The intracellular levels of NADP+ and NADPH are recognized as important metabolic markers in understanding how cancer cells & mitochondrial function are influenced by drug administration, genetic engineering, etc. Cell lysate from a cell culture can be easily prepared via Deproteinzation using the extraction buffer and filtration tubes found within this kit. Intracellular NADPH levels can be quantified by heat treatment of cell lysate. NADP/NADPH Assay Kit-WST enables quantitation of the amount of total NADP+/NADPH, NADPH and NADP+ in cells and measurement of their ratio. Additionally, intracellular NADP+ levels can be determined by subtracting NADPH levels from independently measured total NADP+/NADPH levels. Dojindo has various quantification kits of Intracellular Metabolites. (https://www.dojindo.com/quantification-of-intracellular-metabolites/)