies
(44,204)
Mouse Secondary Antibodies
(14,593)
Rabbit Secondary Antibodies
(6,310)
Donkey Secondary Antibodies
(4,319)
Rat Secondary Antibodies
(1,238)
Sheep Secondary Antibodies
(701)
Chicken Secondary Antibodies
(403)
Bovine Secondary Antibodies
(257)
Pig Secondary Antibodies
(77)
Horse Secondary Antibodies
(74)
Alpaca Secondary Antibodies
(62)
Hamster Secondary Antibodies
(57)
Biosimilar Secondary Antibodies
(9)
Human Secondary Antibodies
(7)
Duck Secondary Antibodies
(3)
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Goat anti-Rabbit IgG Fc Secondary Antibody, FITC, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Rabbit |
| Host Species | Goat |
| Conjugate | FITC |
| Applications | Flow Cytometry,Immunomicroscopy |
| Isotype | IgG |
| Concentration | 1.0mg/mL |
| Antigen | IgG Fc |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Flow Cytometry 1:20-1:2000, Immunomicroscopy 1:20-1:2000 |
| Classification | Polyclonal |
| Immunogen | Purified Rabbit IgG, Fc fragment |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with heavy gamma chains on rabbit IgG |
Donkey anti-Goat IgG (H+L) Secondary Antibody, DyLight 488 (Pre-adsorbed), Novus Biologicals™
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Donkey Polyclonal Antibody has been used in 2 publications
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Goat |
| Host Species | Donkey |
| Conjugate | DyLight 488 |
| Applications | Dot Blot,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Frozen),Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | LYOPH |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:10000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:5000, Immunohistochemistry-Frozen, Dot Blot, Fluorophore-linked immunosorbent assay 1:20000 |
| Classification | Polyclonal |
| Immunogen | Goat IgG whole molecule |
| Reconstitution | Reconstitute with 100 ul deionized water (or equivalent). |
| Primary or Secondary | Secondary |
| Test Specificity | This antibody was pre-adsorbed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit, and Rat Serum Proteins. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins. This antibody will react with heavy chains of Goat IgG and with light chains of most Goat immunoglobulins. |
Goat anti-Mouse IgG (H+L) Secondary Antibody, Alkaline Phosphatase (Pre-adsorbed), Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Target Species | Mouse |
| Host Species | Goat |
| Conjugate | Alkaline Phosphatase (AP) |
| Applications | ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | 1.50mg/mL |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin |
| Classification | Polyclonal |
| Immunogen | Purified Mouse IgG (H&L) |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with heavy gamma chains on mouse IgG and light chains on all mouse immunoglobulins. This antibody has been pre-adsorbed against bovine, goat, human, rabbit, and rat IgG. |
Goat anti-Human IgE Heavy Chain Secondary Antibody (Pre-adsorbed), Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Human |
| Host Species | Goat |
| Conjugate | Unconjugated |
| Applications | ELISA,Immunoassay,Immunodiffusion,Immunohistochemistry,Immunoprecipitation,Western Blot |
| Isotype | IgE |
| Concentration | 4.5mg/mL |
| Antigen | IgE Heavy Chain |
| Gene Symbols | IGHE |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Immunoassay |
| Gene Alias | Constant Region of Heavy Chain of IgE, Ig Epsilon Chain C Region, Ig Epsilon Chain C Region ND, IgE, Immunoglobulin Epsilon, immunoglobulin heavy constant epsilon |
| Gene ID (Entrez) | 3497 |
| Classification | Polyclonal |
| Immunogen | Purified Human IgE |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with heavy epsilon chains on human IgE. This antibody has been pre-adsorbed against bovine, mouse, and rabbit serum proteins. |
Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage. |
|---|---|
| Target Species | Rabbit |
| Host Species | Goat |
| Conjugate | HRP |
| Applications | ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | LYOPH |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin |
| Classification | Polyclonal |
| Immunogen | Purified Rabbit IgG (H&L) |
| Reconstitution | Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with heavy gamma chains on rabbit IgG and light chains on all rabbit immunoglobulins. This antibody has been pre-adsorbed against human, mouse and rat serum. |
Goat anti-Guinea Pig IgG (H+L) Secondary Antibody, DyLight 550, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage. |
|---|---|
| Target Species | Guinea Pig |
| Host Species | Goat |
| Conjugate | DyLight 550 |
| Applications | Flow Cytometry,Immunomicroscopy |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | LYOPH |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Flow Cytometry 1:20 - 1:2000, Immunomicroscopy 1:20 - 1:2000 |
| Classification | Polyclonal |
| Immunogen | Purified Guinea Pig IgG (H&L) |
| Reconstitution | Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with heavy gamma chains on guinea pig IgG and light chains on all guinea pig immunoglobulins |
Goat anti-Chicken IgY (H+L) Secondary Antibody, DyLight 350, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage. |
|---|---|
| Target Species | Chicken |
| Host Species | Goat |
| Conjugate | DyLight 350 |
| Applications | Flow Cytometry,Immunomicroscopy |
| Isotype | IgG |
| Concentration | LYOPH |
| Antigen | IgY (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Flow Cytometry 1:20 - 1:2000, Immunomicroscopy 1:20 - 1:2000 |
| Classification | Polyclonal |
| Immunogen | Purified Chicken IgG/Y (H&L) |
| Reconstitution | Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with heavy gamma chains on chicken IgG/Y and light chains on all chicken immunoglobulins |
Goat anti-Rat IgG (H+L) Secondary Antibody (Pre-adsorbed), Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Target Species | Rat |
| Host Species | Goat |
| Conjugate | Unconjugated |
| Applications | Chemiluminescence Immunoassay (CIA),ELISA,Flow Cytometry,Immunoassay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunomicroscopy,Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | 4.5mg/mL |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Immunoassay |
| Classification | Polyclonal |
| Immunogen | Purified Rat IgG (H&L) |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with heavy gamma chains on rat IgG and light chains on all rat immunoglobulins. This antibody has been pre-adsorbed against human and mouse lgG (highly absorbed against mouse IgG). |
Sheep anti-Rabbit IgG (H+L) Secondary Antibody, Alkaline Phosphatase (Pre-adsorbed), Novus Biologicals™
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Sheep Polyclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Rabbit |
| Host Species | Sheep |
| Conjugate | Alkaline Phosphatase (AP) |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | 1 mg/mL |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:500 - 1:2500, ELISA 1:2000 - 1:10000, Immunohistochemistry 1:200 - 1:1000 |
| Classification | Polyclonal |
| Immunogen | Rabbit IgG whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | This antibody was pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat, and Sheep Serum Proteins. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat and Sheep Serum Proteins. |
Donkey anti-Sheep IgG (H+L) Secondary Antibody, Alkaline Phosphatase (Pre-adsorbed), Novus Biologicals™
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Donkey Polyclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Sheep |
| Host Species | Donkey |
| Conjugate | Alkaline Phosphatase (AP) |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | 1 mg/mL |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:500 - 1:2500, ELISA 1:2000 - 1:10000, Immunohistochemistry 1:200 - 1:1000 |
| Classification | Polyclonal |
| Immunogen | Sheep IgG whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | This antibody was pre-adsorbed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, and Rat Serum Proteins. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins. |
Goat F(ab')2 anti-Mouse IgG F(ab')2 Secondary Antibody, Rhodamine (Pre-adsorbed), Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Mouse |
| Host Species | Goat |
| Conjugate | Rhodamine |
| Applications | Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence |
| Isotype | IgG |
| Antigen | IgG F(ab')2 |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000 |
| Gene Alias | immunoglobulin heavy constant gamma 1 (G1m marker) |
| Classification | Polyclonal |
| Immunogen | Mouse IgG F(ab)2 fragment |
| Primary or Secondary | Secondary |
| Test Specificity | No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. |
Goat anti-Rat IgG (H+L) Secondary Antibody, Rhodamine, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Rat |
| Host Species | Goat |
| Conjugate | Rhodamine |
| Applications | Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot |
| Form | Purified |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Multi-step |
| Dilution | Western Blot 1:1000 - 1:10000, Flow Cytometry 1:500 - 1:2500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000 |
| Classification | Polyclonal |
| Immunogen | Rat IgG whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. |
Goat anti-Guinea Pig IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Guinea Pig |
| Host Species | Goat |
| Conjugate | HRP |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Form | Purified |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | 1.0 mg/mL |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Multi-step |
| Dilution | Western Blot 1:1000 - 1:5000, ELISA 1:100000 - 1:200000, Immunohistochemistry 1:500 - 1:2500 |
| Classification | Polyclonal |
| Immunogen | Goat anti-Guinea Pig IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) was produced by repeated immunization with Guinea Pig IgG whole molecule in goat. |
| Reconstitution | Reconstitute with deionized water (or equivalent) to desired concetration. |
| Primary or Secondary | Secondary |
| Test Specificity | This antibody was pre-adsorbed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat, and Sheep Serum Proteins. No reaction was observed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. |
Donkey anti-Goat IgG (H+L) Secondary Antibody, Texas Red, Novus Biologicals™
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Donkey Polyclonal Antibody
| Content And Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Goat |
| Host Species | Donkey |
| Conjugate | Texas Red |
| Applications | Dot Blot,Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Form | Purified |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | 2.0 mg/mL |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Multi-step |
| Dilution | Western Blot 0.04-0.4 ug/ml, Flow Cytometry 1:500 - 1:2500, Immunohistochemistry 1:200 - 1:500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Immunohistochemistry-Paraffin 1:200 - 1:500, Dot Blot, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000 |
| Classification | Polyclonal |
| Immunogen | Goat IgG whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum. |
Sheep anti-Human IgG (H+L) Secondary Antibody, Rhodamine, Novus Biologicals™
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Sheep Polyclonal Antibody
| Content And Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Human |
| Host Species | Sheep |
| Conjugate | Rhodamine |
| Applications | Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence |
| Form | Purified |
| Isotype | IgG |
| Antigen | IgG |
| Regulatory Status | RUO |
| Purification Method | Multi-step |
| Dilution | Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000 |
| Gene Alias | immunoglobulin heavy constant gamma 1 (G1m marker) |
| Classification | Polyclonal |
| Immunogen | Human IgG whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum, Human IgG and Human Serum. |