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Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies (44,275)
Mouse Secondary Antibodies (14,507)
Rabbit Secondary Antibodies (6,327)
Donkey Secondary Antibodies (4,321)
Rat Secondary Antibodies (1,240)
Sheep Secondary Antibodies (703)
Chicken Secondary Antibodies (403)
Bovine Secondary Antibodies (257)
Horse Secondary Antibodies (76)
Pig Secondary Antibodies (76)
Alpaca Secondary Antibodies (62)
Hamster Secondary Antibodies (55)
Biosimilar Secondary Antibodies (9)
Human Secondary Antibodies (7)
Duck Secondary Antibodies (3)

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1,441 – 1,455 of 72,126 results
1,441

Donkey anti-Goat IgG (H+L) Secondary Antibody [Janelia Fluor 669], Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Goat
Host Species Donkey
Conjugate Janelia Fluor 669
Applications Western Blot,Flow Cytometry,ELISA,Immunohistochemistry,Immunocytochemistry/Immunofluorescence,Fluorophore-Linked Immunosorbent Assay
Form Purified
Isotype IgG
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Goat IgG whole molecule
Primary or Secondary Secondary
1,442

Donkey anti-Human IgG (H+L) Secondary Antibody, DyLight 680, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Human
Host Species Donkey
Conjugate DyLight 680
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Donkey anti-Human IgG (H+L) Secondary Antibody was developed against human IgG-heavy and light chain.
Primary or Secondary Secondary
1,443

Donkey anti-Rat IgG (H+L) Secondary Antibody [Janelia Fluor 669], Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rat
Host Species Donkey
Conjugate Janelia Fluor 669
Applications Western Blot,ELISA,Immunohistochemistry
Form Purified
Isotype IgG
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Rat IgG whole molecule
Primary or Secondary Secondary
1,444

Donkey anti-Mouse IgG (H+L) Secondary Antibody, Alkaline Phosphatase (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles.
Target Species Mouse
Host Species Donkey
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.50mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Mouse IgG (H&L)
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on mouse IgG and light chains on all mouse immunoglobulins. This antibody has been pre-adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat and sheep IgG.
1,445

Donkey anti-Mouse IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Mouse
Host Species Donkey
Conjugate HRP
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Mouse IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on mouse IgG and light chains on all mouse immunoglobulins. This antibody has been pre-adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat and sheep IgG.
1,446

Donkey anti-Rat IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Rat
Host Species Donkey
Conjugate HRP
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Rat IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on rat IgG and light chains on all rat immunoglobulins. This antibody has been pre-adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit and sheep IgG.
1,448

Donkey anti-Sheep IgG (H+L) Secondary Antibody, DyLight 405, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Sheep
Host Species Donkey
Conjugate DyLight 405
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Donkey anti-Sheep IgG (H+L) Secondary Antibody was developed against sheep IgG-heavy and light chain.
Primary or Secondary Secondary
1,449

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, PerCP, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rabbit
Host Species Donkey
Conjugate PerCP
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation PBS
Classification Polyclonal
Immunogen This Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against rabbit IgG.
Primary or Secondary Secondary
1,450

Donkey anti-Mouse IgG (H+L) Secondary Antibody, Biotin (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Species Mouse
Host Species Donkey
Conjugate Biotin
Applications ELISA,Immunohistochemistry,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0 mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Dilution Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000
Classification Polyclonal
Immunogen Mouse IgG whole molecule
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat, and Sheep Serum Proteins. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
1,451

Donkey anti-Mouse IgG (H+L) Secondary Antibody, Allophycocyanin, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Mouse
Host Species Donkey
Conjugate APC
Applications ELISA,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Formulation PBS
Classification Polyclonal
Immunogen Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) was produced by repeated immunization with Mouse IgG whole molecule in donkey.
Primary or Secondary Secondary
1,452

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Biotin, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Species Rabbit
Host Species Donkey
Conjugate Biotin
Applications ELISA,Immunohistochemistry,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Dilution Western Blot 1:3000 - 1:15000, ELISA 1:300000, Immunohistochemistry 1:500 - 1:3000
Classification Polyclonal
Immunogen Rabbit IgG whole molecule
Primary or Secondary Secondary
Test Specificity Rabbit IgG (H and L)
1,453

Donkey anti-Goat IgG (H+L) Secondary Antibody, FITC (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Goat
Host Species Donkey
Conjugate FITC
Applications Flow Cytometry,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin)
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 0.5 mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:100-1000, Immunohistochemistry-Paraffin 1:100 to 1:1 000, Immunocytochemistry
Classification Polyclonal
Immunogen Goat IgG-heavy and light chain
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive Antibodies. Less than 0.1% cross reactivity to chicken, horse, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
1,454
Promotions Available

Goat IgG (H+L) PE-conjugated Antibody, R&D Systems™
GSA_VA GREENER_CHOICE

Donkey Polyclonal Antibody has been used in 5 publications

Content And Storage Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Target Species Goat
Host Species Donkey
Conjugate R-PE
Applications Flow Cytometry
Isotype IgG
Antigen IgG
Gene Symbols IGHG1
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Flow Cytometry 10 uL/10^6 cells
Gene Alias Immunoglobulin G, ImmunoglobulinG
Gene ID (Entrez) 3500
Immunogen Goat IgG
Classification Polyclonal
Primary or Secondary Secondary
Test Specificity Goat IgG specific IgG was purified by goat IgG (H+L) affinity chromatography.