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Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies (44,275)
Mouse Secondary Antibodies (14,507)
Rabbit Secondary Antibodies (6,327)
Donkey Secondary Antibodies (4,321)
Rat Secondary Antibodies (1,240)
Sheep Secondary Antibodies (703)
Chicken Secondary Antibodies (403)
Bovine Secondary Antibodies (257)
Horse Secondary Antibodies (76)
Pig Secondary Antibodies (76)
Alpaca Secondary Antibodies (62)
Hamster Secondary Antibodies (55)
Biosimilar Secondary Antibodies (9)
Human Secondary Antibodies (7)
Duck Secondary Antibodies (3)

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1,426 – 1,440 of 72,126 results
1,426

Donkey anti-Rat IgG (H+L) Secondary Antibody, DyLight 594 (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Rat
Host Species Donkey
Conjugate DyLight 594
Applications Flow Cytometry,Immunomicroscopy
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Flow Cytometry 1:20 - 1:2000, Immunomicroscopy 1:20 - 1:2000
Classification Polyclonal
Immunogen Purified Rat IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on rat IgG and light chains on all rat immunoglobulins. This antibody has been pre-adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit and sheep IgG.
1,427

Donkey anti-Mouse IgG (H+L) Secondary Antibody, DyLight 488 (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Mouse
Host Species Donkey
Conjugate DyLight 488
Applications Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:10000, Immunocytochemistry/Immunofluorescence 1:5000, Fluorophore-linked immunosorbent assay 1:20000
Classification Polyclonal
Immunogen Mouse IgG whole molecule
Reconstitution Reconstitute with 100 ul deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat, and Sheep Serum Proteins. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of mouse IgG and with light chains of most mouse immunoglobulins.
1,429

Donkey anti-Mouse IgG (H+L) Secondary Antibody, DyLight 650, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Mouse
Host Species Donkey
Conjugate DyLight 650
Applications Flow Cytometry,Immunomicroscopy,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Flow Cytometry 1:20 - 1:2000, Immunomicroscopy 1:20 - 1:2000
Classification Polyclonal
Immunogen Purified Mouse IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on mouse IgG and light chains on all mouse immunoglobulins
1,430

Donkey anti-Sheep IgG (H+L) Secondary Antibody [Janelia Fluor 525], Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Sheep
Host Species Donkey
Conjugate Janelia Fluor 525
Applications Western Blot,ELISA,Immunohistochemistry,Immunocytochemistry/Immunofluorescence,Fluorophore-Linked Immunosorbent Assay
Form Purified
Isotype IgG
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) was produced by repeated immunization with Sheep IgG whole molecule in donkey.
Primary or Secondary Secondary
1,431

Donkey anti-Sheep IgG (H+L) Secondary Antibody, Texas Red, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Sheep
Host Species Donkey
Conjugate Texas Red
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Dilution Western Blot 1:1000 - 1:10000, Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Classification Polyclonal
Immunogen Sheep IgG whole molecule
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Sheep IgG and Sheep Serum.
1,432

Invitrogen™ Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight™ 350

Donkey Polyclonal Secondary Antibody

Content And Storage 4°C
Target Species Goat
Host Species Donkey
Conjugate DyLight 350
Applications Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunocytochemistry
Form Liquid
Isotype IgG
Concentration 0.5 mg/mL
Antigen Goat IgG (H+L) Cross-Adsorbed
Regulatory Status RUO
Purification Method Antigen affinity chromatography
Product Type Secondary Antibody
Formulation PBS with 0.09% sodium azide; pH 6.8 to 7.4
Immunogen Goat IgG-heavy and light chain.
Classification Polyclonal
Primary or Secondary Secondary
1,433

Donkey anti-Sheep IgG (H+L) Secondary Antibody, PE, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Sheep
Host Species Donkey
Conjugate PE
Applications Chemiluminescence Immunoassay (CIA),ELISA,Flow Cytometry,Immunoassay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunomicroscopy,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Formulation PBS
Classification Polyclonal
Immunogen This Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against purified sheep IgG (H&L).
Primary or Secondary Secondary
1,434

Donkey anti-Sheep IgG (H+L) Secondary Antibody, HRP, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Sheep
Host Species Donkey
Conjugate HRP
Applications Dot Blot,ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:3000 - 1:15000, ELISA 1:50000 - 1:150000, Immunohistochemistry 1:1000 - 1:5000, Dot Blot
Classification Polyclonal
Immunogen Donkey anti-Sheep IgG (H+L) Secondary Antibody [HRP] was produced by repeated immunization with Sheep IgG whole molecule in donkey.
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Sheep IgG and Sheep Serum.
1,435

Sheep anti-Bovine IgA Secondary Antibody, DyLight 550, Novus Biologicals™
SDP

Sheep Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Conjugate DyLight 550
Target Species Bovine
Host Species Sheep
Applications ELISA,Immunocytochemistry,Immunohistochemistry,Immunohistochemistry (Frozen),Western Blot
Form Purified
Isotype IgG
Antigen IgA
Regulatory Status RUO
Purification Method Affinity purified
Gene Alias Immunoglobulin A
Formulation 50mM Sodium Borate
Gene ID (Entrez) 3493
Classification Polyclonal
Immunogen Purified bovine IgA
Primary or Secondary Secondary
1,436

Sheep anti-Rabbit IgG (H+L) Secondary Antibody, PerCP, Novus Biologicals™
SDP

Sheep Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rabbit
Host Species Sheep
Conjugate PerCP
Applications ELISA,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Formulation PBS
Classification Polyclonal
Immunogen Rabbit IgG whole molecule
Primary or Secondary Secondary
1,437

Sheep anti-Bovine IgA Secondary Antibody, DyLight 755, Novus Biologicals™
SDP

Sheep Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Conjugate DyLight 755
Target Species Bovine
Host Species Sheep
Applications ELISA,Immunocytochemistry,Immunohistochemistry,Immunohistochemistry (Frozen),Western Blot
Form Purified
Isotype IgG
Antigen IgA
Regulatory Status RUO
Purification Method Affinity purified
Gene Alias Immunoglobulin A
Formulation 50mM Sodium Borate
Gene ID (Entrez) 3493
Classification Polyclonal
Immunogen Purified bovine IgA
Primary or Secondary Secondary
1,438

Sheep anti-Bovine IgG1 Heavy Chain Secondary Antibody, HRP, Novus Biologicals™
SDP

Sheep Polyclonal Antibody has been used in 13 publications

Content And Storage Store at 4C. Do not freeze.
Target Species Bovine
Host Species Sheep
Conjugate HRP
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Concentration 1.0 mg/mL
Antigen IgG1 Heavy Chain
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:2000-1:20000, ELISA 1:10000-1:100000, Immunohistochemistry 1:200 - 1:2000, Immunocytochemistry/Immunofluorescence 1:200 - 1:2000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Bovine IgG1
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this reacts specifically with bovine IgG1. This may cross react with IgG1 from other species.
1,439

Invitrogen™ Sheep anti-Rat IgG (H+L) Secondary Antibody, Biotin

Sheep Polyclonal Secondary Antibody

Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target Species Rat
Host Species Sheep
Conjugate Biotin
Applications ELISA,Immunohistochemistry,Western Blot
Form Liquid
Isotype IgG
Concentration 2 mg/mL
Antigen Rat IgG (H+L)
Regulatory Status RUO
Purification Method IgG fraction
Product Type Secondary Antibody
Formulation PBS with 1% BSA and 0.01% sodium azide; pH 7.2
Immunogen Rat IgG whole molecule.
Classification Polyclonal
Primary or Secondary Secondary
1,440

Sheep anti-Canine IgG2 Heavy Chain Secondary Antibody [Janelia Fluor 525], Novus Biologicals™
SDP

Sheep Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Canine
Host Species Sheep
Conjugate Janelia Fluor 525
Applications Western Blot,ELISA,Immunohistochemistry,Immunocytochemistry/Immunofluorescence,Immunohistochemistry (Paraffin)
Form Purified
Isotype IgG
Antigen IgG2 Heavy Chain
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Dog IgG2
Primary or Secondary Secondary