Secondary Antibodies
Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies
(44,180)
Mouse Secondary Antibodies
(14,490)
Rabbit Secondary Antibodies
(6,301)
Donkey Secondary Antibodies
(4,319)
Rat Secondary Antibodies
(1,234)
Sheep Secondary Antibodies
(701)
Chicken Secondary Antibodies
(403)
Bovine Secondary Antibodies
(257)
Pig Secondary Antibodies
(76)
Horse Secondary Antibodies
(74)
Alpaca Secondary Antibodies
(62)
Hamster Secondary Antibodies
(57)
Biosimilar Secondary Antibodies
(9)
Human Secondary Antibodies
(7)
Duck Secondary Antibodies
(3)
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Rabbit anti-Guinea Pig IgG (H+L) Secondary Antibody, Allophycocyanin, Novus Biologicals™
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Rabbit Polyclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Guinea Pig |
| Host Species | Rabbit |
| Conjugate | APC |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Form | Purified |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Immunogen affinity purified |
| Formulation | PBS |
| Classification | Polyclonal |
| Immunogen | Guinea Pig IgG whole molecule |
| Primary or Secondary | Secondary |
Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody, Alkaline Phosphatase (Pre-adsorbed), Novus Biologicals™
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Rabbit Polyclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Mouse |
| Host Species | Rabbit |
| Conjugate | Alkaline Phosphatase (AP) |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Concentration | 1 mg/mL |
| Antigen | IgG F(ab')2 |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:500 - 1:2500, ELISA 1:2000 - 1:10000, Immunohistochemistry 1:200 - 1:1000 |
| Gene Alias | immunoglobulin heavy constant gamma 1 (G1m marker) |
| Classification | Polyclonal |
| Immunogen | Mouse IgG F(ab')2 fragment |
| Primary or Secondary | Secondary |
| Test Specificity | No reactivity was observed against Mouse IgG F(c) or Human serum proteins. |
| Content And Storage | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
|---|---|
| Target Species | Pig |
| Host Species | Rabbit |
| Conjugate | HRP |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Form | Liquid |
| Isotype | IgG |
| Concentration | 2 mg/mL |
| Antigen | Pig IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | IgG fraction |
| Product Type | Secondary Antibody |
| Formulation | 20mM potassium phosphate with 1% BSA, 150mM NaCl and 0.01% gentamicin sulfate; pH 7.2 |
| Immunogen | Pig IgG whole molecule. |
| Classification | Polyclonal |
| Primary or Secondary | Secondary |
Rabbit anti-Hamster IgG (H+L) Secondary Antibody (Pre-adsorbed), Novus Biologicals™
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Rabbit Polyclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Hamster |
| Host Species | Rabbit |
| Conjugate | Unconjugated |
| Applications | Chemiluminescence Immunoassay (CIA),ELISA,Flow Cytometry,Immunoassay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunomicroscopy,Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | 4.5mg/mL |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Immunoassay |
| Classification | Polyclonal |
| Immunogen | Purified Hamster IgG (H&L) |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with heavy gamma chains on hamster IgG and light chains on all hamster immunoglobulins. This antibody has been pre-adsorbed against human serum proteins. |
IgG Rabbit anti-Llama, HRP, Secondary Antibody, Novus Biologicals™
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Rabbit Polyclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Conjugate | HRP |
| Target Species | Llama |
| Host Species | Rabbit |
| Applications | Western Blot |
| Form | Purified |
| Antigen | IgG |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Formulation | PBS |
| Gene ID (Entrez) | 3500 |
| Classification | Polyclonal |
| Immunogen | Purified Llama IgG |
| Primary or Secondary | Secondary |
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IgD Antibody (RM123), Biotin, Novus Biologicals™
IgD Antibody (RM123), Biotin, Novus Biologicals™
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Rabbit Monoclonal Antibody
| Content And Storage | Store at -20°C. Avoid freeze-thaw cycles. |
|---|---|
| Conjugate | Biotin |
| Target Species | Human |
| Host Species | Rabbit |
| Applications | ELISA,Immunohistochemistry,Sandwich ELISA Capture,Sandwich ELISA Detection |
| Form | Purified |
| Isotype | IgG |
| Antigen | IgD |
| Purification Method | Protein A purified |
| Gene Alias | IgD, Igh-5, Ighd, Immunoglobulin D, Immunoglobulin Delta |
| Formulation | PBS, 50% Glycerol, 1% BSA with 0.09% Sodium Azide |
| Gene ID (Entrez) | 3495 |
| Classification | Monoclonal |
| Immunogen | Human IgD |
| Primary or Secondary | Secondary |
| Clone | RM123 |
MilliporeSigma™ Rabbit anti-Mouse IgG2a kappa light chain, Clone: RM107, Secondary Antibody,
Rabbit Monoclonal Antibody
| Content And Storage | 2°C to 8°C for one year from date of shipment |
|---|---|
| Host Species | Rabbit |
| Applications | ELISA,Western Blot |
| Form | Purified |
| Isotype | IgG |
| Antigen | IgG2a kappa light chain |
| Regulatory Status | RUO |
| Purification Method | Protein A purified |
| Formulation | Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide. |
| Immunogen | Mouse IgG |
| Classification | Monoclonal |
| Primary or Secondary | Secondary |
| Clone | RM107 |
| Test Specificity | This antibody reacts to the Fab region of mouse IgG2aκ. No cross reactivity with mouse IgG2aγ, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The Fc region of RM107 has been engineered to eliminate the binding of Fc receptor. |
Invitrogen™ Rabbit anti-Mouse IgG (H+L) Secondary Antibody, TRITC
Rabbit Polyclonal Secondary Antibody
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Content And Storage | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
|---|---|
| Target Species | Mouse |
| Host Species | Rabbit |
| Conjugate | TRITC |
| Applications | Immunocytochemistry |
| Form | Lyophilized |
| Isotype | IgG |
| Concentration | 1 mg/mL |
| Antigen | Mouse IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity chromatography |
| Product Type | Secondary Antibody |
| Formulation | PBS with 1% BSA and 0.05% sodium azide; pH 7.2 |
| Immunogen | Gamma Immunoglobins Heavy and Light chains. |
| Classification | Polyclonal |
| Primary or Secondary | Secondary |
Rabbit anti-Goat IgG (H+L) Secondary Antibody, Rhodamine, Novus Biologicals™
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Rabbit Polyclonal Antibody
| Content And Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Goat |
| Host Species | Rabbit |
| Conjugate | Rhodamine |
| Applications | Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence |
| Form | Purified |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Immunogen affinity purified |
| Dilution | Flow Cytometry, Immunocytochemistry/Immunofluorescence 1:500 - 1:2500, Fluorophore-linked immunosorbent assay 1:100 - 1:2000 |
| Classification | Polyclonal |
| Immunogen | Goat IgG whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | Prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Rabbit Serum, Goat IgG and Goat Serum. |
Rabbit anti-Mouse IgG Fc Secondary Antibody, Rhodamine, Novus Biologicals™
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Rabbit Polyclonal Antibody
| Content And Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Mouse |
| Host Species | Rabbit |
| Conjugate | Rhodamine |
| Applications | Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence |
| Isotype | IgG |
| Concentration | LYOPH |
| Antigen | IgGFc |
| Purification Method | Affinity Purified |
| Dilution | Flow Cytometry 1:500 - 1:2500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000 |
| Formulation | Lyophilized from 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, with 10Mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free with 0.01% Sodium Azide |
| Classification | Polyclonal |
| Immunogen | Mouse IgG Fc fragment |
| Reconstitution | Reconstitute with 1.0mL deionized water. |
| Primary or Secondary | Secondary |
| Test Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab). |
Rabbit anti-Feline IgG Fc Secondary Antibody, FITC, Novus Biologicals™
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Rabbit Polyclonal Antibody
| Content And Storage | Store at 4C in the dark. |
|---|---|
| Target Species | Feline |
| Host Species | Rabbit |
| Conjugate | Fluorescein |
| Applications | Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence |
| Isotype | IgG |
| Concentration | LYOPH |
| Antigen | IgG Fc |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000 |
| Classification | Polyclonal |
| Immunogen | Cat IgG F(c) fragment |
| Reconstitution | Reconstitute with 1.0 ml deionized water (or equivalent). |
| Primary or Secondary | Secondary |
| Test Specificity | No reaction was observed against Cat IgG F(ab). |
Rabbit anti-Bovine IgA Heavy Chain Secondary Antibody, Novus Biologicals™
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Rabbit Polyclonal Antibody has been used in 3 publications
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Bovine |
| Host Species | Rabbit |
| Conjugate | Unconjugated |
| Applications | ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Isotype | IgA |
| Concentration | 1.0 mg/mL |
| Antigen | IgA Heavy Chain |
| Gene Symbols | IGHA1 |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:1000-1:10000, ELISA, Immunohistochemistry 1:200 - 1:2000, Immunocytochemistry/Immunofluorescence 1:200 - 1:2000, Immunohistochemistry-Paraffin |
| Gene Alias | Immunoglobulin A |
| Gene ID (Entrez) | 3493 |
| Classification | Polyclonal |
| Immunogen | Bovine IgA |
| Primary or Secondary | Secondary |
| Test Specificity | By immunoelectrophoresis and ELISA this reacts specifically with bovine IgA. This may cross react with IgA from other species. |
Rabbit anti-Sheep IgG F(ab')2 Secondary Antibody, Rhodamine (Pre-adsorbed), Novus Biologicals™
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Rabbit Polyclonal Antibody
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Sheep |
| Host Species | Rabbit |
| Conjugate | Rhodamine |
| Applications | Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence |
| Isotype | IgG |
| Concentration | LYOPH |
| Antigen | IgG F(ab')2 |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000 |
| Gene Alias | immunoglobulin heavy constant gamma 1 (G1m marker) |
| Classification | Polyclonal |
| Immunogen | Sheep IgG F(ab')2 fragment |
| Reconstitution | Reconstitute with 500 ul deionized water (or equivalent). |
| Primary or Secondary | Secondary |
| Test Specificity | No reaction was observed against Sheep IgG F(c) or Human Serum Proteins. |
Rabbit anti-Sheep IgA Secondary Antibody, Janelia Fluor 646, Novus Biologicals™
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Rabbit Polyclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Conjugate | Janelia Fluor 646 |
| Target Species | Sheep |
| Host Species | Rabbit |
| Applications | ELISA |
| Form | Purified |
| Isotype | IgG |
| Antigen | IgA |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Gene Alias | Immunoglobulin A |
| Formulation | 50mM Sodium Borate |
| Gene ID (Entrez) | 3493 |
| Classification | Polyclonal |
| Immunogen | Ovine IgA |
| Primary or Secondary | Secondary |
Rabbit anti-Human IgG1 Fc Secondary Antibody (245), Janelia Fluor 646, Novus Biologicals™
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Rabbit Monoclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Human |
| Host Species | Rabbit |
| Conjugate | Janelia Fluor 646 |
| Applications | ELISA |
| Form | Purified |
| Isotype | IgG |
| Antigen | IgG1 Fc |
| Regulatory Status | RUO |
| Purification Method | Protein A purified |
| Formulation | 50mM Sodium Borate |
| Classification | Monoclonal |
| Immunogen | This antibody was obtained from a rabbit immunized with purified, recombinant Human IgG1 Fc (Uniprot#: P01857-1; Glu99-Lys330,C103S). HEK293 used as expression system. |
| Primary or Secondary | Secondary |
| Clone | 245 |