Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).

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1,276 – 1,290 of 73,088 results

1,276

Donkey anti-Goat IgG (H+L) Secondary Antibody, Novus Biologicals™

Donkey Polyclonal Antibody has been used in 8 publications

Pricing & Availability
Specifications

Content And Storage	Store at 4C. Do not freeze.
Target Species	Goat
Host Species	Donkey
Conjugate	Unconjugated
Applications	Adhesion Activation,ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Frozen),Immunohistochemistry (Paraffin),Western Blot
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Concentration	1.0 mg/mL
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Affinity Purified
Dilution	Western Blot 1:1, ELISA 1:1, Immunohistochemistry 1:200-1:2000, Immunocytochemistry/Immunofluorescence 1:200 - 1:2, Immunohistochemistry-Paraffin 1:200 - 1:2000, Immunohistochemistry-Frozen 1:200-1:2000, Adhesion Activation
Classification	Polyclonal
Immunogen	Goat IgG-heavy and light chain
Primary or Secondary	Secondary
Test Specificity	By immunoelectrophoresis and ELISA this reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.

1,277

Donkey anti-Mouse IgG (H+L) Secondary Antibody, DyLight 350, Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C in the dark.
Target Species	Mouse
Host Species	Donkey
Conjugate	DyLight 350
Applications	ELISA,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Western Blot
Form	Purified
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Multi-step
Formulation	50mM Sodium Borate
Classification	Polyclonal
Immunogen	Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) was produced by repeated immunization with Mouse IgG whole molecule in donkey.
Primary or Secondary	Secondary

1,278

Donkey anti-Rat IgG (H+L) Secondary Antibody, FITC, Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4C in the dark.
Target Species	Rat
Host Species	Donkey
Conjugate	Fluorescein
Applications	Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Concentration	LYOPH
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Affinity Purified
Dilution	Western Blot 1:1000 - 1:10000, Flow Cytometry 1:500 - 1:2500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Classification	Polyclonal
Immunogen	Rat IgG whole molecule
Reconstitution	Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary	Secondary
Test Specificity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Rat IgG and Rat Serum.

1,279

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 488, Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C in the dark.
Target Species	Rabbit
Host Species	Donkey
Conjugate	DyLight 488
Applications	ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form	Purified
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Immunogen affinity purified
Formulation	50mM Sodium Borate
Classification	Polyclonal
Immunogen	This Donkey anti-Rabbit IgG (H+L) Secondary Antibody was developed against rabbit IgG-heavy and light chain.
Primary or Secondary	Secondary

1,280

Donkey anti-Sheep IgG (H+L) Secondary Antibody, Biotin (Pre-adsorbed), Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4C. Do not freeze.
Target Species	Sheep
Host Species	Donkey
Conjugate	Biotin
Applications	ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Concentration	1.0mg/mL
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Affinity purified
Dilution	Western Blot 1:2000 - 1:20000, ELISA 1:2000 - 1:20000, Immunohistochemistry 1:500 - 1:5000, Immunohistochemistry-Paraffin
Classification	Polyclonal
Immunogen	Purified Sheep IgG (H&L)
Primary or Secondary	Secondary
Test Specificity	Based on IEP, this antibody reacts with heavy gamma chains on sheep IgG and light chains on all sheep immunoglobulins. This antibody has been pre-adsorbed against human and rabbit IgG.

1,281

Donkey anti-Human IgG (H+L) Secondary Antibody, DyLight 680, Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C in the dark.
Target Species	Human
Host Species	Donkey
Conjugate	DyLight 680
Applications	ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form	Purified
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Immunogen affinity purified
Formulation	50mM Sodium Borate
Classification	Polyclonal
Immunogen	This Donkey anti-Human IgG (H+L) Secondary Antibody was developed against human IgG-heavy and light chain.
Primary or Secondary	Secondary

1,282

Donkey anti-Rat IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species	Rat
Host Species	Donkey
Conjugate	HRP
Applications	ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Concentration	LYOPH
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Affinity purified
Dilution	Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification	Polyclonal
Immunogen	Purified Rat IgG (H&L)
Reconstitution	Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary	Secondary
Test Specificity	Based on IEP, this antibody reacts with heavy gamma chains on rat IgG and light chains on all rat immunoglobulins. This antibody has been pre-adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit and sheep IgG.

1,283

Donkey anti-Mouse IgG (H+L) Secondary Antibody, FITC, Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Species	Mouse
Host Species	Donkey
Conjugate	Fluorescein
Applications	Flow Cytometry,Immunocytochemistry/Immunofluorescence,Western Blot
Form	Purified
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Multi-step
Dilution	Western Blot 1:1000 - 1:10000, Flow Cytometry, Immunocytochemistry/Immunofluorescence 1:500 - 1:2500
Classification	Polyclonal
Immunogen	Mouse IgG whole molecule
Primary or Secondary	Secondary
Test Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Mouse IgG and Mouse Serum.

1,284

Donkey anti-Sheep IgG (H+L) Secondary Antibody, FITC (Pre-adsorbed), Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4C in the dark.
Target Species	Sheep
Host Species	Donkey
Conjugate	Fluorescein
Applications	Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Concentration	LYOPH
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Affinity Purified
Dilution	Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Classification	Polyclonal
Immunogen	Sheep IgG whole molecule
Reconstitution	Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary	Secondary
Test Specificity	This antibody was pre-adsorbed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, and Rat Serum Proteins. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbits and Rat Serum Proteins.

1,285

Invitrogen™ Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Biotin

Donkey Polyclonal Secondary Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target Species	Rat
Host Species	Donkey
Conjugate	Biotin
Applications	ELISA,Flow Cytometry,Western Blot,Immunocytochemistry
Form	Liquid
Isotype	IgG
Concentration	1.5 mg/mL
Antigen	Rat IgG (H+L) Highly Cross-Adsorbed
Regulatory Status	RUO
Purification Method	Affinity chromatography
Product Type	Secondary Antibody
Formulation	PBS with 1% BSA and 0.05% sodium azide; pH 7.2
Immunogen	Gamma Immunoglobins Heavy and Light chains.
Classification	Polyclonal
Primary or Secondary	Secondary

1,286

Donkey anti-Sheep IgG (H+L) Secondary Antibody, Rhodamine, Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Species	Sheep
Host Species	Donkey
Conjugate	Rhodamine
Applications	Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry
Form	Purified
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Immunogen affinity purified
Dilution	Flow Cytometry 1:500 - 1:2500, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Classification	Polyclonal
Immunogen	Sheep IgG whole molecule
Primary or Secondary	Secondary
Test Specificity	Sheep IgG (H and L)

1,287

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, PE, Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C in the dark.
Target Species	Rabbit
Host Species	Donkey
Conjugate	PE
Applications	ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form	Purified
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Immunogen affinity purified
Formulation	PBS
Classification	Polyclonal
Immunogen	This Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against rabbit IgG.
Primary or Secondary	Secondary

1,288

Donkey anti-Mouse IgG (H+L) Secondary Antibody, Biotin (Pre-adsorbed), Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Species	Mouse
Host Species	Donkey
Conjugate	Biotin
Applications	ELISA,Immunohistochemistry,Western Blot
Form	Purified
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Concentration	1.0 mg/mL
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Multi-step
Dilution	Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000
Classification	Polyclonal
Immunogen	Mouse IgG whole molecule
Primary or Secondary	Secondary
Test Specificity	This antibody was pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat, and Sheep Serum Proteins. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

1,289

Donkey anti-Mouse IgG (H+L) Secondary Antibody, Allophycocyanin, Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C in the dark.
Target Species	Mouse
Host Species	Donkey
Conjugate	APC
Applications	ELISA,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Western Blot
Form	Purified
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Multi-step
Formulation	PBS
Classification	Polyclonal
Immunogen	Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) was produced by repeated immunization with Mouse IgG whole molecule in donkey.
Primary or Secondary	Secondary

1,290

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Biotin, Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Species	Rabbit
Host Species	Donkey
Conjugate	Biotin
Applications	ELISA,Immunohistochemistry,Western Blot
Form	Purified
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Multi-step
Dilution	Western Blot 1:3000 - 1:15000, ELISA 1:300000, Immunohistochemistry 1:500 - 1:3000
Classification	Polyclonal
Immunogen	Rabbit IgG whole molecule
Primary or Secondary	Secondary
Test Specificity	Rabbit IgG (H and L)

Secondary Antibodies

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