DNA Cloning and Mapping and Mutagenesis
Thermo Scientific™ Lambda DNA
Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.
Invitrogen™ GeneArt™ Gibson Assembly HiFi Master Mix
GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.
Thermo Scientific™ pUC19 DNA
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
Thermo Scientific™ Rapid DNA Ligation Kit
Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.
Invitrogen™ GeneArt™ Seamless Cloning and Assembly Enzyme Mix
Enables the simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute room temperature reaction
Invitrogen™ GeneArt™ Gibson Assembly EX Master Mix
GeneArt Gibson Assembly EX Master Mix enables DNA assembly via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction.
Thermo Scientific™ pUC18 DNA
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
Invitrogen™ GeneArt™ Type IIs Assembly Kit, Aar I
Provides seamless cloning and assembly of up to 8 DNA fragments by simultaneous cleavage and ligation in a single reaction
Invitrogen™ GeneArt™ pYES1L Vector with Sapphire™ Technology
A ready-to-use, 9.3 Kb linearized BAC/YAC shuttle vector, used for the assembly of DNA fragments with the GeneArt™ High-Order Genetic Assembly System
Thermo Scientific™ Lambda DNA (dam–, dcm–)
Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.
Thermo Scientific™ aLICator LIC Cloning and Expression Kit 3 (C-terminal His-tag)
Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.
Thermo Scientific™ Phusion™ Site-Directed Mutagenesis Kit with DH10B Competent Cells
A versatile and efficient tool for introducing point mutations, insertions, or deletions into any type of plasmid DNA. With this kit the entire plasmid is amplified using phosphorylated primers that introduce the desired changes.
E.coli Genomic DNA
CAS: 9007-49-2
Invitrogen™ GeneArt™ Gibson Assembly HiFi Cloning Kit, chemically competent cells
The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.
Thermo Scientific™ Fast DNA End Repair Kit
Perform blunting and phosphorylation of DNA ends in just 5 minutes for subsequent use in blunt-end ligation with this kit.