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Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies (44,218)
Mouse Secondary Antibodies (14,540)
Rabbit Secondary Antibodies (6,314)
Donkey Secondary Antibodies (4,319)
Rat Secondary Antibodies (1,238)
Sheep Secondary Antibodies (701)
Chicken Secondary Antibodies (403)
Bovine Secondary Antibodies (258)
Pig Secondary Antibodies (78)
Horse Secondary Antibodies (74)
Alpaca Secondary Antibodies (62)
Hamster Secondary Antibodies (57)
Biosimilar Secondary Antibodies (9)
Human Secondary Antibodies (7)
Duck Secondary Antibodies (3)

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7,411 – 7,425 of 72,086 results
7,411

Rabbit anti-Human IgG1 Fc Secondary Antibody (245), FITC, Novus Biologicals™
SDP

Rabbit Monoclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Human
Host Species Rabbit
Conjugate FITC
Applications ELISA
Form Purified
Isotype IgG
Antigen IgG1 Fc
Regulatory Status RUO
Purification Method Protein A purified
Formulation PBS
Classification Monoclonal
Immunogen This antibody was obtained from a rabbit immunized with purified, recombinant Human IgG1 Fc (Uniprot#: P01857-1; Glu99-Lys330,C103S). HEK293 used as expression system.
Primary or Secondary Secondary
Clone 245
7,412

Rabbit anti-Human IgG Fc Secondary Antibody, HRP, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Human
Host Species Rabbit
Conjugate HRP
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Concentration LYOPH
Antigen IgG Fc
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:1000 - 1:5000, ELISA 1:10000 - 1:50000, Immunohistochemistry 1:500 - 1:2500
Classification Polyclonal
Immunogen Rabbit anti-Human IgG Fc fragment Secondary Antibody was produced by repeated immunization with Human IgG F(c) fragment in rabbit.
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity No reaction was observed against Human IgG F(ab).
7,413

Rabbit anti-Canine IgG Fc Secondary Antibody, PerCP, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Antigen IgG Fc
Regulatory Status RUO
Content And Storage Store at 4°C in the dark.
Purification Method Multi-step
Target Species Canine
Host Species Rabbit
Conjugate PerCP
Applications Dot Blot,ELISA,Immunohistochemistry,Western Blot
Formulation PBS
Classification Polyclonal
Immunogen Dog IgG F(c) fragment
Isotype IgG
Primary or Secondary Secondary
7,414

Invitrogen™ Rabbit anti-Mouse IgG (H+L) Secondary Antibody, AP

Rabbit Polyclonal Secondary Antibody

ENCOMPASS_PREFERRED
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target Species Mouse
Host Species Rabbit
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Western Blot
Form Liquid
Isotype IgG
Concentration 1.5 mg/mL
Antigen Mouse IgG (H+L)
Regulatory Status RUO
Purification Method Affinity chromatography
Product Type Secondary Antibody
Formulation Secondary antibody with 5mM MgCl2, 0.1mM ZnCl2, 1% BSA, 30mM triethanolamine and 0.05% sodium azide; pH 7.2
Immunogen Gamma Immunoglobins Heavy and Light chains.
Classification Polyclonal
Primary or Secondary Secondary
7,416

Rabbit anti-Guinea Pig IgG (H+L) Secondary Antibody, Allophycocyanin, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Guinea Pig
Host Species Rabbit
Conjugate APC
Applications ELISA,Immunohistochemistry,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation PBS
Classification Polyclonal
Immunogen Guinea Pig IgG whole molecule
Primary or Secondary Secondary
7,417

Rabbit anti-Rat IgM Heavy Chain Secondary Antibody, Rhodamine, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Rat
Host Species Rabbit
Conjugate Rhodamine
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgM
Concentration LYOPH
Antigen IgM Heavy Chain
Gene Symbols IGHM
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:1000 - 1:10000, Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Gene Alias Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain
Gene ID (Entrez) 3507
Classification Polyclonal
Immunogen Rat IgM heavy chain
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity No reaction was observed against other rat heavy or light chain proteins.
7,418

Rabbit anti-Mouse IgM (H+L) Secondary Antibody, FITC, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Mouse
Host Species Rabbit
Conjugate FITC
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence
Form Purified
Concentration LYOPH
Antigen IgM(H+L)
Purification Method Ion exchange chromatography
Dilution Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Formulation 0.01M Sodium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
Classification Polyclonal
Immunogen Mouse IgM, whole molecule
Reconstitution Reconstitute with 0.1mL deionized water.
Primary or Secondary Secondary
Test Specificity This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgM, and Mouse Serum. Some light chain cross reactivity with Mouse IgG may occur.
7,419

Rabbit anti-Goat IgG (H+L) Secondary Antibody, HRP, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody has been used in 6 publications

Content And Storage Store at 4C. Do not freeze.
Target Species Goat
Host Species Rabbit
Conjugate HRP
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0 mg/mL
Antigen IgG (H+L)
Gene Symbols IGHG
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:5000 - 1:50000, ELISA 1:10000-1:100000, Immunohistochemistry 1:100 - 1:500, Immunocytochemistry/Immunofluorescence 1:100 - 1:500, Immunohistochemistry-Paraffin 1:200 - 1:5000
Classification Polyclonal
Immunogen Goat IgG-heavy and light chain
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
7,420

Invitrogen™ Rabbit anti-Mouse IgG (H+L) Secondary Antibody, AP

Rabbit Polyclonal Secondary Antibody

Content And Storage 4°C
Target Species Mouse
Host Species Rabbit
Conjugate Alkaline Phosphatase (AP)
Applications Western Blot
Form Lyophilized
Isotype IgG
Concentration 0.6 mg/mL
Antigen Mouse IgG (H+L)
Regulatory Status RUO
Purification Method Affinity chromatography
Product Type Secondary Antibody
Formulation TBS with 15mg/mL BSA and 0.05% sodium azide; pH 8
Classification Polyclonal
Primary or Secondary Secondary
7,421

Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody, Allophycocyanin, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Antigen IgG1 Heavy Chain
Regulatory Status RUO
Content And Storage Store at 4°C in the dark.
Purification Method Multi-step
Target Species Mouse
Host Species Rabbit
Conjugate APC
Applications Dot Blot,ELISA,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Formulation PBS
Classification Polyclonal
Immunogen Mouse IgG1 heavy chain
Isotype IgG
Primary or Secondary Secondary
7,422

Rabbit anti-Chicken IgG Fc Secondary Antibody, Texas Red, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Chicken
Host Species Rabbit
Conjugate Texas Red
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgG
Concentration LYOPH
Antigen IgG Fc
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:1000 - 1:10000, Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Classification Polyclonal
Immunogen Chicken IgG F(c) fragment
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity No reaction was observed against Chicken IgG F(ab).
7,423

Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody, Alkaline Phosphatase (Pre-adsorbed), Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Mouse
Host Species Rabbit
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Concentration 1 mg/mL
Antigen IgG F(ab')2
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:500 - 1:2500, ELISA 1:2000 - 1:10000, Immunohistochemistry 1:200 - 1:1000
Gene Alias immunoglobulin heavy constant gamma 1 (G1m marker)
Classification Polyclonal
Immunogen Mouse IgG F(ab')2 fragment
Primary or Secondary Secondary
Test Specificity No reactivity was observed against Mouse IgG F(c) or Human serum proteins.
7,424

Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody, DyLight 680, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Mouse
Host Species Rabbit
Conjugate DyLight 680
Applications Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgG2b
Concentration LYOPH
Antigen IgG2b Heavy Chain
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot, Fluorophore-linked immunosorbent assay
Classification Polyclonal
Immunogen Mouse IgG2b chain
Reconstitution Reconstitute with 100 ul deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity Minimal cross reactivity was noted against other Mouse immunoglobulin classes or subclasses.
7,425

Rabbit anti-Goat IgG (H+L) Secondary Antibody [Janelia Fluor 669], Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Goat
Host Species Rabbit
Conjugate Janelia Fluor 669
Applications Western Blot,ELISA,Immunohistochemistry
Form Purified
Isotype IgG
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Goat IgG whole molecule
Primary or Secondary Secondary