Synthetic, sequence-verified quantitative standards targeting the mcr-1 gene associated with plasmid-mediated colistin resistance. Supplied at defined concentrations of High – 10,000 copies/µL, Medium – 1,000 copies/µL, and Low – 100 copies/µL, these standards are designed for accurate assay calibration and quantitative performance assessment across a broad dynamic range. They enable robust assay validation, sensitivity and limit-of-detection (LoD) determination, and evaluation of assay linearity in molecular assays. Compatible with real-time PCR and other nucleic acid amplification platforms, the standards ensure consistent inter-run comparability, reliable quantification, and ongoing performance monitoring. Suitable for research, assay development, and routine quality control applications, they support reliable detection and surveillance of clinically significant colistin resistance (RUO)