Secondary Antibodies
Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies
(44,725)
Mouse Secondary Antibodies
(14,931)
Rabbit Secondary Antibodies
(6,305)
Donkey Secondary Antibodies
(4,350)
Rat Secondary Antibodies
(1,301)
Sheep Secondary Antibodies
(706)
Chicken Secondary Antibodies
(404)
Bovine Secondary Antibodies
(258)
Horse Secondary Antibodies
(77)
Pig Secondary Antibodies
(77)
Alpaca Secondary Antibodies
(62)
Hamster Secondary Antibodies
(61)
Biosimilar Secondary Antibodies
(9)
Human Secondary Antibodies
(7)
Duck Secondary Antibodies
(3)
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| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Rabbit |
| Host Species | Goat |
| Conjugate | PerCP |
| Applications | ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Form | Purified |
| Isotype | IgG |
| Antigen | IgG Fc |
| Regulatory Status | RUO |
| Purification Method | Immunogen affinity purified |
| Formulation | PBS |
| Classification | Polyclonal |
| Immunogen | Rabbit IgG-Fc Fragment |
| Primary or Secondary | Secondary |
Goat anti-Canine IgG (H+L) Secondary Antibody, DyLight 650, Novus Biologicals™
Goat Polyclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Canine |
| Host Species | Goat |
| Conjugate | DyLight 650 |
| Applications | ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Form | Purified |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Immunogen affinity purified |
| Formulation | 50mM Sodium Borate |
| Classification | Polyclonal |
| Immunogen | This Goat anti-Canine IgG (H+L) Secondary Antibody was developed against canine IgG-heavy and light chain. |
| Primary or Secondary | Secondary |
Goat F(ab) anti-Rabbit IgG (H+L) Secondary Antibody, FITC, Novus Biologicals™
Goat Polyclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Rabbit |
| Host Species | Goat |
| Conjugate | FITC |
| Applications | Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot |
| Isotype | IgG |
| Concentration | LYOPH |
| Antigen | IgG (H+L) |
| Purification Method | Affinity Purified |
| Dilution | Western Blot, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000 |
| Formulation | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Classification | Polyclonal |
| Immunogen | Rabbit IgG whole molecule |
| Reconstitution | Reconstitute with 1.0 mL deionized water |
| Test Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c). |
Goat anti-Equine IgG (H+L) Secondary Antibody, DyLight 594, Novus Biologicals™
Goat Polyclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Equine |
| Host Species | Goat |
| Conjugate | DyLight 594 |
| Applications | ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Form | Purified |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Immunogen affinity purified |
| Formulation | 50mM Sodium Borate |
| Classification | Polyclonal |
| Immunogen | Horse IgG(T) |
| Primary or Secondary | Secondary |
Goat anti-Human IgE Heavy Chain Secondary Antibody, PerCP, Novus Biologicals™
Goat Polyclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Human |
| Host Species | Goat |
| Conjugate | PerCP |
| Applications | ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Form | Purified |
| Isotype | IgG |
| Antigen | IgE Heavy Chain |
| Regulatory Status | RUO |
| Purification Method | Immunogen affinity purified |
| Gene Alias | Constant Region of Heavy Chain of IgE, Ig Epsilon Chain C Region, Ig Epsilon Chain C Region ND, IgE, Immunoglobulin Epsilon, immunoglobulin heavy constant epsilon |
| Formulation | PBS |
| Classification | Polyclonal |
| Immunogen | Human IgE |
| Primary or Secondary | Secondary |
Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 405, Novus Biologicals™
Goat Polyclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Rabbit |
| Host Species | Goat |
| Conjugate | DyLight 405 |
| Applications | ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Form | Purified |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Immunogen affinity purified |
| Formulation | 50mM Sodium Borate |
| Classification | Polyclonal |
| Immunogen | This Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against rabbit IgG. |
| Primary or Secondary | Secondary |
Goat anti-Mouse IgM Heavy Chain Secondary Antibody, DyLight 405, Novus Biologicals™
Goat Polyclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Conjugate | DyLight 405 |
| Target Species | Mouse |
| Host Species | Goat |
| Applications | ELISA |
| Form | Purified |
| Isotype | IgG |
| Antigen | IgM Heavy Chain |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Gene Alias | Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain |
| Formulation | 50mM Sodium Borate |
| Gene ID (Entrez) | 3507 |
| Classification | Polyclonal |
| Immunogen | Mouse IgM mu heavy chain |
| Primary or Secondary | Secondary |
Promotions Available
Amersham™ CyDye™ 800 Goat-Anti-Mouse Secondary Antibody, Cytiva
Amersham™ CyDye™ 800 Goat-Anti-Mouse Secondary Antibody, Cytiva
Amersham™ CyDye™ NIR-labeled secondary antibodies are designed for quantitative fluorescent Western blotting.
| Content And Storage | 2°C to 8°C, protected from light |
|---|---|
| Conjugate | CyDye 800 |
| Applications | Western Blot |
| Primary or Secondary | Secondary |
Goat anti-Rat IgG (H+L) Secondary Antibody, Rhodamine (Pre-adsorbed), Novus Biologicals™
Goat Polyclonal Antibody
| Content And Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Rat |
| Host Species | Goat |
| Conjugate | Rhodamine |
| Applications | Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot |
| Form | Purified |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | 1.0 mg/mL |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Multi-step |
| Dilution | Western Blot 1:1000 - 1:10000, Flow Cytometry 1:500 - 1:2500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000 |
| Classification | Polyclonal |
| Immunogen | Rat IgG whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | This antibody was pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, and Sheep Serum Proteins. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, and Sheep Serum Proteins. |
Goat anti-Human IgG (H+L) Secondary Antibody, Janelia Fluor 549, Novus Biologicals™
Goat Polyclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Human |
| Host Species | Goat |
| Conjugate | Janelia Fluor 549 |
| Applications | ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Form | Purified |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Immunogen affinity purified |
| Formulation | 50mM Sodium Borate |
| Classification | Polyclonal |
| Immunogen | This Goat anti-Human IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against human IgG. |
| Primary or Secondary | Secondary |
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Rat |
| Host Species | Goat |
| Conjugate | DyLight 550 |
| Applications | ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Form | Purified |
| Isotype | IgG |
| Antigen | IgG Fc |
| Regulatory Status | RUO |
| Purification Method | Immunogen affinity purified |
| Formulation | 50mM Sodium Borate |
| Classification | Polyclonal |
| Immunogen | Rat IgG-Fc Fragment |
| Primary or Secondary | Secondary |
| Content And Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Human |
| Host Species | Goat |
| Conjugate | HRP |
| Applications | Dot Blot,ELISA,Immunohistochemistry,Western Blot |
| Form | Purified |
| Isotype | IgG |
| Antigen | IgG |
| Regulatory Status | RUO |
| Purification Method | Multi-step |
| Dilution | Western Blot 1:5000 - 1:25000, ELISA 1:20000 - 1:80000, Immunohistochemistry 1:1000 - 1:5000, Dot Blot |
| Gene Alias | immunoglobulin heavy constant gamma 1 (G1m marker) |
| Classification | Polyclonal |
| Immunogen | Goat anti-Human IgG (H+L) Secondary Antibody [HRP] was produced by repeated immunization with human IgG whole molecule in goat. |
| Reconstitution | Reconstitute with deionized water (or equivalent) to desired concetration. |
| Primary or Secondary | Secondary |
| Test Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Peroxidase, anti-Goat Serum, Human IgG and Human Serum. |
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Mouse |
| Host Species | Goat |
| Conjugate | PE |
| Applications | ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Form | Purified |
| Isotype | IgG |
| Antigen | IgG Fc |
| Regulatory Status | RUO |
| Purification Method | Immunogen affinity purified |
| Formulation | PBS |
| Classification | Polyclonal |
| Immunogen | Mouse IgG-Fc |
| Primary or Secondary | Secondary |
Goat anti-Canine IgA Heavy Chain Secondary Antibody, FITC, Novus Biologicals™
Goat Polyclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Canine |
| Host Species | Goat |
| Conjugate | FITC |
| Applications | Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin) |
| Isotype | IgG |
| Concentration | 1.0 mg/mL |
| Antigen | IgA Heavy Chain |
| Gene Symbols | IGHA1 |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:200 - 1:2, Immunohistochemistry-Paraffin 1:200 - 1:2 |
| Gene Alias | Immunoglobulin A |
| Gene ID (Entrez) | 3493 |
| Classification | Polyclonal |
| Immunogen | Dog IgA |
| Primary or Secondary | Secondary |
| Test Specificity | By immunoelectrophoresis and ELISA this reacts specifically with dog IgA. This may cross react with IgA from other species. |